Platelet-derived growth factor promotes proliferation of erythropoietic progenitor cells in vitro.

Abstract

A B S T R A C T To investigate serum requirements for optimal erythropoiesis in vitro, we studied the response of erythroid progenitor cell proliferation in culture to platelet-derived growth factor (PDGF). Human bone marrow cells cultured with platelet-poor plasma-derived serum (PDS) form fewer erythroid colonies than do cells cultured with human whole blood serum or fetal calf serum (P < 0.05). Treatment of washed platelets with thrombin releases a low molecular weight (<100,000) factor that enhances colony growth. This secreted factor appears to be PDGF, based upon the ability of partially purified and electrophoretically pure PDGF to restore colony-forming capacity of PDScontaining cultures to 70-96% of the level found in control cultures with whole blood serum or fetal calf serum. Enhancement of colony growth by PDGF was noted only in marrow cultures supplemented with erythropoietin and PDS. Presence of bioactive erythropoietin in PDGF preparations was excluded by assay in hypertransfused, polycythemic mice, and in fasted rats. Although PDGF stimulates erythroid burst formation in marrow cultures containing optimal concentrations of burst-promoting activity (BPA), it does not influence proliferation of circulating erythroid bursts, regardless of BPA concentration added to culture. We conclude that PDGF is a serum determinant of optimal erythroid progenitor cell proliferation in marrow culture. The activity of PDGF is distinct from that of the apparent erythroid specific growth factors erythropoietin and BPA.A preliminary report of this research was presented at the 23rd Annual Meeting of the American Society of Hematology, San Antonio, TX, and published in abstract form in 1981. Blood. 58: 95a.

Platelet-derived growth factor enhances ex vivo expansion of megakaryocytic progenitors from human cord blood

“…PDGF also promotes in vitro multipotent haematopoietic progenitors and erythropoiesis. 23,24 In previous studies, we demonstrated the presence of functional PDGF receptors on human MK and MK cell lines 25 and showed that PDGF promoted MK colony formation. 26 In this study, we aimed to establish a cytokine combination for the efficient ex vivo expansion of the MK lineage from umbilical cord blood CD34 + cells, and to investigate the effect of PDGF in this system.…”

Tissue inhibitor of metalloproteinase‐2 (TIMP‐2) has erythroid‐potentiating activity

“…These CSFs include interleukin-3 (IL-3), granulocyte-macrophage CSF (GM-CSF) and granulocyte CSF (G-CSF) [6][7][8]. Also, platelet-derived growth factor (PDGF), activin, fibroblast growth factor (FGF) growth hormone, insulin and insulin-like growth factor-1 (IGF-1) augment erythropoiesis in vitro [9][10][11][12][13][14][15]. We identified an erythroid-potentiating activity (EPA) in the supernatant of the HTLV-II-transformed human T-lymphblast cell line (Me), and purified the 28 000 Da glycoprotein molecule to homogeneity [5,16].…”

Platelet-derived growth factor enhances in vitro erythropoiesis via stimulation of mesenchymal cells.

“…Recently, Dainiak et al (1) reported that factors released from thrombin-treated platelets and the purified platelet mitogen, PDGF, enhanced human erythropoiesis in vitro.…”

“…1 with an inverted microscope. Aggregates of eight or more hemoglobinized cells were scored as CFU-E-derived colonies.…”

“…Recently it has been reported that platelet-derived growth factor (PDGF)' enhances erythropoiesis when added to cultures of human marrow cells (1). Since no specific PDGF receptor Dr. Delwiche was the recipient of a Scientific Medical Research Foundation Award from the Fonds de la Recherche Scientifique Medicale, Brussels, Belgium.…”