Platelet lysate induces chondrogenic differentiation of umbilical cord‐derived mesenchymal stem cells by regulating the lncRNA H19/miR‐29b‐3p/SOX9 axis

Cao, Boran; Dai, Xin

doi:10.1002/2211-5463.13002

Cited by 8 publications

(4 citation statements)

References 35 publications

(58 reference statements)

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“…Its overexpression resulted in increased ALP (Alkaline Phosphatase) activity and higher expression of the osteogenic markers [ 35 ]. Similarly, chondrogenic differentiation of human umbilical cord MSCs was promoted by the overexpression of H19 [ 36 ], while the adipogenic differentiation of BM-MSCs and adipose tissue-derived MSCs (ADSCs) was inhibited [ 37 , 38 ]. The current results reveal that H19 is involved in osteogenic, chondrogenic and adipogenic differentiation of WJ-MSCs.…”

Section: Discussionmentioning

confidence: 99%

Expression Profile of New Marker Genes Involved in Differentiation of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells into Chondrocytes, Osteoblasts, Adipocytes and Neural-like Cells

Stefańska,

Nemcova,

Blatkiewicz

et al. 2023

IJMS

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Wharton’s jelly (WJ) contains mesenchymal stem cells (MSCs) exhibiting broad immunomodulatory properties and differentiation capacity, which makes them a promising tool for cellular therapies. Although the osteogenic, chondrogenic and adipogenic differentiation is a gold standard for proper identification of MSCs, it is important to elucidate the exact molecular mechanisms governing these processes to develop safe and efficient cellular therapies. Umbilical cords were collected from healthy, full-term deliveries, for subsequent MSCs (WJ-MSCs) isolation. WJ-MSCs were cultivated in vitro for osteogenic, chondrogenic, adipogenic and neurogenic differentiation. The RNA samples were isolated and the transcript levels were evaluated using NovaSeq platform, which led to the identification of differentially expressed genes. Expression of H19 and SLPI was enhanced in adipocytes, chondrocytes and osteoblasts, and NPPB was decreased in all analyzed groups compared to the control. KISS1 was down-regulated in adipocytes, chondrocytes, and neural-like cells compared to the control. The most of identified genes were already implicated in differentiation of MSCs; however, some genes (PROK1, OCA2) have not yet been associated with initiating final cell fate. The current results indicate that both osteo- and adipo-induced WJ-MSCs share many similarities regarding the most overexpressed genes, while the neuro-induced WJ-MSCs are quite distinctive from the other three groups. Overall, this study provides an insight into the transcriptomic changes occurring during the differentiation of WJ-MSCs and enables the identification of novel markers involved in this process, which may serve as a reference for further research exploring the role of these genes in physiology of WJ-MSCs and in regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

Expression Profile of New Marker Genes Involved in Differentiation of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells into Chondrocytes, Osteoblasts, Adipocytes and Neural-like Cells

Stefańska,

Nemcova,

Blatkiewicz

et al. 2023

IJMS

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“…This variability may be attributed to the diverse range of patient demographics, including age and gender. miR-29 family members, including miR-29a, miR-29b, and miR-29c, have been implicated in the regulation of ECM proteins, such as collagen, which are important for bone development and homeostasis [52][53][54][55][56]. The aberrant regulation of those miRNAs in patients with OI type I induced by mutations in COL1A1 may contribute to reduced collagen synthesis and impaired bone formation.…”

Section: Discussionmentioning

confidence: 99%

Analysis of miRNAs in Osteogenesis imperfecta Caused by Mutations in COL1A1 and COL1A2: Insights into Molecular Mechanisms and Potential Therapeutic Targets

