Platelet-derived growth factor binds specifically to receptors on vascular smooth muscle cells and the binding becomes nondissociable.

Williams, Lewis T.; Tremble, Patrice; Antoniades, Harry N.

doi:10.1073/pnas.79.19.5867

Cited by 85 publications

(25 citation statements)

References 18 publications

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“…5-HT not only interacts synergistically with platelet-derived growth factor 4 but also has a direct mitogenic effect on cultured aortic VSMCs. [3][4][5]25,26 It has also been reported that 5-HT stimulates the expression of thrombin receptors in VSMCs probably by 5-HT 2 receptors, which would further potentiate the contraction and proliferation of VSMCs by thrombin. 27 These effects could cause narrowing of the vessel lumen and contribute to both the development of atherosclerosis and the restenosis observed after angioplasty.…”

Section: Discussionmentioning

confidence: 97%

Serotonin induces the expression of tissue factor and plasminogen activator inhibitor-1 in cultured rat aortic endothelial cells

Kawano

Tsuji

Nishimura

et al. 2001

Blood

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Section: Discussionmentioning

confidence: 97%

Serotonin induces the expression of tissue factor and plasminogen activator inhibitor-1 in cultured rat aortic endothelial cells

Kawano

Tsuji

Nishimura

et al. 2001

Blood

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“…The mitogenic effect of PDGF on fibroblasts is initiated by its interaction with a specific 165,000-D receptor, which has been labeled by affinity cross-linking of '25I-PDGF to the cell membranes of 3T3 fibroblasts (2). Reduction of native PDGF yields at least two polypeptides that are not capable of stimulating DNA synthesis (3,4).…”

Section: Introductionmentioning

confidence: 99%

Platelet-derived growth factor stimulates mouse 3T3 cell mitogenesis and leukocyte chemotaxis through different structural determinants.

Williams¹,

Antoniades²,

Goetzl³

1983

J. Clin. Invest.

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“…The possibility that cell-derived growth factors are involved in the initiation and progression of the myointimal lesions, which are characteristic of atherosclerosis, has received particular attention. Recent work has focused on platelet-derived growth factor (PDGF), a potent mitogen for vascular smooth muscle cells in vitro (4,5) that is thought to be released from the a granules ofplatelets adhering to exposed subendothelial connective tissue or foam cells (2,3). Prior to the onset of DNA synthesis, PDGF elicits a variety of responses in aortic smooth muscle cells, including increased prostacyclin biosynthesis (6).…”

mentioning

confidence: 99%

Stimulation of aortic smooth muscle cell mitogenesis by serotonin.

Nemecek¹,

Coughlin²,

Handley³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

293

126

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Bovine aortic smooth muscle cells in vitro responded to 1 nM to 10 ,M serotonin with increased incorporation of [3H]thymidine into DNA. The mitogenic effect of serotonin was half-maximal at 80 nM and maximal above 1 ,uM. At a concentration of 1 ,uM, serotonin stimulated smooth muscle cell mitogenesis to the same extent as human plateletderived growth factor (PDGF) at 12 ng/ml, Tryptamine was =1/10th as potent as serotonin as a mitogen for smooth muscle cells. Other indoles that are structurally related to serotonin (Dand L-tryptophan, 5-hydroxy-L-tryptophan, N-acetyl-5-hydroxytryptamine, melatonin, 5-hydroxyindoleacetic acid, and 5-hydroxytryptophol) and quipazine were inactive. The stimulatory effect of serotonin on smooth muscle cell DNA synthesis required prolonged (20-24 hr) [3HlThymidine Incorporation. Bovine aortic smooth muscle cells from frozen stock (passages 3-12) were suspended in Dulbecco's modified Eagle's medium (DMEM) (pH 7.4) supplemented with 10% calf serum/6 mM glutamine/ penicillin (100 units/ml)/streptomycin (100 pug/ml) for seeding into 16-mm 96-well microtiter plates at a density of w50,000 cells per well. After 48 hr, the growth medium was replaced with serum-free quiescing medium consisting of DMEM and Ham's F12 (1:1; pH 7.4) supplemented with ovalbumin (1 mg/ml)/ascorbic acid (25 ,ug/ml)/selenium (25 ng/ml)/dialyzed Cohn fraction IV (10 ug/ml)/epidermal growth factor (10 ng/ml)/transferrin 10 ,tg/ml) 50 ,uM hydrocortisone/6 mM glutamine/penicillin (100 units/ml)/ streptomycin (100 ,ug/ml) (8). Smooth muscle cells were incubated in the quiescing medium for 96 hr. The medium was changed once during this period. Compounds to be tested were added to smooth muscle cells in quiescing medium 1 hr before serotonin and/or PDGF. The cultures were exposed to serotonin and/or PDGF for 20 hr before [3H]thymidine (2.0 ,uCi/ml; 1 Ci = 37 GBq) was included in the quiescing medium. Four hours after the addition of [3H]thymidine, experiments were terminated by aspirating the medium and subjecting the cultures to sequential washes with Dulbecco's phosphate-buffered saline (pH 7.4) containing 1 mM CaCl2, 1 mM MgCl2, 10% trichloroacetic acid, and ethanol/ether (2:1). Phase-contrast microscopy was used to inspect the microwells for evidence of cell detachment or changes in cell morphology. Acid-insoluble [3H]thymidine was extracted into 0.5 M NaOH and quantified by using a liquid scintillation counter.Materials. Serotonin, tryptamine, L-tryptophan, Dtryptophan, N-acetyl-5-hydroxytryptamine, 5-hydroxy-Ltryptophan, melatonin, 5-hydroxyindoleacetic acid, 5-hydroxytryptophol, propranolol, atropine, cimetidine, 1-methyl-3-isobutylxanthine, ovalbumin, and Cohn fraction IV were obtained from Sigma; quipazine (2-[1-piperazi- 674The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

show abstract

Platelet-derived growth factor binds specifically to receptors on vascular smooth muscle cells and the binding becomes nondissociable.

Abstract: Radioiodinated platelet-derived growth factor

Cited by 85 publications

References 18 publications

Serotonin induces the expression of tissue factor and plasminogen activator inhibitor-1 in cultured rat aortic endothelial cells

Serotonin induces the expression of tissue factor and plasminogen activator inhibitor-1 in cultured rat aortic endothelial cells

Platelet-derived growth factor stimulates mouse 3T3 cell mitogenesis and leukocyte chemotaxis through different structural determinants.

Stimulation of aortic smooth muscle cell mitogenesis by serotonin.

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