Platelet adsorption and hemolytic properties of liquid crystal/composite polymers

Shih, Mei‐Fen; Shau, Min‐Da; Chang, Meng-Ying; Chiou, Se-Kai; Chang, Jiunn-Kae; Cherng, Jong Yuh

doi:10.1016/j.ijpharm.2006.07.043

Cited by 143 publications

(85 citation statements)

References 21 publications

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“…The blood clotting test was adapted from Shih et al [18]. Dressings were placed into polypropylene tubes, and prewarmed to 37 C. Citrated whole blood (0.2 mL) was then dispensed onto the dressings, and 20 mL of 0.2 M CaCl 2 solution added to start coagulation.…”

Section: Whole Blood Clottingmentioning

confidence: 99%

Development of a chitosan-based wound dressing with improved hemostatic and antimicrobial properties

et al. 2008

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Section: Whole Blood Clottingmentioning

confidence: 99%

Development of a chitosan-based wound dressing with improved hemostatic and antimicrobial properties

et al. 2008

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“…The blood clotting assay was performed based on the procedure described in literature [32,33]. Blood (5 ml) was taken from healthy volunteers and 10% acid citrate dextrose (ACD) was added as anticoagulant.…”

Section: Whole Blood Clotting Assaymentioning

confidence: 99%

Triethyl orthoformate mediated a novel crosslinking method for the preparation of hydrogels for tissue engineering applications: characterization and in vitro cytocompatibility analysis

Yar

Shahzad

Siddiqi

et al. 2015

Materials Science and Engineering: C

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“…21 Clear separation of the upper plasma layer from the red blood cells was observed. The top one milliliter of the plasma layer was retrieved and designated as PRP.…”

Section: Blood Collectionmentioning

confidence: 94%

Beyond Cell Capture: Antibody Conjugation Improves Hemocompatibility for Vascular Tissue Engineering Applications

Chong

Teoh

Teo

et al. 2010

Tissue Engineering Part A

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Antibody-conjugated surfaces are being studied for cardiovascular implant applications to capture endothelial progenitor cells and promote endothelialization. However, despite the large amount of literature on endothelial progenitor cell capture efficiency, little effort has been made to understand acute blood responses to the modified surfaces. We hypothesize that CD34 antibody conjugation passivates surfaces against procoagulatory events, and thus improves hemocompatibility. To test this hypothesis, we subjected the modified films to hemocompatibility tests to evaluate contact activation, platelet adhesion and activation, as well as whole blood clotting response to the films. Here, we demonstrate the alteration of blood responses due to polyacrylic acid (PAAc) engraftment and subsequent antibody conjugation on biaxially stretched polycaprolactone (PCL) films. Compared to PCL, PAAc-engrafted PCL (PCL-PAAc) and CD34-antibody-conjugated films (PCL-PAAC-CD34) resulted in a four- to ninefold (p < 0.001) reduced platelet activation. PCL-PAAc, however, resulted in an increased contact activation on thromboelastography, and a poorer blood compatibility index assay (43.4% +/- 2.3% vs. 60.9% +/- 2.5%, p < 0.05). PCL-PAAC-CD34, on the other hand, resulted in delayed clot formation (r = 19.3 +/- 1.5, k = 6.8 +/- 0.6 min) and reduced platelet adhesion and activation, and yielded the highest blood compatibility index score, indicating least thrombogenicity (69.3% +/- 3.2%). Our results suggest that CD34 antibody conjugation significantly improved the hemocompatibility of PAAc-conjugated PCL.

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Platelet adsorption and hemolytic properties of liquid crystal/composite polymers

Cited by 143 publications

References 21 publications

Development of a chitosan-based wound dressing with improved hemostatic and antimicrobial properties

Development of a chitosan-based wound dressing with improved hemostatic and antimicrobial properties

Triethyl orthoformate mediated a novel crosslinking method for the preparation of hydrogels for tissue engineering applications: characterization and in vitro cytocompatibility analysis

Beyond Cell Capture: Antibody Conjugation Improves Hemocompatibility for Vascular Tissue Engineering Applications

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