Platelet aggregation studies in whole human blood

Saniabadi, A.R.; Lowe, G. D. O.; Forbes, C. D.; Prentice, C. R. M.; Barbenel, J.C.

doi:10.1016/0049-3848(83)90271-2

Cited by 50 publications

(19 citation statements)

References 11 publications

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“…Incubation of blood with histones resulted in a drop in platelet but not RBC counts ( Figure 3C). This in vitro thrombocytopenia reflects the loss of individual platelets because of platelet aggregation in blood 17 induced by histones ( Figure 3D). These results indicated that in blood histones bind preferentially to platelets over RBCs in vitro.…”

Section: Extracellular Histones Induce Thrombocytopenia In Micementioning

confidence: 97%

Histones induce rapid and profound thrombocytopenia in mice

Fuchs

Bhandari

Wagner

2011

Blood

371

412

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Section: Extracellular Histones Induce Thrombocytopenia In Micementioning

confidence: 97%

Histones induce rapid and profound thrombocytopenia in mice

Fuchs

Bhandari

Wagner

2011

Blood

371

412

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“…[1][2][3] These methods involve stirring samples of whole blood in the presence of platelet agonists, such as adenosine diphosphate (ADP), and monitoring the fall in the number of single platelets as aggregation occurs. Samples may be fixed so that aggregation can be stopped at a fixed time point.…”

Section: Introductionmentioning

confidence: 99%

Quantitation of platelet aggregation and microaggregate formation in whole blood by flow cytometry

et al. 2004

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Platelet aggregation and microaggregate formation were measured in samples of stirred whole blood by flow cytometry. Blood samples were stirred in a multi-sample agitator with ADP, fixed and labelled with a platelet-specific CD42a-FITC fluorescent antibody. The blood was then diluted and applied directly to a flow cytometer. Platelets were identified using a gating procedure based on their expression of CD42a and then quantified. Aggregation was monitored as a fall in the number of single platelets. Both reversible and irreversible aggregation responses to ADP were determined and these were found to correlate directly with aggregation responses determined using a well-established single platelet counting technique using the Ultra-Flo 100 Whole Blood Platelet Counter. We found from flow cytometry that ADP-induced aggregation was coupled with a transient formation of platelet microaggregates over the initial 60 s following ADP addition. Assessment of single platelet loss by flow cytometry was found to be a reliable way of monitoring aggregation responses and provided new information on rapid microaggregate formation in ADP-stimulated blood.

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“…In their attempt to quantify lymphocyte aggregation in response to extracellular stimuli, Dezorella et al have correlated changes of side and forward scattering to the size of aggregate . Platelets as functional cellular fragments in blood are undergoing aggregation upon activation, and scattering shifts in platelets population was useful to analyze FCM results and assess the percentage of activated platelets . Harding et al have also employed FCM techniques to investigate effects of collection and handling variables on platelets activation by quantifying circulating platelets‐monocyte aggregates .…”

mentioning

confidence: 99%

“…. Platelets as functional cellular fragments in blood are undergoing aggregation upon activation, and scattering shifts in platelets population was useful to analyze FCM results and assess the percentage of activated platelets (15)(16)(17). Harding et al have also employed FCM techniques to investigate effects of collection and handling variables on platelets activation by quantifying circulating platelets-monocyte aggregates (18).…”

mentioning

confidence: 99%

Two‐Color Analysis of Leukocytes Labeled by Modified RBCs and Their Fragments

2018

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Red blood cells (RBCs) are attractive tools for surface modification to adhere specifically to molecules, cellular fragments (e.g., microvesicles), or whole cells for potential use in bioanalytical assays or as a delivery vehicle in targeted therapy. Within this study, we have loaded RBCs with fluorochrome‐conjugated antibodies (Ab) against CD45 and CD22 leukocyte markers and evaluated the conjugation process by microscopy. We have assessed the potential application of RBCs fragments generated from conjugated RBCs for targeting Cyto‐Trol control cells by flow cytometric (FCM) approaches. Based on their scattering and fluorescence characteristics (FITC and PE expression), modified RBCs and their fragments, Cyto‐Trol cells, and clusters of both were distinguished by two color FCM analysis. Fragments with anti‐human Kallestad Ab as a nonspecific FITC conjugate had less than 20% binding to Cyto‐Trol controls compared to CD45‐FITC Ab conjugate with nearly 100% binding capacity. Cyto‐Trol‐microvesicle‐clusters were more than 45% positive for either FITC or PE. Anti‐CD22‐PE modified RBCs fragments were also useful in staining and showing about 19.5% positively stained events in the Cyto‐Trol region. The proof‐of‐concept shows, that specific antibody can be attached to RBCs, and generated fragments can be useful to stain target cells for FCM analysis. © 2018 International Society for Advancement of Cytometry

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Platelet aggregation studies in whole human blood

Cited by 50 publications

References 11 publications

Histones induce rapid and profound thrombocytopenia in mice

Histones induce rapid and profound thrombocytopenia in mice

Quantitation of platelet aggregation and microaggregate formation in whole blood by flow cytometry

Two‐Color Analysis of Leukocytes Labeled by Modified RBCs and Their Fragments

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