Platelet-Associated IgM Levels in Thrombocytopenia

Pawha, Jasjit; Giuliani, Dennis; Morse, Bernard S.

doi:10.1159/000466074

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…Using an immunofluorescent technique, von dem Borne et al (1980) first reported that IgM autoantibodies may occur singly (in 5%) or in conjunction with IgG antibodies (in 24%) in their series of patients with AITP. Pawha et al (1983) reported similar results using a different technique. In contrast, however, Nel et al(l983) found elevated PAIgM levels in a much higher proportion of patients, i.e.…”

Section: Discussionmentioning

confidence: 58%

Platelet associated immunoglobulins (PAIgG and PAIgM) in autoimmune thrombocytopenia

et al. 1985

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An enzyme linked assay system was used to quantitate platelet associated IgM (PAIgM) in addition to platelet associated IgG (PAIgG) in normal subjects and in 145 patients with autoimmune thrombocytopenia (AITP). The mean PAIgM level in normals was 1.17 ng/10(6) platelets with a range of 0.01-2.45 ng (mean +/- 2 SD). The corresponding PAIgG values as follows: mean 6.0 ng, range 2.0-10 ng/10(6) platelets. Elevated PAIgG was seen in 67.6% and abnormally raised PAIgM in 79.3% of patients. Both values were raised together in 57.2% and either elevated PAIgG or PAIgM in 89.7%. All patients with PAIgG values greater than 4 times upper limit of normality were found to have abnormal PAIgM. The relevance of elevated PAIgM, the possible interaction between PAIgG and PAIgM and the implication of our results in patients with autoimmune thrombocytopenia are discussed.

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Section: Discussionmentioning

confidence: 58%

Platelet associated immunoglobulins (PAIgG and PAIgM) in autoimmune thrombocytopenia

et al. 1985

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“…In these cases, the deposition of C3 could result from the presence of anti-platelet antibodies of the IgM class that are known to activate complement more efficiently than most IgG antibodies. Indeed, several investigators have recently observed increased amounts of IgM on the platelets of some patients with ITP (15)(16)(17)(18)(19)(20)(21)(22). Therefore, we sought to determine if complement-fixing IgM anti-platelet antibodies are, in fact, demonstrable in ITP and could account for the presence of C3 on the platelets of some of these patients.…”

Section: Introductionmentioning

confidence: 99%

Platelet antibodies of the IgM class in immune thrombocytopenic purpura.

Cines¹,

Wilson²,

Tomaski³

et al. 1985

J. Clin. Invest.

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The clinical course and response to therapy of patients with immune thrombocytopenic purpura (ITP) are not completely determined by the level of IgG present on the platelet surface. It is possible that antibodies of other immunoglobulin classes also play a role in platelet destruction in some of these patients. Therefore, we studied 175 patients with ITP for the presence of IgM anti-platelet antibodies using radiolabeled polyclonal or monoclonal anti-IgM. We observed that 57% of patients with clinical ITP had increased levels of IgM on their platelets, compared with normal controls and patients with thrombocytopenia who did not have ITP (<10%), (P < 0.01). We obtained similar results using either radiolabeled polyclonal or monoclonal anti-IgM, reagents whose integrity was first characterized using erythrocytes coated with defined amounts of IgM antibody. Among patients with increased platelet-IgM there was a significant correlation both with the presence of increased platelet-C3 as well as the amount of platelet-C3 (P < 0.01, r = 0.53). We demonstrated the presence of warm-reacting IgM antiplatelet antibodies in the plasma of two of these patients who were further studied. The isolated IgM fraction from these two plasmas was able to activate complement and place 3H-C3 on normal platelets. These studies demonstrate the presence of warm-reacting IgM anti-platelet antibodies in some patients with ITP. They suggest that the binding of complement to platelets by IgM antibodies may initiate platelet clearance as well as enhance the effect of IgG antibodies in ITP.

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Platelet-Associated IgM Levels in Thrombocytopenia

Cited by 2 publications

References 8 publications

Platelet associated immunoglobulins (PAIgG and PAIgM) in autoimmune thrombocytopenia

Platelet associated immunoglobulins (PAIgG and PAIgM) in autoimmune thrombocytopenia

Platelet antibodies of the IgM class in immune thrombocytopenic purpura.

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