Plastid differentiation and chlorophyll synthesis in cotyledons of black pine seedlings grown in the dark

Nikolić, D.; Bogdanovic, M.

doi:10.1007/bf01279816

Cited by 23 publications

(9 citation statements)

References 4 publications

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“…In young tissues, secondary metabolism begins later, e.g., tannins only appear after germination of the seeds. Nicolić and Bodganović (1972) have suggested that the differentiation rate of plastids varies in embryos, and that embryos contain both protochlorophyll and chlorophyll. The variations in the plastid differentation rate, PPO concentration in the plastids, and the onset of tannin synthesis might explain the differences in browning at the maturation stage in cultures from immature seeds.…”

Section: Discussionmentioning

confidence: 99%

Tissue browning of in vitro cultures of Scots pine: Role of peroxidase and polyphenol oxidase

Laukkanen

Häggman²,

Kontunen-Soppela

et al. 1999

Physiologia Plantarum

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Callus cultures from shoot tips of mature Scots pine (Pinus sylvestris L.) were characterized by rapid browning and an inability to regenerate. The peroxidase (POD) and polyphenol oxidase (PPO) activities and relationship to browning in such cultures were compared with embryogenic and non‐embryogenic cultures of Scots pine, started from immature embryos of three different pine clones. The browning in callus cultures derived from pine buds was visible approximately after 2 weeks of culture, and continued thereafter until the callus was dark brown and poorly growing. The non‐embryogenic cultures induced from immature embryos showed either light yellow coloring or browning, whereas the embryogenic cultures showed browning. POD activity increased during the first 4 weeks in callus tissue initiated from pine buds, and was significantly higher than in pine buds or cultures derived from immature embryos. The ability of cultures initiated from pine buds to oxidize catechol was notably high compared with cultures initiated from immature embryos, regardless of the time of measurement. Addition of catalase revealed that both POD and PPO were able to use catechol as substrate. An antibody raised against broad bean (Vicia faba) chloroplast PPO was used to recognize PPO. One polypeptide with a molecular mass of 50 kDa was detected in all pine samples on SDS‐PAGE and non‐denaturing PAGE. Another polypeptide with a molecular mass of 70 kDa was shown exclusively in the light‐yellow non‐embryogenic cultures. The results suggest that especially the high POD activities in callus tissues started from mature trees cause rapid and early browning and possibly subsequent cell death.

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Section: Discussionmentioning

confidence: 99%

Tissue browning of in vitro cultures of Scots pine: Role of peroxidase and polyphenol oxidase

Laukkanen

Häggman²,

Kontunen-Soppela

et al. 1999

Physiologia Plantarum

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“…It has been observed with electron microscopy that the plastids of dark-grown conifer seedlings contained lamellae, grana and prolamellar bodies {e.g. Oku et al 1975, Michel-Wolwertz et al 1974, Nikolic et al 1972, Anikushin 1971. In some papers (Michel-Wolwertz et al 1974, Anikushin 1971, the plastids of dark-grown seedlings were compared with those of seedlings grown under continuous illumination.…”

Section: Discussionmentioning

confidence: 99%

Photoactivation of Oxygen‐evolving System in Dark‐grown Spruce Seedlings

Oku

Tomita

1976

Physiologia Plantarum

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Plastids prepared from dark‐grown spruce seedlings showed a negligible activity of photosystem II, and no fluorescence variation was observed during actinic illumination. The photosystem II reaction centre, however, was present in primary thylakoids. Exposure of such seedlings to continuous light induced the development of photosystem II activity via three stages (rapid, lagged and gradual), and the variable fluorescence appeared. The rapid development of photosystem II may be attributed to the activation of the oxygen‐evolving system, possibly the manganese‐catalyzing site, and the lagged and gradual developments may be closely related to the formation of thylakoid membranes and their assembly to grana.

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“…Chl synthesis commences a few days after soaking and chloroplasts with well developed granal and stromal lamellae are formed in the complete absence of light (Nikloli6 and Bogdanovi6 1972). These frequently contain small prolamellar bodies and phototransformable Pchlide (Wolwertz and Brouers 1980).…”

Section: Introductionmentioning

confidence: 99%

Immunodetection and photostability of NADPH-protochlorophyllide oxidoreductase in Pinus pinea L.

Packer

Adamson

1990

Photosynth Res

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Antibody against the light-dependent NADPH-protochlorophyllide oxidoreductase of oat was used to detect a protein of the same molecular weight in cotyledons of 40-day-old dark-grown seedlings of Pinus pinea L. Exposure of the seedlings to light resulted in a rapid decrease in protochlorophyllide content without the concomitant decrease in 38 kDa protein which is observed on transfer of dark-grown angiosperm seedlings to light. The stability of the light-dependent NADPH-protochlorophyllide oxidoreductase in pine in the absence of accumulated substrate is consistent with either (1) a different mechanism of regulation of chlorophyll synthesis in gymnosperms or (2) a higher proportion of stable extra-plastidic protein reacting with the antibody to the light-dependent NADPH-protochlorophyllide oxidoreductase than is the case in angiosperms.

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Plastid differentiation and chlorophyll synthesis in cotyledons of black pine seedlings grown in the dark

Cited by 23 publications

References 4 publications

Tissue browning of in vitro cultures of Scots pine: Role of peroxidase and polyphenol oxidase

Tissue browning of in vitro cultures of Scots pine: Role of peroxidase and polyphenol oxidase

Photoactivation of Oxygen‐evolving System in Dark‐grown Spruce Seedlings

Immunodetection and photostability of NADPH-protochlorophyllide oxidoreductase in Pinus pinea L.

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