Plate in the zone of oocyte and germinal epithelium contact in Scyphomedusa Aurelia aurita binds antibodies to ZP-domain protein mesoglein

Adonin, Leonid S.; Podgornaya, O. I.; Matveev, I. V.; Shaposhnikova, T. G.

doi:10.1134/s1990519x09030109

Cited by 6 publications

(14 citation statements)

References 15 publications

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“…2, Fig. 6) [19]. The internal position of the granules at early stages of oogenesis and the position of the plate itself led us to the suggestion that trophocytes could be involved in the synthesis of their material only to the extend of providing liquid nutritions.…”

Section: Discussionmentioning

confidence: 99%

Aurelia aurita (Cnidaria) Oocytes' Contact Plate Structure and Development

2012

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One of the A. aurita medusa main mesoglea polypeptides, mesoglein, has been described previously. Mesoglein belongs to ZP-domain protein family and therefore we focused on A.aurita oogenesis. Antibodies against mesoglein (AB RA47) stain the plate in the place where germinal epithelium contacts oocyte on the paraffin sections. According to its position, we named the structure found the “contact plate”. Our main instrument was AB against mesoglein. ZP-domain occupies about half of the whole amino acid sequence of the mesoglein. Immunoblot after SDS-PAGE and AU-PAGE reveals two charged and high Mr bands among the female gonad germinal epithelium polypeptides. One of the gonads' polypeptides Mr corresponds to that of mesogleal cells, the other ones' Mr is higher. The morphological description of contact plate formation is the subject of the current work. Two types of AB RA47 positive granules were observed during progressive oogenesis stages. Granules form the contact plate in mature oocyte. Contact plate of A.aurita oocyte marks its animal pole and resembles Zona Pellucida by the following features: (1) it attracts spermatozoids; (2) the material of the contact plate is synthesized by oocyte and stored in granules; (3) these granules and the contact plate itself contain ZP domain protein(s); (4) contact plate is an extracellular structure made up of fiber bundles similar to those of conventional Zona Pellucida.

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Section: Discussionmentioning

confidence: 99%

Aurelia aurita (Cnidaria) Oocytes' Contact Plate Structure and Development

2012

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“…But a subsequent autoradiographic study of the trophic function of the "nurse cells" did not confirm their participation in processes of transferring nutri ents and yolk to the oocyte (Avian et al, 1987). Results of a histochemical study using differential staining indicate that the chemical composition of the plate and granules is different from that of the surrounding yolk (Adonin et al, 2009). The absence of the trophic function in the nurse cells also gives evidence in favor of the synthesis of the plate's material by the oocyte itself.…”

Section: Discussionmentioning

confidence: 92%

“…Their relative molecular weights are 180 and 210 kDa (Adonin et al, 2009), i.e., more than that of mesoglein (molecular weight 47 kDa). It is likely that proteins from these zones are the ones present in the specific granules of the oocyte and in the contact plate.…”

Section: Discussionmentioning

confidence: 99%

“…It is probably the ZP domain of mesoglein that polyclonal serum antibodies RA47 recognize. In paraffin sections of gonad tissues of mature A. aurita females, the anti bodies reveal the presence of mesoglein material in the contact area between the oocyte and the germinal epi thelium (Adonin et al, 2009). The morphology and dynamics of the contact plate formation require a description, and the purpose of this study is to provide such a description.…”

Section: Introductionmentioning

confidence: 99%

“…B-schematic diagram of the gonad (fromEckelbarger and Larson, 1988, modified). 1-5 are stages of oocyte development according toEckelbarger and Larson (1988); I-VII are stages of oocyte development according toAdonin et al (2009).…”

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confidence: 99%

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