Platelet-derived growth factor receptor alpha-positive cells: a new cell type in the human ureteropelvic junction

Hunziker, Manuela; O’Donnell, Anne-Marie; Puri, Prem

doi:10.1038/pr.2017.193

Cited by 10 publications

(9 citation statements)

References 27 publications

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“…Although we observed only low expression of Kcnn3 , expression of SK3 protein was reported in PDGFRα + cells in human renal UPJ (Hunziker et al . 2017), and SK3 expression (but not PDGFRα expression) was reduced in patients with uretero‐pelvic junction obstruction (Hunziker et al . 2017).…”

Section: Discussionmentioning

confidence: 99%

“…2017), and SK3 expression (but not PDGFRα expression) was reduced in patients with uretero‐pelvic junction obstruction (Hunziker et al . 2017). Difference in the expression of SK3 in PDGFRα + cells may be species‐dependent.…”

Section: Discussionmentioning

confidence: 99%

“…Although previous studies have reported PDGFRα + cells in the human ureteropelvic junction (UPJ) (Hunziker et al . 2017) and in the mouse renal pelvis (Hashitani et al . 2017), detailed localization, channel expression and function of PDGFRα + cells is lacking.…”

Section: Introductionmentioning

confidence: 99%

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Identification and classification of interstitial cells in the mouse renal pelvis

Grainger

Freeman

Shonnard

et al. 2020

The Journal of Physiology

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Platelet-derived growth factor receptor-α (PDGFRα) is a novel biomarker along with smooth myosin heavy chain for the pacemaker cells (previously termed 'atypical' smooth muscle cells) in the murine and cynomolgus monkey pelvis-kidney junction. r PDGFRα + cells present in adventitial and urothelial layers of murine renal pelvis do not express smooth muscle myosin heavy chain (smMHC) but are in close apposition to nerve fibres. r Most c-Kit + cells in the renal pelvis are mast cells. Mast cells (CD117 + /CD45 +) are more abundant in the proximal renal pelvis and pelvis-kidney junction regions whereas c-Kit + interstitial cells (CD117 + /CD45 −) are found predominantly in the distal renal pelvis and ureteropelvic junction. r PDGFRα + cells are distinct from c-Kit + interstitial cells. r A subset of PDGFRα + cells express the Ca 2+-activated Cl − channel, anoctamin-1, across the entire renal pelvis. r Spontaneous Ca 2+ transients were observed in c-Kit + interstitial cells, smMHC + PDGFRα cells and smMHC − PDGFRα cells using mice expressing genetically encoded Ca 2+ sensors.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Identification and classification of interstitial cells in the mouse renal pelvis

Grainger

Freeman

Shonnard

et al. 2020

The Journal of Physiology

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“…The known network of peptide ligands and GPCRs spans 407 interactions between 219 peptides and 138 receptors in human [ 166 ]. For example, growth factors are recognized by specialized receptor tyrosine kinases, such as epidermal growth factor receptors (EGFRs) [ 167 ] and platelet-derived growth factor receptor alpha (PDGFRα) [ 168 ]. The endothelin signaling peptides bind to their respective endothelin receptors ETA, ETB1, ETB2 and ETC [ 163 ].…”

Section: Biological Function Of Different Peptide Groupsmentioning

confidence: 99%

Peptidome: Chaos or Inevitability

Lyapina

Иванов

Fesenko

2021

IJMS

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Thousands of naturally occurring peptides differing in their origin, abundance and possible functions have been identified in the tissue and biological fluids of vertebrates, insects, fungi, plants and bacteria. These peptide pools are referred to as intracellular or extracellular peptidomes, and besides a small proportion of well-characterized peptide hormones and defense peptides, are poorly characterized. However, a growing body of evidence suggests that unknown bioactive peptides are hidden in the peptidomes of different organisms. In this review, we present a comprehensive overview of the mechanisms of generation and properties of peptidomes across different organisms. Based on their origin, we propose three large peptide groups—functional protein “degradome”, small open reading frame (smORF)-encoded peptides (smORFome) and specific precursor-derived peptides. The composition of peptide pools identified by mass-spectrometry analysis in human cells, plants, yeast and bacteria is compared and discussed. The functions of different peptide groups, for example the role of the “degradome” in promoting defense signaling, are also considered.

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“…Besides the bladder, PDGFRα (+) cells are distributed in other regions of the urinary tract including the renal pelvis, 8 ureteropelvic junction, 9 and urethra 10 where various forms of spontaneous activity are generated in their smooth muscles. PDGFRα mRNA is widely expressed in the male reproductive tissues 11 including the seminal vesicle where PDGFRα (+) cells are distributed in the mucosa.…”

Section: Introductionmentioning

confidence: 99%

Functional heterogeneity of PDGFRα (+) cells in spontaneously active urogenital tissues

Hashitani

Mitsui

Lang

2020

Neurourology and Urodynamics

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Aims As PDGFRα (+) cells appear not to suppress the excitability of detrusor smooth muscle by generating SK3‐dependent hyperpolarising as proposed in the gastrointestinal tract, we further explored the functional roles of PDGFRα (+) cells in regulating the spontaneous activity of urogenital tissues. Methods Using PDGFRα‐eGFP mice, intracellular Ca2+ signaling in PDGFRα (+) cells of the bladder lamina propria, renal pelvis, and seminal vesicle were visualized using Cal‐590 fluorescence. The distribution and SK3 expression of PDGFRα (+) cells were also examined by immunohistochemistry. Results In the bladder lamina propria, SK3 (−) PDGFRα (+) cells exhibited spontaneous Ca2+ transients and responded to stimulation of P2Y1 purinoceptors with MRS2365 (100 nM) or adenosine diphosphate (ADP) (100 μM) by developing Ca2+ transients. In the proximal renal pelvis, PDGFRα (+) cells were distributed in the mucosal, muscular and serosal layers but did not express SK3 immunoreactivity. PDGFRα (+) cells in the musculature resembling atypical smooth muscle cells generated spontaneous Ca2+ transients that were partially suppressed upon P2Y1‐stimulation, while vigorously responding to human angiotensin II (100 nM). In the seminal vesicle, PDGFRα (+) cells in the musculature but not mucosa expressed SK3 immunoreactivity. In the mucosa, the P2Y1 stimulation evoked Ca2+ transients in both PDGFRα (+) cells and PDGFRα (−) cells. Conclusion PDGFRα (+) cells in spontaneously active urogenital tissues display heterogeneity in terms of their SK3 expression and P2Y1‐induced Ca2+ responses. Muscular PDGFRα (+) cells in the renal pelvis and mucosal PDGFRα (+) cells in the seminal vesicle may generate depolarizing signals to drive smooth muscle cells.

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Platelet-derived growth factor receptor alpha-positive cells: a new cell type in the human ureteropelvic junction

Cited by 10 publications

References 27 publications

Identification and classification of interstitial cells in the mouse renal pelvis

Identification and classification of interstitial cells in the mouse renal pelvis

Peptidome: Chaos or Inevitability

Functional heterogeneity of PDGFRα (+) cells in spontaneously active urogenital tissues

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