PLATELET CD62p EXPRESSION AND MICROPARTICLE FORMATION IN MURINE ACQUIRED IMMUNE DEFICIENCY SYNDROME AND CHRONIC ETHANOL CONSUMPTION

Chen, Yinhong; Davis-Gorman, Grace; Watson, Ronald R.; McDonagh, Paul F.

doi:10.1093/alcalc/agg013

Cited by 23 publications

(11 citation statements)

References 39 publications

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“…Evaluation of MPs levels was performed in citrated whole blood, with the advantage to let the MPs in a physiological environment, without preanalytical steps . Ten μL of citrated blood were diluted with 490 μL phosphate‐buffered saline (PBS); Sigma Chemicals, St Louis, Missouri.…”

Section: Methodsmentioning

confidence: 99%

“…Isotype‐matched control antibodies were obtained from Beckman Coulter. PMPs were identified by gating on GPIIb/IIIa (CD41b)‐positive events and distinguished from normal‐sized platelets by forward scatter (FS) size analysis . ErMPs were identified by gating on glycophorin A‐positive events and distinguished from fragmented RBCs by FS size analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Non‐specific binding of the control mouse monoclonal antibodies set the lower fluorescence margin . Ten thousand positive events were analyzed, and MPs were reported as a percentage of total events . Flow cytometric analysis of MPs is presented in Fig.…”

Section: Methodsmentioning

confidence: 99%

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Flow cytometric assessment of circulating platelet and erythrocytes microparticles in young thalassemia major patients: relation to pulmonary hypertension and aortic wall stiffness

Tantawy

Adly

Ismail

et al. 2013

European J of Haematology

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Heart disease is the leading cause of mortality and morbidity in β-thalassemia major (β-TM). Aggregability of abnormal red cells and membrane-derived microparticles (MPs) stemming from activated platelets and erythrocytes are responsible for thrombotic risk. We measured platelet and erythrocyte MPs (PMPs and ErMPs) in 60 young β-TM patients compared with 40 age- and sex-matched healthy controls and assessed their relation to clinicopathological characteristics and aortic elastic properties. Patients were studied stressing on transfusion history, splenectomy, thrombotic events, chelation therapy, hematological and coagulation profiles, flow cytometric measurement of PMPs (CD41b(+) ) and ErMPs (glycophorin A(+) ) as well as echocardiographic assessment of aortic elastic properties. Aortic stiffness index and pulmonary artery pressure were significantly higher, whereas aortic strain and distensibility were lower in TM patients than controls (P < 0.001). Both PMPs and ErMPs were significantly elevated in TM patients compared with controls, particularly patients with risk of pulmonary hypertension, history of thrombosis, splenectomy or serum ferritin >2500 μg/L (P < 0.001). Compliant patients on chelation therapy had lower MPs levels than non-compliant patients (P < 0.001). PMPs and ErMPs were positively correlated to markers of hemolysis, serum ferritin, D-dimer, vWF Ag, and aortic stiffness, whereas negatively correlated to hemoglobin level and aortic distensibility (P < 0.05). We suggest that increased MPs may be implicated in vascular dysfunction, pulmonary hypertension risk, and aortic wall stiffness observed in thalassemia patients. Their quantification could provide utility for early detection of cardiovascular abnormalities and monitoring the biological efficacy of chelation therapy.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Flow cytometric assessment of circulating platelet and erythrocytes microparticles in young thalassemia major patients: relation to pulmonary hypertension and aortic wall stiffness

Tantawy

Adly

Ismail

et al. 2013

European J of Haematology

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“…This reduction can affect the function and survival of PLTs after transfusion. It has been suggested that CD62P acts as a mediator in the clearance of PLTs in the circulation (33)(34)(35). However, some studies did not agree and stated that despite the binding of PLTs to leukocytes via CD62P, this molecule did not have a role in the clearance of PLTs (36).…”

Section: Discussionmentioning

confidence: 99%

The surface markers and survival rate of platelets during storage at 4°C: The influence of sodium octanoate