Botor,

Auguściak-Duma,

Lesiak

et al. 2023

Pharmaceuticals

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Osteogenesis imperfecta (OI) is a group of connective tissue disorders leading to abnormal bone formation, mainly due to mutations in genes encoding collagen type I (Col I). Osteogenesis is regulated by a number of molecules, including microRNAs (miRNAs), indicating their potential as targets for OI therapy. The goal of this study was to identify and analyze the expression profiles of miRNAs involved in bone extracellular matrix (ECM) regulation in patients diagnosed with OI type I caused by mutations in COL1A1 or COL1A2. Primary skin fibroblast cultures were used for DNA purification and sequence analysis, followed by analysis of miRNA expression. Sequencing analysis revealed mutations of the COL1A1 or COL1A2 genes in all OI patients, including four previously unreported. Amongst the 40 miRNAs analyzed, 9 were identified exclusively in OI cells and 26 in both OI patients and the controls. In the latter case, the expression of six miRNAs (hsa-miR-10b-5p, hsa-miR-19a-3p, hsa-miR-19b-3p, has-miR-204-5p, has-miR-216a-5p, and hsa-miR-449a) increased, while four (hsa-miR-129-5p, hsa-miR-199b-5p, hsa-miR-664a-5p, and hsa-miR-30a-5p) decreased significantly in OI cells in comparison to their expression in the control cells. The identified mutations and miRNA expression profiles shed light on the intricate processes governing bone formation and ECM regulation, paving the way for further research and potential therapeutic advancements in OI and other genetic diseases related to bone abnormality management.

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“…LncRNA H19 is well-known in tumorigenesis as a tumor suppressor [17], and recently, the role of H19 in other pathologies has been noticed, including atherosclerosis [18], osteoporosis [19], fibrosis [20], and ischemic stroke [21]. lncRNA H19 acts as a metabolic correlate in cartilage and cultured chondrocytes [22], and the overexpression of H19 could promote the chondrogenic differentiation of hUCMSCs [23]. Along with the clue from the screening of H19 in E7-KGMs via RNA-seq, we validated the significantly higher expression of H19 in the E7-KGM group compared to the RGD-KGM and EC-KGM groups.…”

Section: Discussionmentioning

confidence: 99%

E7 Peptide Enables BMSC Adhesion and Promotes Chondrogenic Differentiation of BMSCs Via the LncRNA H19/miR675 Axis

Shi

et al. 2023

Bioengineering

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Therapeutic strategies based on utilizing endogenous BMSCs have been developed for the regeneration of bone, cartilage, and ligaments. We previously found that E7 peptide (EPLQLKM) could enhance BMSC homing in bio-scaffolds and, therefore, promote cartilage regeneration. However, the profile and mechanisms of E7 peptide in cartilage regeneration remain elusive. In this study, we examined the effect of E7 peptide on the BMSC phenotype, including adhesion, viability and chondrogenic differentiation, and its underlying mechanism. The konjac glucomannan microsphere (KGM), a carrier material that is free of BMSC adhesion ability, was used as the solid base of E7 peptide to better explore the independent role of E7 peptide in BMSC behavior. The results showed that E7 peptide could support BMSC adhesion and viability in a comparable manner to RGD and promote superior chondrogenic differentiation to RGD. We examined differentially expressed genes of BMSCs induced by E7 compared to RGD. Subsequently, a real-time PCR validated the significantly upregulated expression of lncRNA H19, and the knockdown of lncRNA H19 or miR675, a downstream functional unit of H19, could significantly obscure the chondrogenic differentiation induced by E7. In conclusion, this study confirmed the independent role of E7 in the adhesion and viability of BMSCs and revealed the pro-chondrogenic effect of E7 on BMSCs via the H19/miR675 axis. These results could help establish new therapeutic strategies based on employing endogenous BMSCs for cartilage tissue regeneration.

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Platelet lysate induces chondrogenic differentiation of umbilical cord‐derived mesenchymal stem cells by regulating the lncRNA H19/miR‐29b‐3p/SOX9 axis

Cited by 8 publications

References 35 publications

Expression Profile of New Marker Genes Involved in Differentiation of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells into Chondrocytes, Osteoblasts, Adipocytes and Neural-like Cells

Expression Profile of New Marker Genes Involved in Differentiation of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells into Chondrocytes, Osteoblasts, Adipocytes and Neural-like Cells

Analysis of miRNAs in Osteogenesis imperfecta Caused by Mutations in COL1A1 and COL1A2: Insights into Molecular Mechanisms and Potential Therapeutic Targets

E7 Peptide Enables BMSC Adhesion and Promotes Chondrogenic Differentiation of BMSCs Via the LncRNA H19/miR675 Axis

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