Platelet-activating factor and oxidized phosphatidylcholines do not suppress endotoxin-induced pro-inflammatory signaling among human myeloid and endothelial cells

Jacob, Shancy Petsel; Lakshmikanth, Chikkamenahalli Lakshminarayana; McIntyre, Thomas M.; Marathe, Gopal K.

doi:10.3934/allergy.2017.3.108

Cited by 2 publications

(3 citation statements)

References 56 publications

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“…Lipidomic studies made on pathological tissues could demonstrate that among OxPLs, those containing choline residues were more abundant [ 23 , 27 ]. Two main species, POVPC (1-palmitoyl-2-(5-oxovaleroyl)- sn -glycero-3-phosphocholine) and PGPC (1-palmitoyl-2-glutaroyl- sn -glycero-3-phosphocholine) ( Figure 1 B), are able to activate endothelial cells to bind monocytes [ 28 , 29 ]. Moreover, the bioactivity of these compounds has been demonstrated on vascular cells, leukocytes, and platelets [ 30 , 31 , 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of Truncated Oxidized Phosphatidylcholines on Phospholipase A2 Activity in Mono- and Polyunsaturated Biomimetic Vesicles

Yordanova

Hazarosova

Vitkova

et al. 2023

IJMS

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The interplay between inflammatory and redox processes is a ubiquitous and critical phenomenon in cell biology that involves numerous biological factors. Among them, secretory phospholipases A2 (sPLA2) that catalyze the hydrolysis of the sn-2 ester bond of phospholipids are key players. They can interact or be modulated by the presence of truncated oxidized phosphatidylcholines (OxPCs) produced under oxidative stress from phosphatidylcholine (PC) species. The present study examined this important, but rarely considered, sPLA2 modulation induced by the changes in biophysical properties of PC vesicles comprising various OxPC ratios in mono- or poly-unsaturated PCs. Being the most physiologically active OxPCs, 1-palmitoyl-2-(5′-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC) and 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC) have been selected for our study. Using fluorescence spectroscopy methods, we compared the effect of OxPCs on the lipid order as well as sPLA2 activity in large unilamellar vesicles (LUVs) made of the heteroacid PC, either monounsaturated [1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)], or polyunsaturated [1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (PDPC)] at a physiological temperature. The effect of OxPCs on vesicle size was also assessed in both the mono- and polyunsaturated PC matrices. Results: OxPCs decrease the membrane lipid order of POPC and PDPC mixtures with PGPC inducing a much larger decrease in comparison with POVPC, indicative that the difference takes place at the glycerol level. Compared with POPC, PDPC was able to inhibit sPLA2 activity showing a protective effect of PDPC against enzyme hydrolysis. Furthermore, sPLA2 activity on its PC substrates was modulated by the OxPC membrane content. POVPC down-regulated sPLA2 activity, suggesting anti-inflammatory properties of this truncated oxidized lipid. Interestingly, PGPC had a dual and opposite effect, either inhibitory or enhancing on sPLA2 activity, depending on the protocol of lipid mixing. This difference may result from the chemical properties of the shortened sn-2-acyl chain residues (aldehyde group for POVPC, and carboxyl for PGPC), being, respectively, zwitterionic or anionic under hydration at physiological conditions.

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Section: Introductionmentioning

confidence: 99%

Impact of Truncated Oxidized Phosphatidylcholines on Phospholipase A2 Activity in Mono- and Polyunsaturated Biomimetic Vesicles

Yordanova

Hazarosova

Vitkova

et al. 2023

IJMS

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“…E. coli is a gram-negative bacterium which contains the endotoxin lipopolysaccharide (LPS). LPS binds to TLR-4 on microglia and elicits a strong immune response; including, initiating the translocation of NFkB to the nucleus for the transcription of pro-inflammatory genes such as COX-2 and IL-8 (Petsel, et al, 2017). It further induces the production of various immunoregulators, proinflammatory cytokines, free radicals and a deficit in fractalkine receptor CX3CR1, which collectively damage DA neurons in SNc (Wang, Liu & Zhou, 2015).…”

Section: Gut-brain Axismentioning

confidence: 99%

“…It further induces the production of various immunoregulators, proinflammatory cytokines, free radicals and a deficit in fractalkine receptor CX3CR1, which collectively damage DA neurons in SNc (Wang, Liu & Zhou, 2015). Platelet-activating factor (PAF) and related phospholipid products (OxPls) are also made in response to LPS, which further increases inflammation (Petsel, et al, 2017).…”

Section: Gut-brain Axismentioning

confidence: 99%

The Impact of Bacteriophage on the Aging Brain and Inflammatory Response: Relevance to Parkinson’s Disease

Beauchamp¹

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Parkinson's disease (PD) is associated with age and inflammation. New studies have found a link between gut dysbiosis and the prevalence of PD. Phage 936, which is found in dairy products, has been associated with disruptions in the gut microbiome and leaky gut causalities. The present thesis sought to assess the impact of phage 936 in young or old mice and whether the virus augments or diminishes the impact of an inflammatory (LPS) stimulus. To this end, an initial study was conducted to first determine if bacteriophage alone could actually produce some degree of measurable changes (e.g. neutrophil mobilization or change in cytokine or other circulating

show abstract

Platelet-activating factor and oxidized phosphatidylcholines do not suppress endotoxin-induced pro-inflammatory signaling among human myeloid and endothelial cells

Cited by 2 publications

References 56 publications

Impact of Truncated Oxidized Phosphatidylcholines on Phospholipase A2 Activity in Mono- and Polyunsaturated Biomimetic Vesicles

Impact of Truncated Oxidized Phosphatidylcholines on Phospholipase A2 Activity in Mono- and Polyunsaturated Biomimetic Vesicles

The Impact of Bacteriophage on the Aging Brain and Inflammatory Response: Relevance to Parkinson’s Disease

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