Platelet-derived Growth Factor Stimulation of Monocyte Chemoattractant Protein-1 Gene Expression Is Mediated by Transient Activation of the Phosphoinositide 3-Kinase Signal Transduction Pathway

TNF-α Stimulation of MCP-1 Expression Is Mediated by the Akt/PKB Signal Transduction Pathway in Vascular Endothelial Cells

“…In contrast, wortmannin failed to inhibit the IL-1␤ induction of MCP-1 expression in HUVECs, thus suggesting a different pathway for the activity of this growth factor. Similar result is observed that the inability of wortmannin to inhibit IL-1␤-mediated MCP-1 expression in fibroblasts (22). Although IL-1␤ has been shown to activate NF-B and AP-1 in human epidermoid carcinoma cells and hepatoma HepG2 cells via a PI3-kinase-mediated pathway (23), further studies will be necessary to identify the signal transduction mechanism of each cytokine.…”

“…In other studies, results suggested that platelet-derived growth factor (PDGF), one of the growth factors that activate PI3-kinase is a necessary and sufficient event for the induction of the MCP-1 mRNA in fibroblasts. Additional results showed that PDGF-stimulated expression of MCP-1 may involve Akt/PKB (22). These findings prompted us to examine the role of Atk in mediating the actions of TNF-␣-induced MCP-1 expression.…”

Neurotensin receptor‐3/sortilin mediates neurotensin‐induced cytokine/chemokine expression in a murine microglial cell line

“…Previous studies have reported that MAP kinases that activated expression of transcription factors including AP‐1, CREB, ATF‐1, and NF‐κB, also regulated production of cytokines IL‐1β and TNF‐α in LPS‐ or β‐amyloid activated microglia (Pyo et al, 1998; Combs et al, 1999). Activation of the PI‐3 kinase and Erk pathways has been shown in numerous paradigms to be necessary for induction of MCP‐1 (Alberta et al, 1999; Fietta et al, 2002) and MIP‐2 gene expression (Kurosaka et al, 2003; Ojanieme et al, 2003). Similar to our observation, however, it was shown previously in staurosporine‐induced rat peritoneal neutrophils that the Erk/MAPK pathway was required for MIP‐2 protein production at the posttranscriptional but not the transcriptional level (Xiao et al, 1999).…”

PDGF Synergistically Enhances IFN-γ-Induced Expression of CXCL10 in Blood-Derived Macrophages: Implications for HIV Dementia

“…The PI3K pathway plays a critical role in PDGF signaling upstream of PDK1/2 and Akt/PKB kinases (39). To dissect the role of this pathway in the IFN-␥ and PDGF-mediated synergy of CXCL10 expression, cell lysates were examined for the phosphorylation of PDK1 and Akt.…”