Platelet microvesicles adhere to subendothelium and promote adhesion of platelets

Owens, Michael R.; Holme, Stein; Cardinali, Sharon

doi:10.1016/0049-3848(92)90195-g

Cited by 50 publications

(24 citation statements)

References 18 publications

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“…Infusible platelet membranes (IPM) prepared from new or outdated human platelets have been developed as an alternative to standard PCs. These microparticles have additional advantages like long shelf life, increased viral safety and decreased adverse reactions due to the removal of plasma [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

HLA Antigens Shed from the Surface of Synthetic or Naturally Occurred Platelet-Derived Microparticles During Storage of Platelet Concentrate

Yari

Ahmadzadeh

Azadpour

et al. 2011

Indian J Hematol Blood Transfus

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The demand for standard platelet concentrates (PCs) has continued to increase in the recent years. Infusible platelet membranes (IPM) prepared from new or outdated human platelets have been developed as an alternative to standard PCs, with the additional advantage of long shelf life and increased viral safety. Reduction of HLA antigens on the IPM has been assigned as one of the probable advantages of this product. In re-examining this issue, we studied the existence of HLA class I on the surface of IPM microparticles. In comparison we also surveyed HLA expression on the surface of the naturally occurred platelet-derived microparticles (nPMPs) during 7 days storage. Intended for producing IPM, PCs obtained from Iranian blood transfusion organization were lysed; virally inactivated with wet heat in the presence of a heat stabilizer and then sonicated. IPMs were separated using centrifugation and liquid-stored in 4°C. The expression of HLA class I antigens was surveyed using flow cytometry technique. HLA molecules were present on the microparticles. Shedding of HLA antigens was demonstrated from the surface of the both liquid-stored IPM and nPMPs during storage. Storage of IPM in 4°C was accompanied with significant reduction of HLA molecules. It seemed that achievement of HLA-free IPM could be impossible unless chloroquine treated platelets were used to prepare these microvesicles.

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Section: Introductionmentioning

confidence: 99%

HLA Antigens Shed from the Surface of Synthetic or Naturally Occurred Platelet-Derived Microparticles During Storage of Platelet Concentrate

Yari

Ahmadzadeh

Azadpour

et al. 2011

Indian J Hematol Blood Transfus

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“…Garnitur receptorowy MV łączy się z ich pochodzeniem, jako że na powierzchni MV pochodzących z płytek krwi występują receptory dla integryny (β 3 , α IIβ, α IIbβ 3 ), P-selektyny oraz cząstek różnicowania CD42b, 41, 41a, 42a, 61, 62P, zaś na powierzchni MV pochodzących z erytrocytów -glikoforyna A i CD235a, a w przypadku MV pochodzących z monocytów -receptor dla LPS i CD14, zaś na powierzchni MV pochodzących z komórek środbłonka specyficznymi receptorami są PECAM-1 (platelet endothelial cel adhesion molecule), GP 105-110, E-selektyna, αv integryna, VE-kaderyna, endoglina i aneksyna V oraz receptory różnicowania CD31, 34, 62E, 51, 146, 144, 105 (13,28). Rola MV wiąże się nie tylko z krzepnięciem krwi, adhezją, fibrynolizą i angiogenezą, ale także z ich udziałem w procesach zapalnych (3,4,8,13,15,21,28,30) oraz w regulacji apoptozy (2,30). W przypadku MV pochodzących z płytek krwi wykazano, że zachowują one zdolność prokoagulacyjną, podobnie jak tromboplastyna (13,30).…”

Section: Charakterystyka Mikropęcherzykówunclassified

“…Inną ważną rolą MV jest ich udział w adhezji do innych komórek, np. komórek śródbłonka, który to proces modulowany jest dzięki obecności na powierzchni MV takich czynników, jak: PECAM-1, ICAM-1, E-selektyna, VE-kadheryna, S-endo i endoglina (21,28). Wykazano (21), że MV wyposażone we wspomniane receptory niezbędne w procesie adhezji, zachowują się analogicznie jak płytki krwi i dość łatwo przylegają do śródbłonka.…”

Section: Charakterystyka Mikropęcherzykówunclassified

“…komórek śródbłonka, który to proces modulowany jest dzięki obecności na powierzchni MV takich czynników, jak: PECAM-1, ICAM-1, E-selektyna, VE-kadheryna, S-endo i endoglina (21,28). Wykazano (21), że MV wyposażone we wspomniane receptory niezbędne w procesie adhezji, zachowują się analogicznie jak płytki krwi i dość łatwo przylegają do śródbłonka. Na podobnej zasadzie struktury MV biorą udział w fibrynolizie, dzięki obecności na swojej powierzchni uPAR (urokinase plasminogen activator receptor) i uPA (urokinase-type plasminogen activator) (9,28).…”

Section: Charakterystyka Mikropęcherzykówunclassified

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Microvesicles and exosome granules and their role in macroorganism.

