Platelet adhesion to dengue-2 virus-infected endothelial cells.

Krishnamurti, Chitra; Peat, Raquel A.; Cutting, Mary A.; Rothwell, Stephen W.

doi:10.4269/ajtmh.2002.66.435

Cited by 78 publications

(89 citation statements)

References 24 publications

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“…20 Studies report that the platelet activation (with elevated surface P-selectin) and apoptosis (with increased caspases and phosphatidylserine (PS) expression) are associated in the early days of dengue infection. 21,22 Reports also show that the activation of complement factor C3 followed by binding of C5b-9 complex to platelet surface is significantly linked with platelet destruction and thrombocytopenia in these patients. [23][24][25] 11 of 61 patients studied by Mitrakul et al, had destruction as a main cause for thrombocytopenia, as revealed by platelet kinetic study.…”

Section: Discussionmentioning

confidence: 97%

Platelet indices evaluation in patients with dengue fever

Mukker

Kiran

2018

Int J Res Med Sci

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Background: Platelet indices (PIs)-Platecrit, mean platelet volume (MPV) and platelet distribution width (PDW)-are a group of platelet parameters obtained as a part of complete blood count using automated hematology analyzers. Evidence suggests that PIs may have diagnostic and prognostic value in febrile thrombocytopenia. This study aims to understand the profile of PIs in dengue fever. Aims and objectives was to study the platelet indices in patients with dengue fever.Methods: The present study is a retrospective observational study conducted in a tertiary hospital in Kerala. Platelet count, mean platelet volume (MPV), platecrit and Platelet distribution width (PDW) along with routine blood parameters hemoglobin(Hb), hematocrit, WBC, Platelet count, serum bilirubin, liver enzymes (AST, ALT) of 123 patients were collected. These 123 patients were grouped into three according to the platelet count (<20000,20000-100000, >100000). All the test results are available in hospital database. This was accessed using inpatient numbers obtained from medical records department of our institution. All analysis was done using free to use software R and values were rounded off to the nearest decimal point. Non normally distributed parameters were expressed as median (IQR). Parameters which did not follow normal distribution were analyzed with Kruskall Wallis test and the values are expressed as mean (SD) and a p value <0.05 was considered statistically significant.Results: Platelet indices PDW (57±13.8 vs. 55.4±6.9, p value 0.001) and MPV (9.2±0.09 vs. 13.8±1.3fL, p value <0.001) values were significantly altered in dengue fever with platelet counts below 20,000 compared to platelet count more than one lakh group. Similarly, the Platelet index (MPVxPDW\PLCxPCT), MPV\PLC, MPV\Platecrit, PDW\PLC and PDW\Platecrit ratio showed statistically significant difference between the different platelet groups.Conclusions: Platelet indices are useful parameters in dengue infection. Other than platelet count, PDW, MPV, platecrit are useful to monitor dengue fever.

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Section: Discussionmentioning

confidence: 97%

Platelet indices evaluation in patients with dengue fever

Mukker

Kiran

2018

Int J Res Med Sci

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“…It is possible that the decrease in the TEER before 48 h after addition of PBMCs is due to activation of PBMCs by adherence to the DV-2-infected endothelial cell monolayer. After infection with dengue virus, endothelial cells express adhesion molecules (Krishnamurti et al, 2002). PBMCs which attached to infected endothelial cells are activated, and activated PBMCs may produce cytokines that act locally and increase plasma leakage.…”

Section: Discussionmentioning

confidence: 99%

“…Endothelial cells can be infected with dengue virus in vitro (Bunyaratvej et al, 1997;Huang et al, 2003;Killen & O'Sullivan, 1993). Dengue virus infection induces cytokine production (Avirutnan et al, 1998;Bosch et al, 2002) and alters the expression of adhesion molecules on endothelial cells (Krishnamurti et al, 2002). Although endothelial cells are not massively infected, there is evidence of dengue virus infection of endothelial cells in vivo (Gubler & Zaki, 1998;Hall et al, 1991;Jessie et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Peripheral blood mononuclear cells increase the permeability of dengue virus-infected endothelial cells in association with downregulation of vascular endothelial cadherin

