Platelet Concentrates Stored in Synthetic Medium after Filtration

Rebulla, Paolo; Marangoni, Francesca; Sirchia, G.

doi:10.1159/000462173

Cited by 9 publications

(6 citation statements)

References 17 publications

(32 reference statements)

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“…This is in accordance with Bertolini et al [12] who compared filtered and unfiltered platelet pools stored for 15 days in a synthetic medium. Other studies demonstrated that the in vitro and in vivo functional behavior of filtered platelets was satisfactory but no direct comparison was made with unfiltered platelets as was done in our study [13][14][15][16][17][18].…”

Section: Discussionsupporting

confidence: 91%

“…The other method is to sediment at high-Recently leukocyte depletion filters became available for PC. A few studies have shown the effectiveness of these filters [12][13][14][15][16][17][18], but little is known about the influence of fil tration on the quality of the platelets during subsequent stor age of the PC. We therefore evaluated 2 different leukocyte depletion filters for PC which have become available most recently: Pall PL50HF and Sepacell PL10-A.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Filtration on Subsequently Stored Platelet Concentrates

Joustra-Dijkhuis¹,

Boomgaard²,

Pietersz³

et al. 1994

Vox Sanguinis

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The effect of filtration on the quality of platelet concentrates (PC) during storage was investigated. Two leukocyte depletion filters (Pall PL50HF and Sepacell PL-10A) were applied to filter PC made from a pool of 4 huffy coats. For each experiment 3 PC were pooled and divided into 3 identical PC to eliminate differences between the PC. Two PC were filtered, and the third PC served as an unfil- tered control. A total of 12 experiments was performed. Before filtration, volumes of the PC were 263 ± 11.7 ml (mean±SD). Platelet and leukocyte counts per PC were 241±25.9 x 10^9 and7.2±1.8 x 10^6, respectively. After filtration leukocyte counts did not exceed 5 x 10^4 in any of the PC. In the PC filtered with the Pall PL50HF the mean platelet loss was approximately 14% and with the Sepacell PL-10A, 17%. During a 9-day storage period the pH, PO(2), PCO(2), bicarbonate, lactate and glucose concentration and LDH release as well as the morphology, examined by the swirling effect and microscopically, were not significantly different in filtered and unfiltered units. Filtration through the 2 investigated leukocyte depletion filters for PC did not adversely affect in vitro viability of the platelets during storage.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Effect of Filtration on Subsequently Stored Platelet Concentrates

Joustra-Dijkhuis¹,

Boomgaard²,

Pietersz³

et al. 1994

Vox Sanguinis

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“…Glycoprotein (GP) Ib and activation markers were evaluated as previously described [17] by flow cytometry using the followingmonoclonal antibodies: (1) CD42b (clone SZ2, Immunotech, Marseille, France) reactingwith GP Ib; (2) CD62 (clone CLB-thromb/6, Immunotech), reacting with alphagranule activation marker P-Selectin; (3) CD63 (clone CLB-gran 12, Immunotech), reacting with activation marker lysosome-like protein gp 53; (4) 9F9, reacting with plateletbound fibrinogen, a kind gift from Dr. A. Z. Budzynski (Philadelphia, Pa., USA); ( 5 ) D545, reacting withgranulophysin, an activation marker present on platelet dense granule membrane kindly provided by Dr. J.M.…”

Section: Pc Evaluationmentioning

confidence: 99%

Role of Lactate in Platelet Storage Lesion

et al. 1993

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It is known that lactate accumulation may cause a pH fall in platelet concentrates (PC) during storage, and this phenomenon causes platelet morphological lesions and loss of platelet in vivo viability. In this study, we added increasing amounts of lactate to identical PC in order to evaluate the role of hydrogen ion accumulation in determining platelet activation and lesion during storage. Six hours after PC preparation, lactate was added to PC1 and PC2 at 20 and 12 mM final concentrations, respectively, while PC3 served as control. In PC1, pH was lower than 6.3, and platelet function and discoid morphology were lost. PC2 were stored for 7 days at pH values ranging from 6.4 to 6.6, and most results of in vitro measurements reflecting platelet function such as osmotic reversal, ATP release and aggregation in response to different stimuli were not significantly inferior when compared to controls. The addition of lactate had no apparent effect on the rise of platelet activation markers P-Selectin, lysosome-like protein gp 53, platelet-bound fibrinogen and granulophysin, while a reduction of borderline significance was observed in glycoprotein Ib expression after pH reduction to values lower than 6.6. It is concluded that the rise of platelet activation markers during storage reflects platelet lesions different from those determined by lactate per se.

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“…Glycoprotein (GP) lb and activation markers were evaluated as previously described [17] by flow cytometry using the following mono clonal antibodies: (1) CD42b (clone SZ2, Immunotech, Marseille, France) reacting with GP lb; (2) CD62 (clone CLB-thromb/6. Immu notech), reacting with alpha granule activation marker P-Selectin; (3) CD63 (clone CLB-gran 12, Immunotech).…”

Section: Pc Evaluationmentioning

confidence: 99%

Role of Lactate in Platelet Storage Lesion

et al. 1993

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It is known that lactate accumulation may cause a pH fall in platelet concentrates (PC) during storage, and this phenomenon causes platelet morphological lesions and loss of platelet in vivo viability. In this study, we added increasing amounts of lactate to identical PC in order to evaluate the role of hydrogen ion accumulation in determining platelet activation and lesion during storage. Six hours after PC preparation, lactate was added to PCI and PC2 at 20 and 12 mM final concentrations, respectively, while PC3 served as control. In PCI, pH was lower than 6.3, and platelet function and discoid morphology were lost. PC2 were stored for 7 days at pH values ranging from 6.4 to 6.6, and most results of in vitro measurements reflecting platelet function such as osmotic reversal, ATP release and aggregation in response to different stimuli were not significantly inferior when compared to controls. The addition of lactate had no apparent effect on the rise of platelet activation markers P- Selectin, lysosome-like protein gp53, platelet-bound fibrinogen and granu- lophysin, while a reduction of borderline significance was observed in glycoprotein lb expression after pH reduction to values lower than 6.6. It is concluded that the rise of platelet activation markers during storage reflects platelet lesions different from those determined by lactate per se.

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Platelet Concentrates Stored in Synthetic Medium after Filtration

Cited by 9 publications

References 17 publications

Effect of Filtration on Subsequently Stored Platelet Concentrates

Effect of Filtration on Subsequently Stored Platelet Concentrates

Role of Lactate in Platelet Storage Lesion

Role of Lactate in Platelet Storage Lesion

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