Platelet polyphosphate level is elevated in patients with chronic primary thrombocytopenia: A preliminary study

Żurawska-Płaksej, Ewa; Kuliczkowski, Wiktor; Karolko, Bożena; Cielecka-Prynda, Magdalena; Dębski, Jakub; Kaaz, Konrad; Mysiak, Andrzej; Wróbel, Tomasz; Podolak-Dawidziak, Maria; Usnarska−Zubkiewicz, Lidia

doi:10.17219/acem/125430

Cited by 4 publications

(2 citation statements)

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“…In fact, not only does vascular smooth muscle contraction reduce the diameter of injured vessels as a mechanism of reflex, but also activation of the coagulation cascade allows platelets to form a clot with fibronectin, fibrin, vitronectin and thrombospondin [ 59 ]. Platelets also release several growth factors and cytokines when degranulating [ 60 ]. Growth factors and cytokines such as PDGF (platelet-derived growth factor), TGFβ (transforming growth factor β) and EGF (epidermal growth factor) activate neutrophils, macrophages, endothelial cells, fibroblasts and keratinocytes [ 61 ].…”

Section: Wound Healingmentioning

confidence: 99%

Obesity and Wound Healing: Focus on Mesenchymal Stem Cells

Alma

Marconi

Rossi

et al. 2023

Life

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Chronic wounds represent nowadays a major challenge for both clinicians and researchers in the regenerative setting. Obesity represents one of the major comorbidities in patients affected by chronic ulcers and therefore diverse studies aimed at assessing possible links between these two morbid conditions are currently ongoing. In particular, adipose tissue has recently been described as having metabolic and endocrine functions rather than serving as a mere fat storage deposit. In this setting, adipose-derived stem cells, a peculiar subset of mesenchymal stromal/stem cells (MSCs) located in adipose tissue, have been demonstrated to possess regenerative and immunological functions with a key role in regulating both adipocyte function and skin regeneration. The aim of the present review is to give an overview of the most recent findings on wound healing, with a special focus on adipose tissue biology and obesity.

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Section: Wound Healingmentioning

confidence: 99%

Obesity and Wound Healing: Focus on Mesenchymal Stem Cells

Alma

Marconi

Rossi

et al. 2023

Life

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“…2021, 22, 7257 2 of 13 polyP is thought to be involved in several major physiological functions. However, due to the lack of standardized quantification methods, both platelet polyP levels and dynamic mobilization have rarely been reported and cannot be compared using data obtained by different quantification methods [11].…”

Section: Introductionmentioning

confidence: 99%

Fluorometric Quantification of Human Platelet Polyphosphate Using 4′,6-Diamidine-2-phenylindole Dihydrochloride: Applications in the Japanese Population

Watanabe

Kitamura

Aizawa

et al. 2021

IJMS

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Polyphosphate (polyP), a biopolymer of inorganic phosphate, is widely distributed in living organisms. In platelets, polyP is released upon activation and plays important roles in coagulation and tissue regeneration. However, the lack of a specific quantification method has delayed the in-depth study of polyP. The fluorescent dye 4′,6-diamidine-2-phenylindole dihydrochloride (DAPI) has recently received attention as a promising probe for the visualization and quantification of cellular polyP levels. In this study, we further optimized quantification conditions and applied this protocol in quantification of platelet polyP levels in a Japanese population. Blood samples were collected from non-smoking, healthy Japanese subjects (23 males, 23 females). Washed platelets were fixed and probed with DAPI for fluorometric determination. PolyP levels per platelet count were significantly higher in women than that in men. A moderate negative correlation between age and polyP levels was found in women. Responsiveness to CaCl2 stimulation was also significantly higher in women than that in men. Overall, our optimized protocol requires neither purification nor degradation steps, reducing both the time and bias for reproducible quantification. Thus, we suggest that despite its low specificity, this DAPI-based protocol would be useful in routine laboratory testing to quantify platelet polyP levels efficiently and economically.

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Inorganic Polyphosphate, Mitochondria, and Neurodegeneration

Urquiza

Solesio

2022

Inorganic Polyphosphates

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Platelet polyphosphate level is elevated in patients with chronic primary thrombocytopenia: A preliminary study

Cited by 4 publications

References 0 publications

Obesity and Wound Healing: Focus on Mesenchymal Stem Cells

Obesity and Wound Healing: Focus on Mesenchymal Stem Cells

Fluorometric Quantification of Human Platelet Polyphosphate Using 4′,6-Diamidine-2-phenylindole Dihydrochloride: Applications in the Japanese Population

Inorganic Polyphosphate, Mitochondria, and Neurodegeneration

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