Platelet lysate loaded electrospun scaffolds: Effect of nanofiber types on wound healing

Cordenonsi, Leticia Malgarim; Faccendini, Angela; Rossi, Silvia; Malavasi, Lorenzo; Raffin, Renata Platcheck; Schapoval, Elfrides Eva Scherman; Fante, Claudia Del; Vigani, Barbara; Miele, Dalila; Ferrari, Franca

doi:10.1016/j.ejpb.2019.06.030

Cited by 31 publications

(9 citation statements)

References 28 publications

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“…Wound healing is a procedure in which the cells can regenerate and treat the injured tissue [2]. This process occurs across four continuous and partially overlapping phases, consisting of hemostasis, inflammation surrounding the injured region, cellular migration and proliferation, and ECM remodeling ( Figure 1) [40][41][42].…”

Section: Wound Healingmentioning

confidence: 99%

Bio-Based Electrospun Fibers for Wound Healing

Azimi

Maleki

Zavagna

et al. 2020

JFB

129

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Being designated to protect other tissues, skin is the first and largest human body organ to be injured and for this reason, it is accredited with a high capacity for self-repairing. However, in the case of profound lesions or large surface loss, the natural wound healing process may be ineffective or insufficient, leading to detrimental and painful conditions that require repair adjuvants and tissue substitutes. In addition to the conventional wound care options, biodegradable polymers, both synthetic and biologic origin, are gaining increased importance for their high biocompatibility, biodegradation, and bioactive properties, such as antimicrobial, immunomodulatory, cell proliferative, and angiogenic. To create a microenvironment suitable for the healing process, a key property is the ability of a polymer to be spun into submicrometric fibers (e.g., via electrospinning), since they mimic the fibrous extracellular matrix and can support neo- tissue growth. A number of biodegradable polymers used in the biomedical sector comply with the definition of bio-based polymers (known also as biopolymers), which are recently being used in other industrial sectors for reducing the material and energy impact on the environment, as they are derived from renewable biological resources. In this review, after a description of the fundamental concepts of wound healing, with emphasis on advanced wound dressings, the recent developments of bio-based natural and synthetic electrospun structures for efficient wound healing applications are highlighted and discussed. This review aims to improve awareness on the use of bio-based polymers in medical devices.

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Section: Wound Healingmentioning

confidence: 99%

Bio-Based Electrospun Fibers for Wound Healing

Azimi

Maleki

Zavagna

et al. 2020

JFB

129

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“…Mechanical properties were assessed using a texture analyzer (TA-XT plus, Stable Microsystems, Enco, Spinea, Italy) equipped with a 1 kg load cell and A/TG tensile grips [19]. Rectangular portions (3 × 1 cm) of each scaffold (thickness~100 µm) were kept vertical by means of two grips, the lower one fixed and the upper one movable at a constant rate of 0.5 mm/s.…”

Section: Mechanical Propertiesmentioning

confidence: 99%

Norfloxacin-Loaded Electrospun Scaffolds: Montmorillonite Nanocomposite vs. Free Drug

et al. 2020

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Infections in nonhealing wounds remain one of the major challenges. Recently, nanomedicine approach seems a valid option to overcome the antibiotic resistance mechanisms. The aim of this study was the development of three types of polysaccharide-based scaffolds (chitosan-based (CH), chitosan/chondroitin sulfate-based (CH/CS), chitosan/hyaluronic acid-based (CH/HA)), as dermal substitutes, to be loaded with norfloxacin, intended for the treatment of infected wounds. The scaffolds have been loaded with norfloxacin as a free drug (N scaffolds) or in montmorillonite nanocomposite (H—hybrid-scaffolds). Chitosan/glycosaminoglycan (chondroitin sulfate or hyaluronic acid) scaffolds were prepared by means of electrospinning with a simple, one-step process. The scaffolds were characterized by 500 nm diameter fibers with homogeneous structures when norfloxacin was loaded as a free drug. On the contrary, the presence of nanocomposite caused a certain degree of surface roughness, with fibers having 1000 nm diameters. The presence of norfloxacin–montmorillonite nanocomposite (1%) caused higher deformability (90–120%) and lower elasticity (5–10 mN/cm2), decreasing the mechanical resistance of the systems. All the scaffolds were proven to be degraded via lysozyme (this should ensure scaffold resorption) and this sustained the drug release (from 50% to 100% in 3 days, depending on system composition), especially when the drug was loaded in the scaffolds as a nanocomposite. Moreover, the scaffolds were able to decrease the bioburden at least 100-fold, proving that drug loading in the scaffolds did not impair the antimicrobial activity of norfloxacin. Chondroitin sulfate and montmorillonite in the scaffolds are proven to possess a synergic performance, enhancing the fibroblast proliferation without impairing norfloxacin’s antimicrobial properties. The scaffold based on chondroitin sulfate, containing 1% norfloxacin in the nanocomposite, demonstrated adequate stiffness to sustain fibroblast proliferation and the capability to sustain antimicrobial properties to prevent/treat nonhealing wound infection during the healing process.

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“…where L break is the distance of the two grips at scaffold breaking and L 0 is the initial distance of the two grips. Moreover, Young's Modulus (mN/cm 2 ) was calculated as the slope of the initial linear portion of force vs. grip displacement [10,18,19].…”

Section: Mechanical Propertiesmentioning

confidence: 99%

Halloysite- and Montmorillonite-Loaded Scaffolds as Enhancers of Chronic Wound Healing

et al. 2020

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The increase in life expectancy and the increasing prevalence of diabetic disease and venous insufficiency lead to the increase of chronic wounds. The prevalence of ulcers ranges from 1% in the adult population to 3–5% in the over 65 years population, with 3–5.5% of the total healthcare expenditure, as recently estimated. The aim of this work was the design and the development of electrospun scaffolds, entirely based on biopolymers, loaded with montmorillonite (MMT) or halloysite (HNT) and intended for skin reparation and regeneration, as a 3D substrate mimicking the dermal ECM. The scaffolds were manufactured by means of electrospinning and were characterized for their chemico-physical and preclinical properties. The scaffolds proved to possess the capability to enhance fibroblast cells attachment and proliferation with negligible proinflammatory activity. The capability to facilitate the cell adhesion is probably due to their unique 3D structure which are assisting cell homing and would facilitate wound healing in vivo.

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Platelet lysate loaded electrospun scaffolds: Effect of nanofiber types on wound healing

Cited by 31 publications

References 28 publications

Bio-Based Electrospun Fibers for Wound Healing

Bio-Based Electrospun Fibers for Wound Healing

Norfloxacin-Loaded Electrospun Scaffolds: Montmorillonite Nanocomposite vs. Free Drug

Halloysite- and Montmorillonite-Loaded Scaffolds as Enhancers of Chronic Wound Healing

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