Plastidial and Mitochondrial Malonyl CoA-ACP Malonyltransferase is Essential for Cell Division and Its Overexpression Increases Storage Oil Content

Integrated Analysis of Comparative Lipidomics and Proteomics Reveals the Dynamic Changes of Lipid Molecular Species in High-Oleic Acid Peanut Seed

“…Consequently, nine DAPs (Figure ) were identified to possess a similar trend with the DELs that were involved in high-OA peanut TAGs assembly. Indeed, MCMT , PP , KAR , and two GPAT were only correlated with the expressions of LysoPC 15:0. PC 3 enzyme was solely associated with PC, di-acyl-glycerols (DAGs), and TAGs family species.…”

Melatonin Represses Oil and Anthocyanin Accumulation in Seeds

“…Log 2 Ratios Functions References WRI1 (AT3G54320) 0.69 Promoting oil accumulation Focks and Benning, 1998;Cernac and Benning, 2004;Masaki et al, 2005;Baud et al, 2007;Maeo et al, 2009;To et al, 2012 BCCP1 (AT2G38040) 0.63 Promoting oil accumulation Ohlrogge et al, 1995;Sasaki et al, 1995;Mu et al, 2008;Li et al, 2011 CAC3 (AT2G38040) 0.66 Promoting oil accumulation Ohlrogge et al, 1995;Sasaki et al, 1995;Mu et al, 2008;Li et al, 2011 MCAMT (AT2G30200) 0.60 Promoting oil accumulation Jung et al, 2019;Mu et al, 2008 PLIP1 (AT3G61680) 0.65 Promoting oil accumulation Wang et al, 2017a GPAT2 (AT1G02390) 0.96 Exhibiting sn-1 and sn-2 acyltransferase activities and utilizing dicarboxylic acyl-CoA as the substrate for the biosynthesis of the extracellular lipids Beisson et al, 2007;Yang et al, 2012;Jayawardhane et al, 2018 LTP3 (AT5G59320) 2.42 Promoting soluble sugar accumulation and enhancing the in vitro transfer of phospholipids between membranes and binding acyl chains with no obvious effect on oil accumulation in the single mutant Kader, 1996;Arondel et al, 2000;Pagnussat et al, 2015;Wong et al, 2017 LTP4 (AT5G59310) 1.19 Enhancing the in vitro transfer of phospholipids between membranes and binding acyl chains Kader, 1996;Arondel et al, 2000;Wong et al, 2017 LTP5 (AT3G51600) 1.06 Enhancing the in vitro transfer of phospholipids between membranes and binding acyl chains anthocyanins, and proanthocyanidins (PAs; Lepiniec et al, 2006). Considering that biosynthesized flavonols are converted into both anthocyanins and PAs in the flavonoid biosynthetic pathway (Lepiniec et al, 2006), we speculated that anthocyanins and PAs compete against each other for flavonols during flavonoid biosynthesis.…”

“…The complete loss of BCCP1 function results in embryo lethality, and reduced BCCP1 activity markedly decreases FA accumulation in Arabidopsis seeds (Li et al, 2011). MCAMT, localized in both chloroplasts and mitochondria, converts malonyl-CoA and ACYL CAR-RIER PROTEIN (ACP) into malonyl-ACP and CoA, and significantly promotes oil accumulation in Arabidopsis seeds (Jung et al, 2019). PLIP1, as a chloroplast thylakoid-associated protein, functions in the export of FAs from the chloroplast and the incorporation of FAs derived from the thylakoid membrane lipid pool into TAG, and positively regulates FA accumulation in Arabidopsis seeds (Wang et al, 2017a).…”

Differential Activation of Partially Redundant Δ9 Stearoyl-ACP Desaturase Genes Is Critical for Omega-9 Monounsaturated Fatty Acid Biosynthesis During Seed Development in Arabidopsis

“…Examples of EMB genes encoding plastidic enzymes of late glycolysis and FA synthesis include EMB3003, coding for a dihydrolipoamide acetyltransferase, E2 component of the pyruvate dehydrogenase complex (Lin et al, 2003), PYRUVATE DEHYDROGENASE E1 ALPHA (PDH-E1 ALPHA), E1a component of the same complex (Savage et al, 2013), HCS1, encoding an holocarboxylase synthetase catalyzing the addition of biotin to acetyl-CoA carboxylase (Meinke, 2019), EMB3147, encoding a malonyl-CoA: ACP malonyltransferase (Jung et al, 2019), BETA-KETOACYL-ACP SYNTHETASE2 (KASII/FAB1) (Pidkowich et al, 2007), encoding a component of the FA synthase complex, and LIP1, encoding a lipoyl synthase (Ewald et al, 2013). KASI deficiency was also reported to result in disrupted embryo development before the globular stage, and it is tempting to speculate that the incomplete penetrance of this phenotype in the T-DNA mutant line characterized is the consequence of the location of the T-DNA insertion in the 5'-UTR of the gene (Wu et al, 2010).…”