Platelet count estimation using the CellaVision DM96 system

Abstract

Introduction:Rapid and accurate determination of platelet count is an important factor in diagnostic medicine. Traditional microscopic methods are labor intensive with variable results and are highly dependent on the individual training. Recent developments in automated peripheral blood differentials using a computerized system have shown many advantages as a viable alternative. The purpose of this paper was to determine the reliability and accuracy of the CellaVision DM 96 system with regards to platelet counts.Materials and Methods:One hundred twenty seven peripheral blood smears were analyzed for platelet count by manual microscopy, an automated hematology analyzer (Beckman Counter LH 780 or Unicel DXH 800 analyzers) and with the CellaVision DM96 system. Results were compared using the correlations and Bland-Altman plots.Results:Platelet counts from the DM96 system showed an R2 of 0.94 when compared to manual platelet estimates and an R2 of 0.92 when compared to the automated hematology analyzer results. Bland-Altman plots did not show any systematic bias.

Detection of Platelet Clumps on Peripheral Blood Smears by CellaVision DM96 System and Microscopic Review

“…overall reliability of each of these functions of the DM96 has been assessed by a number of investigators, [1][2][3][4][5][6][7][8] who found the functions to be acceptable for clinical use. However, to our knowledge, no published studies have assessed the reliability of this system in specifically detecting the presence of PLT clumps on peripheral blood smears.…”

Platelet Counting: Ugly Traps and Good Advice. Proposals from the French-Speaking Cellular Hematology Group (GFHC)

“…This method is useful to avoid artifacts generating over or underestimation of automated platelet counts and has been reported to be more efficient and more accurate than an alternative microscopic method. This is mainly due to the high-resolution scanning of the defined area of the automated blood smear preparation [8].…”

Performance of the CellaVision® DM96 system for detecting red blood cell morphologic abnormalities

“…[ 2 3 4 5 6 7 ] Previous evaluations of these types of systems have shown good correlations with the manual differentiation of normal cells. [ 4 8 9 ]…”