Platelet-Rich Blood Derivatives for Stem Cell-Based Tissue Engineering and Regeneration

Masoudi, E.A.; Ribas, João Francisco Magno; Kaushik, Gaurav; Leijten, Jeroen; Khademhosseini, Ali

doi:10.1007/s40778-016-0034-8

Cited by 82 publications

(57 citation statements)

References 77 publications

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“…PRP had consistently shown to potentiate stem cell proliferation, migration, and differentiation (Masoudi et al [33]). The PRP peptide GFs such as EGF were the principal external indicators that activate the mitogen-activated protein kinase and had a role in trans-differentiation of pancreatic acinar and ductal cells into endocrine islet cells (AbbanMete et al [34]).…”

Section: Discussionmentioning

confidence: 99%

Effect of Platelet Rich Plasma (PRP) Injection on the Endocrine Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study

El‐Tahawy¹,

Rifaai²,

Saber³

et al. 2017

J Diabetes Metab

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Introduction: Diabetes mellitus is a global problem and several restoration approaches have been developed to induce beta (β) cells regeneration. Platelet rich plasma (PRP) is an autogenous and economical source of growth factors which nowadays used in the tissue repair. Aim:To test the hypothesis that PRP could play a role in the improvement of the structural changes occurred in the endocrine pancreas of experimentally-induced diabetic rats and the possible mechanisms through which PRP induced its effects to shed a light on the possible use of such application in the clinical field. Material and methods:Sixty male albino rats were used; 20 for obtaining the PRP and 40 were divided into 4 equal groups (10 rats each): control, PRP-group, diabetic group, PRP/diabetic group. Diabetes was induced by single intra-peritoneal injection of streptozotocin (50-60 mg/kg). The PRP was administered by SC injections in a dose of 0.5 mg/kg twice weekly for 3 weeks. Results:The diabetic group showed a significant increase in blood glucose levels compared to the control. Treatment with PRP significantly reduced the blood glucose levels compared to the diabetic group. The diabetic group showed variable marked morphological changes which diminished by the PRP administration. PRP/diabetic group had a significant increase in the mean number of pancreatic islets and β-cells/islet compared to the diabetic group. The islet cells appeared normal with scarcely seen vacuolations. The duct system showed several changes; stratifications, invagination of the surface epithelium to the underlying connective tissue, and sprouting of the ductal epithelial cells in between the lobules. Numerous small islets were noticed in a close association with the intralobular ducts. Small newly lobules with abundant connective tissue were organized. There were significant increases in the insulin immunopositive β-cells and PCNA positive cells in PRP/diabetic group compared to diabetic group. Conclusion:This study provides an evidence of the diabetic pancreatic islet regeneration in response to PRP treatment. The PRP stimulated islet cell regeneration and stimulated the induction of other sources of β-cells generation as the exocrine portion of the pancreas; ductal and acinar cells. In addition, PRP might put the pancreas into an environment similar to the postnatal developmental one where new lobules were formed. These will pave the future for a novel treatment for diabetes.

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Section: Discussionmentioning

confidence: 99%

Effect of Platelet Rich Plasma (PRP) Injection on the Endocrine Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study

El‐Tahawy¹,

Rifaai²,

Saber³

et al. 2017

J Diabetes Metab

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“…Platelet-rich plasma was prepared using two centrifugation steps (Lahmy et al, 2014;Tang et al, 2015;Burnouf et al, 2016;Masoudi et al, 2016). Briefly, 10 ml human fresh blood was collected into tubes containing 1 ml acid-citrate-dextrose solution as an anticoagulant.…”

Section: Preparation Of Platelet-rich Plasmamentioning

confidence: 99%

“…However, the shortage of pancreatic islets from cadavers and immune rejection and ethical issues are considered major problems to the transplantation of islets. The concept that PRP could promote cell differentiation and tissue regeneration is based on the fact that platelets release role of platelet-released a variety of growth factors and adhesion molecules that include molecules, including platelet-derived growth factor (PDGF), insulin-like growth factor-1 (IGF-1), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and epidermal growth factor (EGF), hepatocyte growth factor (HGF), vitronectin, fibronectin, and other factors that are secreted by the platelet granules (S anchez-Gonz alez et al, 2012;Masoudi et al, 2016). Accordingly, several reports in stem cell biology have raised extremely promising results for the generation of IPCs by differentiation of various sources types of stem cells (Vaca et al, 2006;Sawangmake et al, 2014;Xu et al, 2015b).…”

