Platelet lysate-loaded PLGA nanoparticles in a thermo-responsive hydrogel intended for the treatment of wounds

Bernal-Chávez, Sergio Alberto; Alcalá-Alcalá, Sergio; Cerecedo, Doris; Ganem-Rondero, Adriana

doi:10.1016/j.ejps.2020.105231

Cited by 18 publications

(12 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6 Since Zhang's team used erythrocyte membranes wrapped around nanoparticles for the first time to improve the biocompatibility of nanomaterials, 7 researchers have dedicated much effort to find more applications of biomimetic nanomaterials in the treatment of various diseases. [8][9][10][11][12][13][14][15] Various cell types have been developed, including macrophages, [16][17][18] T cells, 19,20 neutrophils, 21,22 stem cells, [23][24][25][26][27] red blood cells, [28][29][30][31][32] and platelets. 33,34 Biomimetic nanomaterials show many advantages, such as high biocompatibility, longer cycle life, inherent biodegradability, natural targeting ability of cells/tissues, high drug loading, and ability to cross biological barriers.…”

Section: Introductionmentioning

confidence: 99%

<p>Platelet-Mimicking Drug Delivery Nanoparticles for Enhanced Chemo-Photothermal Therapy of Breast Cancer</p>

Pei¹,

Huang²,

Chen³

et al. 2020

IJN

View full text Add to dashboard Cite

Background Traditional nanoparticle-based drug delivery systems suffer from several limitations, such as easy clearance from blood and inaccurate targeting. Materials and Methods Here, we developed platelet membrane-coated nanoparticles (PM-NPs) to improve the precise delivery of drugs to tumor sites and enable a more efficient photothermal therapy (PTT) treatment. Results Mimicking the natural platelet membrane, nanoparticles containing drugs and photothermal agents were not recognized and cleared by the immune system; they could circulate in the blood for a long time and accumulate more efficiently at the tumor site, thus releasing more antitumor drugs and achieving better PTT effects. It is worth mentioning that, in this study, we found that tumors in mice treated with the platelet-mimicking nanoparticles were completely eliminated without recurrence during the observation period (up to 18 days). Conclusion This study provides a new strategy to design delivery systems of drugs or photothermal agents, whether in biotherapy or other fields.

show abstract

Section: Introductionmentioning

confidence: 99%

<p>Platelet-Mimicking Drug Delivery Nanoparticles for Enhanced Chemo-Photothermal Therapy of Breast Cancer</p>

Pei¹,

Huang²,

Chen³

et al. 2020

IJN

View full text Add to dashboard Cite

show abstract

“…HPL-loaded CS/DHO lyophilizates were reconstituted to a volume of 3 mL using normal saline and were incubated at 33 °C ± 1 °C for 30 min to ensure complete gelation. 5 mL phosphate buffered saline (PBS) was carefully placed on top of the thermogels then samples were gently shaken at 100 rpm in a shaking incubator (GFL Gesellschaft für Labortechnik, Burgwedel, Germany) (Bernal-Chávez et al., 2020 ). At predetermined intervals, the entire PBS solution was carefully pipetted out of the sample vials without interrupting the gel surface and used for TP and PDGF-BB analyses as detailed below.…”

Section: Methodsmentioning

confidence: 99%

“…TP release was quantified by means of a pre-developed calibration curve for UV-absorbance at λ max of 280 nm (UV-VIS Spectro double beam, Labomed Inc., USA) (Bernal-Chávez et al., 2020 ). The amount of HPL released at each time point was calculated using a pre-set calibration curve.…”

