Platelet Microparticle Controversial Role in Cancer

Nazari, Mahnaz; Javandoost, Ehsan; Talebi, Mehdi; Movassaghpour, Aliakbar; Soleimani, Masoud

doi:10.34172/apb.2021.005

Cited by 9 publications

(5 citation statements)

References 180 publications

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“…Importantly, the authors found an elevated concentration of these particles in circulation of gastric cancer patients, with remarkably increased numbers at advanced disease stages [ 145 ]. Since then, numerous works have investigated platelet-derived EVs and their alteration has been linked to several cancers [ 146 ]. Increased levels of total EVs or of a specific subtype of EVs in blood of patients with different types of tumours—such as melanoma, prostate cancer, and various haematological tumours—have been reported by many works, and the potential use of these measures as tumour markers is being discussed [ 147 ].…”

Section: Implication Of Circulating Extracellular Vesicles In Pathological Processesmentioning

confidence: 99%

Extracellular Vesicles in Blood: Sources, Effects, and Applications

Alberro

Iparraguirre

Fernandes

et al. 2021

IJMS

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Extracellular vesicles (EVs) are important players for intercellular communication. EVs are secreted by almost all cell types; they can transfer information between nearby or distant cells, and they are highly abundant in body fluids. In this review, we describe the general characteristics of EVs, as well as isolation and characterization approaches. Then, we focus on one of the most relevant sources of EVs: the blood. Indeed, apart from EVs secreted by blood cells, EVs of diverse origins travel in the bloodstream. We present the numerous types of EVs that have been found in circulation. Besides, the implications of blood-derived EVs in both physiological and pathological processes are summarized, highlighting their potential as biomarkers for the diagnosis, treatment monitoring, and prognosis of several diseases, and also as indicators of physiological modifications. Finally, the applications of EVs introduced in the circulatory system are discussed. We describe the use of EVs from distinct origins, naturally produced or engineered, autologous, allogeneic, or even from different species and the effects they have when introduced in circulation. Therefore, the present work provides a comprehensive overview of the components, effects, and applications of EVs in blood.

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Section: Implication Of Circulating Extracellular Vesicles In Pathological Processesmentioning

confidence: 99%

Extracellular Vesicles in Blood: Sources, Effects, and Applications

Alberro

Iparraguirre

Fernandes

et al. 2021

IJMS

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show abstract

“…studies have shown that PMPs can result in either cancer progression or suppression of tumor growth. [27] Our ndings support the inhibitory role of PMPs on cancer cells in vitro However, the exact mechanism underlying these effects remains unclear, and further studies are needed to elucidate it. Moreover, our study highlights the usefulness of using platelet microparticles, which are abundant and easily separable, for studying cancer biology and developing new treatment protocols.…”

Section: Discussionmentioning

confidence: 55%

Platelet microparticles influence gene expression and modulate biological activities of chronic myeloid leukemia cells (K562)

Nikravesh,

Mirzaee Khalilabadi,

Farsinejad

et al. 2024

Mol Biol Rep

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BackgroundThe current understanding emphasizes the intricate interplay between leukemia and its environment. Platelet microparticles play a crucial role in facilitating intercellular communication and contribute to the complex landscape of cancer pathology. This study aimed to investigate the in uence of Platelet microparticles on cell proliferation, apoptosis, and the expression of key genes, including P53, P21, Cyclin D1, Bax, and Bcl-2, within the context of a Chronic Myeloid Leukemia cell line (K562). Methods and ResultsPlatelet microparticles were obtained through centrifugation at various speeds, and their concentration was quanti ed using the BCA assay. To determine the size and immunophenotypic characteristics of the PMPs, both the DLS technique and ow cytometry were employed. Cell proliferation was assessed using the MTT assay, and cell cycle analysis was conducted through DNA content evaluation. Real-time PCR was utilized for gene expression analysis of Bax, Bcl-2, Cyclin D1, P53, and P21. Flow cytometry was employed to examine cell apoptosis. The ndings revealed that platelet microparticles have the ability to decrease proliferation of the K562 cell line, while not exerting an impact on apoptosis and cell cycle progression. Analysis through real-time PCR indicated an upregulation in the gene expression of P53, P21, and Bcl-2, accompanied by a downregulation in Bax and Cyclin D1. ConclusionThis investigation sheds light on the intricate relationship between CML and its microenvironment, particularly the involvement of platelet-derived microparticles. The study underscores the potential of PMPs to in uence cell behavior and gene expression, providing a deeper understanding of their role in CML and its therapeutic implications.

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“…Platelet and TEP-derived EVs generate a positive feedback loop that ampli es platelet education and contributes to tumour growth. These EVs may also participate in metastatic processes as platelets do, however irrefutable evidence is still lacking (72)(73)(74). Such ideas support the signi cance of platelet-derived EVs as valuable biomarker sources, which may actually be more enriched in disease-speci c analytes than the actual platelets generating them.…”

Section: Discussionmentioning

confidence: 97%

Selective isolation of extracellular vesicles from minimally processed human plasma as a translational strategy for liquid biopsies

Fortunato¹,

Giannoukakos

Giménez‐Capitán³

et al. 2022

Preprint

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Background: Intercellular communication is mediated by extracellular vesicles (EVs), as they enclose selectively packaged biomolecules that can be horizontally transferred from donor to recipient cells. Because all cells constantly generate and recycle EVs, they provide accurate timed snapshots of individual pathophysiological status. Although EVs can be isolated from any biofluid, blood plasma circulates through the whole body and it potentially transports EVs derived from most organs, making it the biofluid of choice in most studies. Blood collection is easy and minimally invasive, yet reproducible procedures to obtain pure bulk EV samples and specific EV subtypes from blood are still lacking. Here, we addressed central aspects of EV immunoaffinity isolation from simple and complex matrices, such as plasma. Methods/Results: Fluorescent EV spiking allowed us to demonstrate that target EV subpopulations can be efficiently retrieved from plasma, and that their enrichment is dependent not only on complex matrix composition, but also on the EV surface phenotype. Additionally, we found that plasma-derived EVs can be captured and detected using a simple protocol, which sequentially combines isolation and staining of specific surface markers. Finally, we conducted mRNA profiling experiments to prove that distinct EV subpopulations can be captured by directly targeting different surface markers. Furthermore, platelet-derived EVs encapsulated mRNA expression patterns that might be associated to early-stage lung cancer, which demonstrated that each EV subset conferred a differential clinical value, highlighting the advantages of selective isolation.Conclusions: In summary, our EV isolation protocol facilitated the extraction of clinically useful information from plasma. Compatible with common downstream analytics, it is a readily implementable tool that was tailored to provide a truly translational solution in routine clinical workflows, fostering the inclusion of EVs in novel liquid biopsy settings.

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Platelet Microparticle Controversial Role in Cancer

Cited by 9 publications

References 180 publications

Extracellular Vesicles in Blood: Sources, Effects, and Applications

Extracellular Vesicles in Blood: Sources, Effects, and Applications

Platelet microparticles influence gene expression and modulate biological activities of chronic myeloid leukemia cells (K562)

Selective isolation of extracellular vesicles from minimally processed human plasma as a translational strategy for liquid biopsies

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