Platelet lysate stimulates wound repair of HaCaT keratinocytes

Ranzato, Elia; Patrone, Mauro; Mazzucco, Laura; Burlando, Bruno

doi:10.1111/j.1365-2133.2008.08699.x

Cited by 44 publications

(72 citation statements)

References 42 publications

(45 reference statements)

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“…Such a view is supported by data from the cell migration assay, which showed a chemoattractant activity of PL on HuVEC. These data confirm previous findings about the chemotactic responses to platelet derivatives of retinal glial cells [Castelnovo et al, 2000], rat foetal cells [Soffer et al, 2003], mature osteoblasts [Celotti et al, 2006], keratinocytes [Ranzato et al, 2008] and primary fibroblasts [Ranzato et al, 2009b].…”

Section: Discussionsupporting

confidence: 93%

“…Hence, we confirmed the key experiments of this study in human microvascular cells, and also included in the study non-human vascular models, in order to obtain a wider panel of data about the potentialities of PL factors in the stimulation of wound healing. The CV assay highlighted a mitogenic effect of PL, thus confirming previous findings about cell proliferation induced by PL on different cell types [Ranzato et al, 2008[Ranzato et al, , 2009b. Interestingly, the proliferative effect of PL was maximum in microvascular HMVEC-c cells, in which the expression of PDGFR and CXCR3 has been characterised.…”

Section: Discussionsupporting

confidence: 86%

“…It has been also shown that Ca 2þ rises lead to nitric oxide production, which in turn promotes endothelial cell proliferation [Erdogan et al, 2005]. A correlation between Ca 2þ dynamics and wound repair has been also reported in studies on skin cells, where PL has induced noticeable Ca 2þ signals, and BAPTA-AM has abolished the PL effect on wound healing [Ranzato et al, 2008[Ranzato et al, , 2009b. In our experiments, the role of cell Ca 2þ in HuVEC endothelial repair was revealed by the inhibitory effect on PL of the Ca 2þ chelator BAPTA-AM.…”

Section: Discussionmentioning

confidence: 88%

“…The PL was obtained as described by Ranzato et al [2008Ranzato et al [ , 2009b. Briefly, platelet concentrates were derived by platelet aphaeresis collection from blood samples of single volunteer donors after informed consent.…”

Section: Cell Line and Platelet Lysate (Pl) Preparationmentioning

confidence: 99%

“…This platelet derivative, which is obtained from repeated freezingthawing of platelet-enriched blood samples, has been shown to accelerate the in vitro wound healing of skin cells [Ranzato et al, 2008[Ranzato et al, , 2009b and mouse myoblasts [Ranzato et al, 2009a]. It has been also used as a dressing in clinical practice to improve wound healing [Mazzucco et al, 2004].…”

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confidence: 99%

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Role of ERK1/2 in platelet lysate‐driven endothelial cell repair

Ranzato

Boccafoschi

Mazzucco

et al. 2010

J of Cellular Biochemistry

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Mechanisms of endothelial repair induced by a platelet lysate (PL) were studied on human (HuVEC, HMVEC-c) and non-human (PAOEC, bEnd5) endothelial cells. A first set of analyses on these cells showed that 20% (v/v) PL promotes scratch wound healing, with a maximum effect on HuVEC. Further analyses made on HuVEC showed that the ERK inhibitor PD98059 maximally inhibited the PL-induced endothelial repair, followed in order of importance by the calcium chelator BAPTA-AM, the PI3K inhibitor wortmannin and the p38 inhibitor SB203580. The PL exerted a chemotactic effect on HuVEC, which was abolished by all the above inhibitors, and induced a PD98059-sensitive increase of cell proliferation rate. Confocal calcium imaging of fluo-3-loaded HuVEC showed that PL was able to induce cytosolic free Ca(2+) oscillations, visible also in Ca(2+)-free medium, suggesting an involvement of Ins3P-dependent Ca(2+) release. Western blot analysis on scratch wounded HuVEC showed that PL induced no activation of p38, a transient activation of AKT, and a sustained activation of ERK1/2. The complex of data indicates that, although different signalling pathways are involved in PL-promoted endothelial repair, the process is chiefly under the control of ERK1/2.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 88%

Section: Cell Line and Platelet Lysate (Pl) Preparationmentioning

confidence: 99%

mentioning

confidence: 99%

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Role of ERK1/2 in platelet lysate‐driven endothelial cell repair

Ranzato

Boccafoschi

Mazzucco

et al. 2010

J of Cellular Biochemistry

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Local Delivery of Growth Factors Using Wound Dressings

Catanzano

Boateng

2020

Therapeutic Dressings and Wound Healing Applications

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2.1 Background 2.2 Aetiology of Diabetic Foot Ulcers 2.3 Standard of Care for Treatment of Diabetic Foot Ulcers 2.4 Commonly Used Wound Dressings for Diabetic Foot Ulcers and Their Mechanism of Action 2.5 Absorbent and Superabsorbent Dressings 2.6 Alginates 2.7 Films 2.8 Foams 2.9 Honeys 2.10 Hydrogels 2.11 The Role of a Split Thickness Skin Graft in Diabetic Foot Ulcers 2.12 Negative Pressure Wound Therapy 2.13 Larval Therapy 2.14 Clinical Case Studies from Multidisciplinary Diabetic Foot Clinic 2.14.1 Neuropathic Wound 2.14.2 Ischaemic Wound 2.14.3 Neuro-Ischaemic Wound 2.14.4 Osteomyelitis 2.14.5 Charcot's Foot 2.14.6 Necrotising Fasciitis in a Patient with Diabetes 2.15 Summary Acknowledgements References

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Platelet lysate and adipose mesenchymal stromal cells on silk fibroin nonwoven mats for wound healing

Chlapanidas

Perteghella

Faragò

et al. 2015

J of Applied Polymer Sci

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In this work platelet lysate (PL) and adipose‐derived mesenchymal stromal cells (ASCs) seeded on nonwoven fibroin mats were in vitro and in vivo evaluated for tissue regenerative applications. Nonwoven mats obtained by a large scale water entanglement technique were characterized for their physico‐chemical properties. Results indicated a high purity of fibroin fibers, their stability after sterilization process and appropriate technological properties suitable for tissue engineering. Moreover, the scaffolds in vitro supported adhesion and migration of ASCs and the presence of PL improved the cell proliferation. The products were then applied on epithelial/dermal wounds carried out on the dorsal surface of rabbit: the skin reparative process was solved in 9 days, with a completely restitutio ad integrum of the epithelium in animals treated with PL alone; ASCs did not further improve the wound healing. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 42942.

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Platelet lysate stimulates wound repair of HaCaT keratinocytes

Abstract: Data indicate that PL increases wound healing rate by stimulating keratinocyte migration through a calcium- and p38-dependent mechanism. ERK1/2 and phosphoinositide-3 kinase seem to play minor roles.

Cited by 44 publications

References 42 publications

Role of ERK1/2 in platelet lysate‐driven endothelial cell repair

Role of ERK1/2 in platelet lysate‐driven endothelial cell repair

Local Delivery of Growth Factors Using Wound Dressings

Platelet lysate and adipose mesenchymal stromal cells on silk fibroin nonwoven mats for wound healing

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