Section: Methodsmentioning

confidence: 99%

Chitosan-dipotassium orthophosphate lyophilizate: a novel in situ thermogel carrier system of allogeneic platelet lysate growth factors

et al. 2022

View full text Add to dashboard Cite

The clinical success of platelet-rich plasma (PRP) is constrained by its limited mechanical strength, rapid disintegration by lytic enzymes, and the consequent short-term release of bioactive growth factors (GFs). Recently, attempts to formulate PRP and other hemoderivatives, such as platelet lysate (PL) have been underway. The current study aimed to formulate allogeneic freeze-dried human platelet lysate (HPL) onto lyophilized chitosan - dipotassium hydrogen orthophosphate (CS/DHO) thermo-sensitive scaffolds. A systemic approach was employed to optimize freeze-drying (FD) procedures targeting predefined critical quality attributes (CQAs). Thermal behavior, vibrational spectroscopy, morphological and moisture content analyses were used to detect possible protein destabilization during formulation and suboptimal cake properties. The effect of CS/DHO concentrations on thermo-responsiveness and release kinetics were investigated. Finally, six-months stability and cytotoxicity studies were carried out. An optimized lyophilizate was attainable with residual moisture of less than 5% and thermoresponsive to 33 °C in less than 3 min. HPL proteins were sustainedly released over five days in a pH-sensitive manner. The stability study indicated plausible physical and biochemical stability. Cell viability testing supported the cytocompatibility of the system. Finally, the lyophilizate variant of CS/DHO thermogel overcomes limited storage stability previously posed as a challenge in freshly prepared thermogels. The developed system overcomes the drawbacks of currently used PRP treatment and provides a novel GF-rich scaffold for wound repair.

show abstract

“…Moreover, their loading capacity, drug release kinetics, and biological performance could be regulated by adjusting their composition or surface charge. However, these NPs are quite predisposed to premature burst release of drugs [ 13 , 22 ], their permanency on the target site until complete drug release is unpredictable [ 13 , 23 ]. When in contact with the biological environment, NPs can present instability or clearance by the immune system [ 5 ].…”

Section: Nps-loaded Hydrogel Systemmentioning

confidence: 99%

“…Bernal-Chávez et al (2020) developed a Pluronic F-127 thermo-responsive hydrogel containing PLGA NPs loaded with a platelet lysate to treat chronic wounds [ 23 ]. The hydrogel formulation was optimized, and the gelation temperature was 32 °C, allowing the formulation to solidify in situ and be retained in the wound area during the platelet lysate release period.…”

Section: Routes Of Drugs Administrationmentioning

confidence: 99%

Polymeric Nanoparticles-Loaded Hydrogels for Biomedical Applications: A Systematic Review on In Vivo Findings

et al. 2022

View full text Add to dashboard Cite

Clinically available medications face several hurdles that limit their therapeutic activity, including restricted access to the target tissues due to biological barriers, low bioavailability, and poor pharmacokinetic properties. Drug delivery systems (DDS), such as nanoparticles (NPs) and hydrogels, have been widely employed to address these issues. Furthermore, the DDS improves drugs’ therapeutic efficacy while reducing undesired side effects caused by the unspecific distribution over the different tissues. The integration of NPs into hydrogels has emerged to improve their performance when compared with each DDS individually. The combination of both DDS enhances the ability to deliver drugs in a localized and targeted manner, paired with a controlled and sustained drug release, resulting in increased drug therapeutic effectiveness. With the incorporation of the NPs into hydrogels, it is possible to apply the DDS locally and then provide a sustained release of the NPs in the site of action, allowing the drug uptake in the required location. Additionally, most of the materials used to produce the hydrogels and NPs present low toxicity. This article provides a systematic review of the polymeric NPs-loaded hydrogels developed for various biomedical applications, focusing on studies that present in vivo data.

show abstract

Platelet lysate-loaded PLGA nanoparticles in a thermo-responsive hydrogel intended for the treatment of wounds

Cited by 18 publications

References 49 publications

<p>Platelet-Mimicking Drug Delivery Nanoparticles for Enhanced Chemo-Photothermal Therapy of Breast Cancer</p>

<p>Platelet-Mimicking Drug Delivery Nanoparticles for Enhanced Chemo-Photothermal Therapy of Breast Cancer</p>

Chitosan-dipotassium orthophosphate lyophilizate: a novel in situ thermogel carrier system of allogeneic platelet lysate growth factors

Polymeric Nanoparticles-Loaded Hydrogels for Biomedical Applications: A Systematic Review on In Vivo Findings

Contact Info

Product

Resources

About