Platelet concentrate injection improves Achilles tendon repair in rats

Aspenberg, Per; Virchenko, Olena

doi:10.1080/00016470410001708190

Cited by 300 publications

(242 citation statements)

References 6 publications

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“…It is presumed that surgical repair combined with the application of biologically active PLG should accelerate healing with an improved outcome. Aspenberg and Virchenko [26] showed, in an in vivo rat model, that PLG applied to traumatized Achilles tendons increased tensile strength and stiffness by about 30% after the first week. The effect persisted for as long as 3 weeks after the injection, suggesting that the use of PLG in tendon repair improved the physiologic healing process.…”

Section: Soft Tissue Healing and Endoscopic Applicationsmentioning

confidence: 99%

The use of autologous platelet–leukocyte gels to enhance the healing process in surgery, a review

et al. 2007

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Background: The therapeutic use of autologously prepared, platelet-leukocyte-enriched gel (PLG) is a relatively new technology for the stimulation and acceleration of soft tissue and bone healing. The effectiveness of this procedure lies in the delivery of a wide range of platelet growth factors mimicking the physiologic wound healing and reparative tissue processes. Despite an increase in PLG applications, the structures and kinetics of this autogenously derived biologic material have not been observed. Methods: A review of the most recent literature was performed to evaluate the use of PLG in various surgical disciplines. Results: The review showed that the application of PLG has been extended to various surgical disciplines including orthopedics, cardiac surgery, plastic and maxillofacial surgery, and recently also endoscopic surgery. Conclusion: This review demonstrates the usefulness of PLG in a wide range of clinical applications for improvement of healing after surgical procedures.Key words: Growth factors -Leukocytes -Platelet gel -Wound healing Soft tissue wound healing and bone growth involve physiologic cascades in which cellular and hormonal factors play pivotal roles [1,2]. Some of these cascade components can be isolated from autologously drawn whole blood. Point-of-care devices can intraoperatively fractionate the autologous blood into platelet-poor plasma, platelet-leukocyte-rich plasma (P-LRP), and red blood cells [3,4]. The P-LRP fraction, a mixture of concentrated platelets and leukocytes, can be activated by (autologous) thrombin to create a viscous solution known as platelet-leukocyte gel (PLG). This platelet coagulum can be exogenously applied to soft wound tissues, bone, or synthetic bone as a spray or as a solid, clotted, gelatinous mass. The rationale for applying platelet gel is based on the delivery of platelet growth factors to tissues and on the fact that platelet a-granules, found inside the platelets, contain a variety of growth factors [5]. Platelet gel growth factors are peptides that promote cell proliferation, differentiation, chemotaxis, and the migration of various cells involved in both wound healing and bone growth [6,7].Recently, numerous P-LRP devices have become available for therapeutic use to stimulate and accelerate soft tissue and bone healing and to control postoperative wound bleeding. The rationale for applying PLG lies in the mimicking and accelerating of physiologic wound healing and reparative tissue processes.This article provides information on the results of electron microscopic imaging used to evaluate the content of PLG. Furthermore, because the use of PLG is a relatively new per-and/or perioperative biotechnological application, new and additional indications for the use of PLG are defined. New PLG applications are reviewed for different applications including cardiac surgery, general surgery, orthopedics and traumatology, cosmetic surgery, maxillofacial surgery, sports medicine, and endoscopic surgical procedures. Defining platelet-leukocyte-rich...

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Section: Soft Tissue Healing and Endoscopic Applicationsmentioning

confidence: 99%

The use of autologous platelet–leukocyte gels to enhance the healing process in surgery, a review

et al. 2007

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“…[32][33][34] In this study, we chose to measure the mediolateral diameter of organized collagen at the rupture site. We are not aware of any other quantitative way of assessing healing histologically.…”

Section: Discussionmentioning

confidence: 99%

Physical activity modulates nerve plasticity and stimulates repair after achilles tendon rupture

Bring

Kreicbergs

Renström

et al. 2006

Journal Orthopaedic Research

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In a rat model of tendon rupture using semiquantitative methodology, healing was assessed according to the diameter of newly organized collagen and the occurrence of the sensory neuropeptides (SP, CGRP) in relation to different levels of physical activity. Normally, innervation of the Achilles tendon is confined to the paratenon. After rupture new nerve fibers grow into the tendon proper, but disappear after healing. In a first experiment to establish peak tissue and nerve regeneration after rupture, tendon tissues from freely moving rats were collected consecutively over 16 weeks. A peak increase in organized collagen and nerve ingrowth was observed between week 2 to 4 post rupture. Therefore, in a second experiment week 4 was chosen to assess the effect of physical activity on tendon healing in three groups of rats, that is, wheel running, plaster treated, and freely moving (controls). In the wheel-running group, the diameter of newly organized collagen was 94% ( p ¼ 0.001) greater than that in the plaster-treated group and 48% ( p ¼ 0.02) greater than that in the controls. Inversely, the neuronal occurrence of CGRP in the tendon proper was 57% ( p ¼ 0.02) lower in the wheel-running group than that in the plaster-treated group and 53% ( p ¼ 0.02) lower than that in the controls, suggesting an earlier neuronal in-growth and disappearance in the more active group. Physical activity speeds up tendon healing, which may prove to be linked to accelerated neuronal plasticity. ß

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“…Therefore, several therapeutic trials regulating endogenous miRNA in vivo have been conducted [14][15][16]. miRNA also plays a crucial role in the pathogenesis of disease or trauma in locomotor organs [17][18][19][20][21]. As we known, vascular supply in tendons is very limited and is significantly reduced after injury or rupture.…”

Section: Introductionmentioning

confidence: 99%

The effect of administration of double stranded MicroRNA-210 on acceleration of Achilles tendon healing in a rat model

Usman

Nakasa

Shoji

et al. 2015

Journal of Orthopaedic Science

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Platelet concentrate injection improves Achilles tendon repair in rats

Cited by 300 publications

References 6 publications

The use of autologous platelet–leukocyte gels to enhance the healing process in surgery, a review

The use of autologous platelet–leukocyte gels to enhance the healing process in surgery, a review

Physical activity modulates nerve plasticity and stimulates repair after achilles tendon rupture

The effect of administration of double stranded MicroRNA-210 on acceleration of Achilles tendon healing in a rat model

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