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confidence: 99%

“…PRP is a portion of the whole blood that can be extracted by the centrifugation and contains a high concentration of platelets and fibrin, that has now been shown to have a potent effect in stimulating the differentiation of stem cells into blood vessels, and tendon, bone, and cartilage cells (Zhang and Wang, 2010;Teixeira et al, 2012;Xu et al, 2015a). The concept that PRP could promote cell differentiation and tissue regeneration is based on the fact that platelets release role of platelet-released a variety of growth factors and adhesion molecules that include molecules, including platelet-derived growth factor (PDGF), insulin-like growth factor-1 (IGF-1), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and epidermal growth factor (EGF), hepatocyte growth factor (HGF), vitronectin, fibronectin, and other factors that are secreted by the platelet granules (S anchez-Gonz alez et al, 2012;Masoudi et al, 2016). The growth factors and adhesion molecules present in PRP may play an effective role in the differentiation of stem cells into IPCs.…”

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confidence: 99%

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Generation of Insulin‐Producing Cells From Human‐Induced Pluripotent Stem Cells Using a Stepwise Differentiation Protocol Optimized With Platelet‐Rich Plasma

Enderami

Mortazavi

Soleimani

et al. 2017

Journal Cellular Physiology

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Studies on patient-specific human-induced pluripotent stem cells (hiPSCs) as well as a series of autologous growth factors presumably will reveal their many benefits for cell base replacement therapy in type 1 diabetes mellitus (TIDM) patients in the future. For this purpose, we established a multistep protocol by adding platelet-rich plasma (PRP) that induce the hiPSCs into insulin-producing cells (IPCs). We present here a differentiation protocol consisting of five stages in two groups including protocol with PRP and without PRP. Charac-teristics of derived IPCs in both groups were evaluated at the mRNA and protein levels, cell cycle and viability in the end stage of cell differentiation. The in vitro studies indicated the treatment of hiPSCs in the protocol with PRP resulting in differentiated cells with strong characteristics of IPCs including islet-like cells, the expression of mature and functional pancreatic beta cell specific marker genes. In addition to these pancreatic specific markers were detected by immunochemistry and Western blot. Our differentiated cells in two groups secreted insulin and C-peptide in a glucose stimulation test by ELISA showing in vitro functional. The results of the cell cycle assay confirmed that differentiation has been done. We reported for the first time that PRP might be ideal additive in the culture medium to induce pancreatic differentiation in the hiPSCs. This study provides a new approach to investigate the role of PRP in pancreatic differentiation protocols and enhance the feasibility of using patient-specific iPSCs and autologous PRP for future beta cells replacement therapies for T1DM. J. Cell. Physiol. 232: 2878-2886, 2017. © 2016 Wiley Periodicals, Inc.

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“…In fact, PLTrich blood derivatives, such as PLT-rich plasma, PLT-rich fibrin or PLT releasate, are applied for stem cell-based tissue engineering and regeneration [30]. Moreover, the clinical use of autologous PLT products is proven in the application of PLT lysate drops to prevent graft-versus-host disease (GvHD) [31] or support wound healing in ophthalmology [32].…”

Section: Discussionmentioning

confidence: 99%

Towards the Manufacture of Megakaryocytes and Platelets for Clinical Application

Baigger¹,

Blasczyk²,

Figueiredo³

2017

Transfus Med Hemother

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Platelet transfusions are used in standard clinical practice to prevent hemorrhage in patients suffering from thrombocytopenia or platelet dysfunctions. Recently, a constant rise on the demand of platelets for transfusion has been registered. This may be associated with several factors including demographic changes, population aging as well as incidence and prevalence of hematological diseases. In addition, platelet-regenerative properties have been started to be exploited in different areas such as tissue remodeling and anti-cancer therapies. These new applications are also expected to increase the future demand on platelets. Thus, in vitro generated platelets may constitute a highly desirable alternative to meet the rising demand on platelets. Several factors have been considered in the road trip of producing in vitro megakaryocytes and platelets for clinical application. From selection of the cell source, differentiation protocols and culture conditions to the design of optimal bioreactors, several strategies have been proposed to maximize production yields while preserving functionality. This review summarizes new advances in megakaryocyte and platelet differentiation and their production upscaling.

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Platelet-Rich Blood Derivatives for Stem Cell-Based Tissue Engineering and Regeneration

Cited by 82 publications

References 77 publications

Effect of Platelet Rich Plasma (PRP) Injection on the Endocrine Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study

Effect of Platelet Rich Plasma (PRP) Injection on the Endocrine Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study

Generation of Insulin‐Producing Cells From Human‐Induced Pluripotent Stem Cells Using a Stepwise Differentiation Protocol Optimized With Platelet‐Rich Plasma

Towards the Manufacture of Megakaryocytes and Platelets for Clinical Application

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