Platelet Counts with the Coulter Counter

Elevation of cytokines associated with the thrombocytopenia of equine infectious anaemia.

“…Platelet counts were performed within 90 min of blood collection. Platelet counts for the first nine foals were performed on platelet-rich plasma obtained from sodium citrate-collected blood, counted on a particle counter (Coulter Model ZBI-10) and the plateletrich plasma values corrected to whole blood (Bull et al, 1965). Random samples were also counted in parallel on an automated cell counter (System 9000, Serono-Baker Diagnostics).…”

Comparison of platelet counts by CellDyn Sapphire (Abbot Diagnostics), LH750 (Beckman Coulter), ReaPanThrombo immunoplatelet method (ReaMetrix), and the international flow reference method, in thrombocytopenic blood samples

“…Fifty microliters of whole blood sample was added and after a gentle vortex each tube was incubated for 30 min at room temperature away from direct light exposure. Four hundred fifty microliters of NH 4 Cl lyse solution was added to each tube and vortexed followed by a second incubation step for 15 min at room temperature. The tubes were then analyzed on the FACSCalibur.…”

“…The introduction of automated full blood counters using impedance technology resulted in a dramatic improvement in precision, with typical CVs of <3%, because much higher total numbers of platelets are counted (3,4). However, impedance platelet counting methods have significant limitations, despite their widespread use.…”

Interactions of the Classical and Alternate Complement Pathway with Endotoxin Lipopolysaccharide EFFECT ON PLATELETS AND BLOOD COAGULATION

“…Pooled Wlhite blood cell and platelet determiniations. WVhite blood cell (WBC) and platelet counts were made on 1 ml EDTA anticoagulated retro-orbital blood samples using an electronic particle counter (Coulter Electronics, Inc., Hialeah, Fla.) and standard techniques (19,20). 100 cell differential leukocyte counts were done on air-dried, Wright's stained smears.…”