baghdadi

Yari

Rezaei

et al. 2019

IJPHO

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Background: Storage of platelet concentrates (PCs) at room temperature (20-24°C) limits its storage time to 5 days due to the destructive effects of platelet storage lesion (PSL) and bacterial contamination. Although prolonged storage of platelets (PLTs) at 4°C reduces the likelihood of bacterial contamination and PSL levels, it is accompanied by an increase in the clearance rate and changes in the surface markers of PLTs. The goal of this study was to evaluate the effects of sodium octanoate (SO) as a stabilizer on PLTs during storage at 4°C. Materials and Methods: In this experimental study, PCs were divided into three portions and stored for 5 days at 3 different conditions, including 20-24°C, 4°C temperature, and 4°C in presence of SO. PLTs enumeration was performed using an automated hematology analyzer. To measure the metabolic activity and survival rate of PLTs, the water-soluble tetrazolium salt (WST-1) assay was performed. The activity of lactate dehydrogenase enzyme (LDH) was measured by a biochemical analyzer. Additionally, the levels of PLT glycoprotein Ibα (GPIbα) and CD62P (P-selectin) were measured on PLTs by flow cytometry technique. Results: PLTs count was higher in SO-treated (4°C) PLTs than two other studied samples. Additionally, the viability was higher in the SO-treated PLTs than that in other groups. LDH amount was lower in the SO-treated PLTs than that in other groups (P>0.05). GPIbα expression was significantly higher in SO-treated PLTs than that other groups (P<0.05). On the other hand, the expression of CD62P was lower at 4°C in PLTs in the presence of SO (P>0.05). Conclusions: SO could modulate the effects of cold temperatures on PLTs. Furthermore, we found that the survival of platelets was better maintained in the presence of SO at 4°C.

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“…The modified Tyrode's buffer [134 mM NaCl, 0.34 mM Na 2 HPO 4 , 2.9 mM KCl, 12 mM NaHCO 3 , 20 mM HEPES, 5 mM glucose, and 0.35% (w/v) bovine serum albumin, pH 7.0] was added to bring the sample volume to 100 l. From these diluted samples, aliquots were placed in 12 ϫ 75-mm polypropylene tubes along with the appropriate antibody, collagen (2.5 g/ml), and the modified Tyrode's buffer in a final volume of 200 l. In vitro experiments, N-coumaroyldopamine and N-caffeoyldopamine were dissolved in ethanol and added to diluted blood samples, where the final ethanol volume never exceeded 0.5% (v/v) in both control and test tubes. Samples were analyzed for P-selectin (CD62p) expression on platelets within 1 h of the collection by flow cytometry (Chen et al, 2003;Nieswandt et al, 2005). Data were acquired for 10,000 platelets, and the extent of exposure of CD62p was determined as the measure of platelet activation (FACSCalibur flow cytometer and Cell Quest Pro software; BD Biosciences, San Jose, CA).…”

Section: Methodsmentioning

confidence: 99%

Clovamide-Type Phenylpropenoic Acid Amides,N-Coumaroyldopamine andN-Caffeoyldopamine, Inhibit Platelet-Leukocyte Interactions via Suppressing P-Selectin Expression

Park¹,

Schoene²

2006

J Pharmacol Exp Ther

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N-Coumaroyldopamine and N-caffeoyldopamine are clovamide-type phenylpropenoic acid amides found in Theobroma cacao. In this article, N-coumaroyldopamine and N-caffeoyldopamine were investigated to determine their effects on P-selectin expression and platelet-leukocyte interactions in vitro and in vivo models. At the concentration of 0.05 M, they were able to inhibit P-selectin expression on the platelets by 33 (P Ͻ 0.011) and 30% (P Ͻ 0.012), respectively. The inhibition was partially blocked by ␤ 2 -adrenoceptor antagonists, suggesting that ␤ 2 receptors are probably engaged in the inhibition. NCaffeoyldopamine and N-coumaroyldopamine could also suppress platelet-leukocyte interactions in blood samples by 36 (P Ͻ 0.013) and 32% (P Ͻ 0.011), respectively, at the same concentration (0.05 M). In an animal study, mice administrated orally with N-caffeoyldopamine (50 and 100 g/35 g of body weight) also showed great reduction in the P-selectin expression and platelet-leukocyte interactions by 31 to 45% (P Ͻ 0.011) and 34 to 43% (P Ͻ 0.014), respectively. These data suggest that the clovamide-type phenylpropenoic acid amides are able to suppress platelet-leukocyte interactions via inhibiting P-selectin expression.

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PLATELET CD62p EXPRESSION AND MICROPARTICLE FORMATION IN MURINE ACQUIRED IMMUNE DEFICIENCY SYNDROME AND CHRONIC ETHANOL CONSUMPTION

Abstract: Elevated platelet CD62p and PMPs may represent a pro-thrombotic status that have important pathological consequences.

Cited by 23 publications

References 39 publications

Flow cytometric assessment of circulating platelet and erythrocytes microparticles in young thalassemia major patients: relation to pulmonary hypertension and aortic wall stiffness

Flow cytometric assessment of circulating platelet and erythrocytes microparticles in young thalassemia major patients: relation to pulmonary hypertension and aortic wall stiffness

The surface markers and survival rate of platelets during storage at 4°C: The influence of sodium octanoate

Clovamide-Type Phenylpropenoic Acid Amides,N-Coumaroyldopamine andN-Caffeoyldopamine, Inhibit Platelet-Leukocyte Interactions via Suppressing P-Selectin Expression

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