Dewi

Takasaki

Kurane

2008

Journal of General Virology

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Plasma leakage is one of the characteristic features of dengue haemorrhagic fever. The interaction among peripheral blood mononuclear cells (PBMCs), dengue virus and endothelial cells was analysed in vitro. Human umbilical vein endothelial cells (HUVECs) were infected with dengue-2 virus (DV-2) at an m.o.i. of 0.5 p.f.u. per cell. PBMCs were added to DV-2-infected HUVECs, and transendothelial electrical resistance (TEER) and transalbumin permeability were assessed. Dengue virus infection at an m.o.i. of 0.5 p.f.u. per cell alone did not decrease the TEER, but addition of PBMCs decreased the TEER, increased the albumin permeability and induced morphological changes of HUVECs. The extent of the decrease was more profound with adherent PBMCs than with non-adherent PBMCs. The expression of vascular endothelial cadherin (VE-cadherin) was examined using real-time RT-PCR and immunofluorescence. Addition of PBMCs to DV-2-infected HUVECs decreased the levels of mRNA transcripts and cell-surface expression of VE-cadherin. The results indicate that PBMCs increased the permeability of DV-2-infected HUVECs and that the increased permeability was concomitant with morphological change and the decrease in VE-cadherin expression. The results suggest that functional impairment of the DV-2-infected HUVEC monolayer was caused by interaction with PBMCs.

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“…More importantly, it is possible that the formation of PAIgM could be linked to the increased vascular permeability, which is characteristic of DHF. A recent in vitro study has reported on the adhesion of platelets to dengue virus-infected endothelial cells [29]. Activated platelets associated with dengue virus and antidengue virus IgM as well as dengue virus-infected endothelial cells may be involved in the mechanism of the increased vascular permeability associated with DHF [30,31].…”

Section: Discussionmentioning

confidence: 99%

Association of increased platelet-associated immunoglobulins with thrombocytopenia and the severity of disease in secondary dengue virus infections

Saito¹,

Oishi²,

Inoue

et al. 2004

Clinical and Experimental Immunology

106

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SUMMARY Severe thrombocytopenia and increased vascular permeability are two major characteristics of dengue haemorrhagic fever (DHF). To develop a better understanding of the roles of platelet-associated IgG (PAIgG) and IgM (PAIgM) in inducing thrombocytopenia and its severity of disease in patients with secondary dengue virus infection, the relationship between the PAIgG or PAIgM levels and disease severity as well as thrombocytopenia was examined in 78 patients with acute phase secondary infection in a prospective hospital-based study. The decrease in platelet count during the acute phase recovered significantly during the convalescent phase. In contrast, the increased levels of PAIgG or PAIgM that occurred during the acute phase of these patients decreased significantly during the convalescent phase. An inverse correlation between platelet count and PAIgG or PAIgM levels was found in these patients. Anti-dengue virus IgG and IgM activity was found in platelet eluates from 10 patients in an acute phase of secondary infection. Increased levels of PAIgG or PAIgM were significantly higher in DHF than those in dengue fever (DF). An increased level of PAIgM was associated independently with the development of DHF, representing a possible predictor of DHF with a high specificity. Our present data suggest that platelet-associated immunoglobulins involving antidengue virus activity play a pivotal role in the induction of thrombocytopenia and the severity of the disease in secondary dengue virus infections.

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Platelet adhesion to dengue-2 virus-infected endothelial cells.

Cited by 78 publications

References 24 publications

Platelet indices evaluation in patients with dengue fever

Platelet indices evaluation in patients with dengue fever

Peripheral blood mononuclear cells increase the permeability of dengue virus-infected endothelial cells in association with downregulation of vascular endothelial cadherin

Association of increased platelet-associated immunoglobulins with thrombocytopenia and the severity of disease in secondary dengue virus infections

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