Platelet Anti-Aggregatory and Adp-Ase Activities of Human Placental Cultures

Abstract

3p Riemann & Pilwat, 1976, Biochimica et Biophysica Acta, 436, 460). We have studied the characteristics in vitro and in vivo of 'ghosts' prepared by this method using a specially constructed apparatus in order to compare these cellular carriers with those prepared by iso-osmotic lysis and to find the optimum preparative technique for cellular carriers for future clinical therapeutic application.Washed erythrocytes from humans or beagle dogs were suspended in iso-osmotic phosphate-buffered potassium chloride (celi/buffer ratio 1 :9, O°C; pH 7·4), placed between plate electrodes 1 cm apart and subjected to electric field pulses of various voltages (2-20 kV) at various capacities (1-4 ,uF).