Platelet parameters and expression of platelet membrane glycoprotein in childhood acute lymphoblastic leukemia

Huang, Zhanwen; Liu, W J; Guo, Qulian; Liu, C Y

doi:10.4238/2015.december.7.20

Cited by 12 publications

(11 citation statements)

References 25 publications

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“…Subsequently, 1% paraformaldehyde was added (2–8°C, 1 ml) into each tube, mixed completely, and placed at 2–8°C in the dark for 30 min. Using FCM, signal for 10,000 platelets was acquired, and the positive expression rate of CD62p, PAC-1 and CD42b was calculated ( 19 ).…”

Section: Methodsmentioning

confidence: 99%

Role of platelet function and platelet membrane glycoproteins in children with primary immune thrombocytopenia

Chen

Jing

Guo

et al. 2016

Molecular Medicine Reports

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The aim of the present study was to examine and understand changes in platelet functions prior to and after the treatment of primary immune thrombocytopenia (ITP) in children. An automatic hematology analyzer and whole blood flow cytometry were used to detect immature platelet fraction (IPF), IPC and membrane glycoproteins (CD62p, PAC-1 and CD42b) in ITP children (ITP group), children with complete response after ITP treatment (ITP-CR group) and children with elective surgery (normal control group). The results showed that, levels of platelet count (PLT) and plateletcrit in the ITP group were lower alhtough the levels of mean platelet volume, platelet distribution width and platelet-large cell ratio (P-LCR) were higher than those in the normal control and ITP-CR groups. PLT in the ITP-CR group was lower than that in the normal controls. Additionally, IPF% was higher in the normal control and ITP-CR groups, IPC was lower in the ITP group compared to the normal control and ITP-CR groups. Furthermore, prior to ADP activation, the expression levels of CD62p, PAC-1 and CD42b in the ITP group were lower in ITP group than those in the normal control and ITP-CR groups. The expression level of PAC-1 was lower in the ITP-CR and normal control groups. No differences were identified in CD62p and CD42b expression levels. Following ATP activation, CD62p, PAC-1 and CD42b expression in the ITP group was lower than that in the normal control and ITP-CR groups. PAC-1 expression was lower while CD62p expression was higher in the ITP-CR group compared to the normal control group. In conclusion, the activation of platelets in ITP children was low. Decreased platelet function, platelet parameters and platelet glycoproteins may be used as markers for monitoring the treatment efficacy in ITP children.

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Section: Methodsmentioning

confidence: 99%

Role of platelet function and platelet membrane glycoproteins in children with primary immune thrombocytopenia

Chen

Jing

Guo

et al. 2016

Molecular Medicine Reports

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“…Acute lymphoblastic leukemia (ALL) is a neoplastic disease originating from B- or T-lymphocytes ( 1 – 2 ), and accounts for 80% of childhood acute leukemia (AL) and is the most common type of cancer affecting children ( 3 , 4 ). The incidence rate of ALL is 5-fold higher than that of acute myeloid leukemia (AML) ( 3 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of ATRA on the expression of HOXA5 gene in K562 cells and its relationship with cell cycle and apoptosis

Chen

Zhang

Guo

et al. 2016

Molecular Medicine Reports

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Leukemia is the most common malignant disease in children with high incidence and mortality rates, and a poor treatment effect. The aim of the present study was to examine the changes in the expression of homeobox (Hox) A5 gene and its relationship with cell cycle and apoptosis through the intervention of human K562 myeloid leukemia cell line by all-trans retinoic acid (ATRA), to analyze the role of HOXA5 in the pathogenesis and development process of myeloid leukemia. The optimal concentration of ATRA to be used with K562 cells was determined using a cell counting kit-8 (CCK-8). After 24, 72 and 48 h following treatment of K562 cells with 10 µmol/l ATRA, cell cycle events and apoptosis were measured using flow cytometry. HOXA5 mRNA and protein expression in K562 cells was assessed by RT-PCR and western blot analysis, and the relationship between HOXA5 expression and cell cycle and apoptosis was analyzed. The HOXA5 mRNA and protein expression levels were increased following treatment with ATRA in K562 cells. Apoptosis was increased significantly. The cell cycle was inhibited in G0/G1 phase. Cell proliferation was also inhibited. HOXA5 mRNA and protein expression rates positively correlated with cell apoptosis and the increased percentage and cell cycle of the G0/G1 phase. However, HOXA5 negatively correlated with the reduced percentage of S stage. In conclusion, the expression of HOXA5 in cells was increased following treatment with ATRA in K562 cells, in a time-dependent manner. Additionally, ATRA may inhibit the proliferation of K562 cells and promote apoptosis by upregulating the HOXA5 mRNA and protein expression.

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“…PDW above 18% was described as increasing the risk of mortality [Guclu et al, 2013]. There are also studies reporting low PDW values [Liang et al, 2014;Huang et al, 2015]. PDW was significantly lower in the patient group compared to the control group on Alzheimer's disease and vascular dementia cases [Liang et al, 2014].…”

Section: Discussionmentioning

confidence: 99%

Prognostic impact of platelet distribution width in patients with Crimean-Congo hemorrhagic fever

Yılmaz

Menteşe

et al. 2016

J. Med. Virol.

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Platelet distribution width (PDW) is a readily available blood test involving calculations performed by automated blood analyzers. Crimean-Congo hemorrhagic fever (CCHF) may exhibit a severe profile with fatal hemorrhaging or else present with a mild clinical process. The purpose of our study was to investigate the importance of PDW in CCHF patients and its clinical prognostic value. This study was conducted with patients with CCHF. Patients were divided into two groups on the basis of presence or absence of bleeding. Demographic characteristics, clinical findings, PDW, and other laboratory tests were recorded onto forms. A total of 423 patients were included. Hemorrhaging was observed in 27.9% during hospitalization. PDW on the first day of hospitalization was 17.2 ± 0.9% in the hemorrhagic patients and 17.1 ± 0.6% in the cases without hemorrhage (P = 0.290). On the third day of hospitalization, PDW was 17.6 ± 0.8% in the hemorrhagic patients and 17.0 ± 0.7% in the cases without hemorrhage (P < 0.001). At a third-day PDW level cut-off point of 17.1%, AUROC was 0.677, sensitivity 65.5%, specificity 54.6%, PPV 35.5%, and NPV 80.6%. A one-unit raise in third day PDW stepped up the probability of bleeding in patients with CCHF 3.45-fold at logistic regression analysis. This study shows that PDW is a parameter that may be used to determine disease severity. This parameter may be at least as useful as platelet count in helping clinicians identify severe cases. Early identification of cases with a severe course will make it possible to provide early planning of modalities such as intensive care support. J. Med. Virol. 88:1862-1866, 2016. © 2016 Wiley Periodicals, Inc.

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Platelet parameters and expression of platelet membrane glycoprotein in childhood acute lymphoblastic leukemia

Cited by 12 publications

References 25 publications

Role of platelet function and platelet membrane glycoproteins in children with primary immune thrombocytopenia

Role of platelet function and platelet membrane glycoproteins in children with primary immune thrombocytopenia

Effect of ATRA on the expression of HOXA5 gene in K562 cells and its relationship with cell cycle and apoptosis

Prognostic impact of platelet distribution width in patients with Crimean-Congo hemorrhagic fever

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