Platelet binding and protein adsorption to titanium and gold after short time exposure to heparinized plasma and whole blood

Kanagaraja, Sanjiv; Lundström, Ingemar; Nygren, Håkan; Tengvall, Pentti

doi:10.1016/0142-9612(95)00311-8

Cited by 88 publications

(53 citation statements)

References 24 publications

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“…2). Similarly, cell adhesion to the unmodified titanium is mediated by RGD-containing proteins (e.g., fibrinogen, vitronectin [30]) that adsorb non-specifically to titanium and support α V β 3 -mediated adhesion. As demonstrated in the in vitro analyses (Fig.…”

Section: Discussionmentioning

confidence: 99%

The effect of integrin-specific bioactive coatings on tissue healing and implant osseointegration

et al. 2008

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Implant osseointegration, defined as bone apposition and functional fixation, is a requisite for clinical success in orthopaedic and dental applications, many of which are restricted by implant loosening. Modification of implants to present bioactive motifs such as the RGD cell-adhesive sequence from fibronectin (FN) represents a promising approach in regenerative medicine. However, these biomimetic strategies have yielded only marginal enhancements in tissue healing in vivo. In this study, clinical-grade titanium implants were grafted with a non-fouling oligo(ethylene glycol)-substituted polymer coating functionalized with controlled densities of ligands of varying specificity for target integrin receptors. Biomaterials presenting the α 5 β 1 -integrin-specific FN fragment FNIII 7-10 enhanced osteoblastic differentiation in bone marrow stromal cells compared to unmodified titanium and RGD-presenting surfaces. Importantly, FNIII 7-10 -functionalized titanium significantly improved functional implant osseointegration compared to RGD-functionalized and unmodified titanium in vivo. This study demonstrates that bioactive coatings that promote integrin binding specificity regulate marrow-derived progenitor osteoblastic differentiation and enhance healing responses and functional integration of biomedical implants. This work identifies an innovative strategy for the rational design of biomaterials for regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

The effect of integrin-specific bioactive coatings on tissue healing and implant osseointegration

et al. 2008

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mentioning

confidence: 99%

“…and the adsorption of organic species present in body fluids such as proteins. [13][14][15] Furthermore, there is an increasing interest in the study of interfacial behavior of proteins in the human body as a result of problems associated with bacterial growth 16,17 and metal dissolution. 18,19 Considerable insight into the corrosion behavior of CoCrMo biomedical alloys has been obtained using conventional electrochemical techniques such as potentidynamic curves, cyclic voltammetry measurements, potentiostatic tests and Electrochemical Impedance Spectroscopy (EIS).…”

mentioning

confidence: 99%

Passivation of a CoCrMo PVD Alloy with Biomedical Composition under Simulated Physiological Conditions Studied by EQCM and XPS

Valero-Vidal¹,

Muñoz²,

Olsson³

et al. 2012

J. Electrochem. Soc.

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Kinetics of passive film growth on a CoCrMo biomedical alloy have been studied using the Electrochemical Quartz Crystal Microbalance technique (EQCM) in phosphate buffer solution at room temperature and 37 • C. CoCrMo layers were deposited on the quartz crystals by physical vapor deposition (PVD) reaching a dense and compact deposition film with fine-grain structure. EQCM measurements were performed under potentiodynamic and potentiostatic conditions (at applied passive and transpassive potentials). Furthermore, ex-situ X-ray Photoelectron Spectroscopy (XPS) analysis of the each tested sample was performed at the end of the electrochemical test. The use of EQCM allows distinguishing between electrochemical oxidation, passive and transpassive dissolution and passive film growth. In the passive domain the passive film thickness stabilizes within 200 to 400 s after an initial fast growth. The increase in current at the onset of the transpassive domain does not affect the passive dissolution rate. Only at higher potential dissolution rate increases due to the dissolution of Cr(VI), Co(III) and Mo(VI) species. The observed constant mass loss rate at transpassive potentials indicates that the passive film at these potentials is cracked or porous. Increasing temperature accelerates the mass loss through the oxide/electrolyte interface enhancing the passive and transpassive dissolution and increasing the thickness of the oxide film. CoCrMo alloys are one of the most important metallic materials used in orthopedic implants.1-3 Their use is based on their excellent mechanical properties (wear and hardness) and the high resistance to corrosion in physiological media which is related to the spontaneous formation of an oxide film that protects the metal from the surrounding environment. This layer has a high Cr content (mainly Cr 2 O 3 and smaller amount of Cr(OH) 3 ) with a minor contribution of Co and Mo oxides. [4][5][6][7] One of the most important issues in the use of this metallic biomaterial is passive dissolution which has been considered one problem for the long term durability of the implants and for the adverse effects that can cause metal ion release (physiological effects, toxicity, carcinogenicity and metal allergy). [8][9][10][11][12] The properties of the oxide film may change depending on the physico-chemical conditions (i.e. temperature, potential, etc.) and the adsorption of organic species present in body fluids such as proteins. [13][14][15] Furthermore, there is an increasing interest in the study of interfacial behavior of proteins in the human body as a result of problems associated with bacterial growth 16,17 and metal dissolution. 18, 19Considerable insight into the corrosion behavior of CoCrMo biomedical alloys has been obtained using conventional electrochemical techniques such as potentidynamic curves, cyclic voltammetry measurements, potentiostatic tests and Electrochemical Impedance Spectroscopy (EIS). 5,6,[20][21][22][23][24] The Electrochemical Quartz Crystal Microbalance (ECQM) is known to b...

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“…There is an increasing interest in the interfacial behaviour of proteins in the metallic components implanted in the human body as a result of problems associated to bacterial growth (Khan et al, 1996;Kanagaraja et al, 1996) and metal dissolution (Hallab et al, 2001;Virtanen et al, 2008). In the field of biomaterials and medical implants, it is widely accepted that one event that significantly influence the biocompatibility and stability of the biomaterial is the nearly instantaneous adsorption of proteins from biological fluids.…”

Section: Adsorption Of Proteinsmentioning

confidence: 99%

Electrochemical Aspects in Biomedical Alloy Characterization: Electrochemical Impedance Spectrosopy

Vidal¹,

Muñoz²

2011

Biomedical Engineering, Trends in Materials Science

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Platelet binding and protein adsorption to titanium and gold after short time exposure to heparinized plasma and whole blood

Cited by 88 publications

References 24 publications

The effect of integrin-specific bioactive coatings on tissue healing and implant osseointegration

The effect of integrin-specific bioactive coatings on tissue healing and implant osseointegration

Passivation of a CoCrMo PVD Alloy with Biomedical Composition under Simulated Physiological Conditions Studied by EQCM and XPS

Electrochemical Aspects in Biomedical Alloy Characterization: Electrochemical Impedance Spectrosopy

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