Niedźwiedzka-Rystwej¹,

Tokarz–Deptuła²,

Deptuła³

2017

Medycyna Weterynaryjna

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Niedźwiedzka-Rystwej P., Tokarz-Deptuła B., Deptuła W. Microvesicles and exosome granules and their role in macroorganism SummaryIn recent years, it has been discovered that many membrane elements in cells are crucial for homeostasis and constitute a pivotal, previously ignored, element of immunity. Such elements are microvesicles (MV) and exosome granules (EG), which for years have been confused with each other because of their similarity and imprecise nomenclature. Today, however, it is known, that these structures differ in their phenotypes and functions. MV are structures released by the morphotic elements of blood, thrombocytes, erythrocytes, monocytes, granulocytes, lymphocytes and vascular endothelium cells, and they play a role in blood clotting, adhesion, fibrinolysis and angiogenesis, as well as in inflammation and apoptosis. EG, on the other hand, belong to RNA granules, which protect genetic material in cells and are also involved in inflammation, immunity, autoimmune disorders, and cancer.

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“…On the other hand, the incubation of plasma (or serum) with Plt prior to the solubilization step may result in a concentration of plasma Ab in the capture assays. Moreover, the presence of platelet microparticles (PMP) (Wenche et al, 1992;Owens & Dunn, 1990a), which are known to be increased in the plasma of patients with ITP (Wenche et al, 1992;Zucker-Franklin & Karpatkin, 1977), have to be taken into consideration. Ab associated with Plt are very likely to be bound to PMP as well.…”

Section: Discussionmentioning

confidence: 99%

Quantitation of platelet‐specific autoantibodies in platelet eluates of ITP patients measured by a novel ELISA using the purified glycoprotein complexes GPIIb/IIIa and GPIb/IX as antigens

Hurlimann-Forster¹,

Steiner

Felten³

1997

Br J Haematol

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Summary.Immune thrombocytopenic purpura (ITP) is a disorder caused by anti-platelet autoantibodies (Ab), most of which are directed against epitopes on platelet membrane glycoprotein complexes GPIIb/IIIa and GPIb/IX. To detect platelet Ab, reliable techniques, such as MAIPA or immunobead assay, have been developed. They all achieve their selective specificity by the use of monoclonal antibodies (MoAb) against defined glycoproteins of the platelet membrane. In order to determine the most frequent Abspecificities, a novel enzyme-linked immunosorbent assay, named platelet-glycoprotein-ELISA (P-GP-ELISA), has been developed. It uses purified GPIIb/IIIa and GPIb/IX complexes, respectively, as antigens and enables determination of platelet-associated as well as circulating Ab (IgG, IgM).MoAbs are not required and therefore there is no risk of competition between MoAb and Ab. Levels of Ab in patients with the clinical diagnosis of an idiopathic thrombocytopenic purpura were analysed. 92 . 7% (76/82) platelet eluates with significantly increased levels of Ab against at least one of the glycoproteins were found, whereas no sample from healthy volunteers (0/37) gave a positive result, pointing to a high sensitivity and specificity of the test system. Since its application is also easy and quick, P-GP-ELISA should facilitate detection of Ab against platelet membrane proteins in routine determinations.

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Platelet microvesicles adhere to subendothelium and promote adhesion of platelets

Cited by 50 publications

References 18 publications

HLA Antigens Shed from the Surface of Synthetic or Naturally Occurred Platelet-Derived Microparticles During Storage of Platelet Concentrate

HLA Antigens Shed from the Surface of Synthetic or Naturally Occurred Platelet-Derived Microparticles During Storage of Platelet Concentrate

Microvesicles and exosome granules and their role in macroorganism.

Quantitation of platelet‐specific autoantibodies in platelet eluates of ITP patients measured by a novel ELISA using the purified glycoprotein complexes GPIIb/IIIa and GPIb/IX as antigens

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