Platelet Concentrates: Past, Present and Future

Prakash, Shobha; Thakur, Aditi

doi:10.1007/s12663-011-0182-4

Cited by 100 publications

(93 citation statements)

References 21 publications

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“…PRP contains >30 bioactive proteins including PDGF, TGF-β, VEGF, EGF and bFGF, which have fundamental roles in wound healing (14). These factors are known to regulate processes such as cell migration, attachment, proliferation and differentiation, and promote extracellular matrix (ECM) accumulation by binding to specific cell surface receptors (15).…”

Section: Discussionmentioning

confidence: 99%

Application of platelet-rich plasma accelerates the wound healing process in acute and chronic ulcers through rapid migration and upregulation of cyclin A and CDK4 in HaCaT cells

Kim

Ryu

Lee

et al. 2012

Molecular Medicine Reports

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Abstract. Application of autologous platelet-rich plasma (PRP) has been used for chronic wound healing. The aim of this study was to evaluate the effect of PRP on the wound healing processes of both acute and chronic ulcers and the underlying molecular mechanisms involved. We treated 16 patients affected by various acute and chronic ulcers with PRP. We performed molecular studies of cell proliferation, migration assays, immunoblotting and chloramphenicol acetyltransferase (CAT) assays in PRP-treated HaCaT keratinocyte cells. PRP treatment induced increased rates of cell proliferation and cell migration of HaCaT cells. In addition, the expression of cyclin A and cyclin dependent kinase (CDK) 4 proteins was markedly increased with a low concentration (0.5%) of PRP treatment in HaCaT cells. In 11 patients with chronic ulcers, including stasis ulcers, diabetic ulcers, venous leg ulcers, livedoid vasculitis, claw foot and traumatic ulcers, 9 patients showed 90-100% epithelization after 15.18 days. In 5 patients with acute ulcers, such as dehiscence, open wound and burn wound, 80-100% epithelization was achieved between 4 to 20 days. Topical application of PRP to acute and chronic skin ulcers significantly accelerated the epithelization process, likely through upregulation of the cell cycle regulatory proteins cyclin A and CDK4.

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Section: Discussionmentioning

confidence: 99%

Application of platelet-rich plasma accelerates the wound healing process in acute and chronic ulcers through rapid migration and upregulation of cyclin A and CDK4 in HaCaT cells

Kim

Ryu

Lee

et al. 2012

Molecular Medicine Reports

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“…Platelets are anucleate cytoplasmic fragments containing -granules that are spherical or oval structures with diameters ranging from 200 to 500 nm [5]. They form an intracellular storage pool of proteins vital to wound healing, including platelet-derived growth factor (PDGF), transforming growth factor (TGF ), and insulin-like growth factor (IGF-I).…”

Section: Isrn Biomaterialsmentioning

confidence: 99%

“…They form an intracellular storage pool of proteins vital to wound healing, including platelet-derived growth factor (PDGF), transforming growth factor (TGF ), and insulin-like growth factor (IGF-I). Secretion of the active proteins follows the fusion of the granules with the platelet cell membrane, which subsequently bind to the transmembrane receptors of the target cells [5].…”

Section: Isrn Biomaterialsmentioning

confidence: 99%

“…It consists of the platelets, cytokines, and the fibrin matrix. Platelets and leukocyte cytokines play an important part in the biology of this biomaterial [5]. Degranulation of platelets entails the release of cytokines able to stimulate cell migration and proliferation within the fibrin matrix, launching the first stages of healing [2,3].…”

Section: Biologic Features: Growth Factors Present In Prf Leucocyte mentioning

confidence: 99%

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Platelet-Rich Fibrin as a Biofuel for Tissue Regeneration

Khiste

Tari

2013

ISRN Biomaterials

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Wound healing is a staged process which involves the activity of leukocytes and platelets. For this process to work efficiently, the platelets play a vital role. The growth factors present in platelets are important to guide the regenerating cells to the area of healing. Platelet-rich-fibrin (PRF) is one such material that holds on to these growth factors enmeshed in the fibrin network resulting in their sustained release over a period of time that can accelerate the wound healing process. With this knowledge, research has been carried out for a past few years for the clinical application of PRF. Various platelet concentrates have been studied including the platelet-rich-plasma (PRP). However, the short duration of cytokine release and its poor mechanical properties have resulted in the search of a new material with adequate properties for clinical application and ease of preparation. PRF has found a place in the regenerative field owing to its advantages over PRP. This review focuses on the properties and various applications of PRF in the clinical practice.

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“…Plasma rich platelets-(PRP) has been used medicinally since the 1970 (17) and is obtained from autologous blood because the platelet concentration in PRP is at least 5-fold greater than that in physiological blood. PRP is prepared by centrifuging autologous blood with anticoagulants, such as sodium citrate, which cause the blood to separate into 3 layers in the test tube: Platelet Poor Plasma (PPP), PRP and some red blood cells (17,18) . Blood platelets have no nuclei and are formed through megakaryocyte fragmentation of bone marrow.…”

Section: Introductionmentioning

confidence: 99%

Low Level Laser and Plasma Rich Platelets in Treatment of Hamstrings Strain

Hagag¹

2016

jmscr

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Platelet Concentrates: Past, Present and Future

Cited by 100 publications

References 21 publications

Application of platelet-rich plasma accelerates the wound healing process in acute and chronic ulcers through rapid migration and upregulation of cyclin A and CDK4 in HaCaT cells

Application of platelet-rich plasma accelerates the wound healing process in acute and chronic ulcers through rapid migration and upregulation of cyclin A and CDK4 in HaCaT cells

Platelet-Rich Fibrin as a Biofuel for Tissue Regeneration

Low Level Laser and Plasma Rich Platelets in Treatment of Hamstrings Strain

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