Plastics Completely Synthesized by Bacteria: Polyhydroxyalkanoates

Chen, Guoqiang

doi:10.1007/978-3-642-03287-5_2

Cited by 176 publications

(117 citation statements)

References 72 publications

(59 reference statements)

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“…In-depth reviews on these various PHA biosynthesis pathways and the enzymes involved have been provided by Chen [11], Lu et al [4], Madison and Huisman [13], and Khosravi-Darani et al [21]. Figure 3 shows the various routes of scl-PHA synthesis (pathways A to J) and mcl-PHA synthesis (pathways J to M) while Table 1 provides a summary of the enzymes involved.…”

Section: Pha Biosynthetic Pathwaysmentioning

confidence: 99%

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Start a Research on Biopolymer Polyhydroxyalkanoate (PHA): A Review

et al. 2014

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Section: Pha Biosynthetic Pathwaysmentioning

confidence: 99%

“…[7,8]. The production of various types of PHA material, their properties and downstream applications was recently reviewed by Philip et al [9], Olivera et al [10], Chen [11], and Rai et al [7].…”

Section: Introductionmentioning

confidence: 99%

Start a Research on Biopolymer Polyhydroxyalkanoate (PHA): A Review

et al. 2014

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“…It is thermoplastic and has good strength characteristics in comparison with widely used polylactides. P3HB is slowly biodegraded in an organism producing non-toxic 3-hydroxybutyric acid and, therefore, it can be used as amaterial for a wide spectrum of biomedical applications -from sutures and drug delivery systems to matrices for tissue engineering and reconstructive surgery [3][4][5][6]. However, high degree of crystallinity of P3HB (over 70%) leads to a high rigidity and brittleness of its products, and their low resistance to stretching [7,8].…”

Section: Introductionmentioning

confidence: 99%

Surface wettability and energy effects on the biological performance of poly-3-hydroxybutyrate films treated with RF plasma

Syromotina

Surmenev

Boyandin

et al. 2016

Materials Science and Engineering: C

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АbstractThe surface properties of poly-3-hydroxybutyrate (P3HB) membranes were modified using oxygen and an ammonia radio-frequency (RF, 13.56 MHz) plasma. The plasma treatment procedures used in the study only affected the surface properties, including surface topography, without inducing any significant changes in the crystalline structure of the polymer,with the exception being a power level of 250W. Thewettability of themodified P3HB surfaces was significantly increased after the plasma treatment, irrespective of the treatment procedure used. Itwas revealed that both surface chemistry and surface roughness changes caused by the plasma treatment affected surface wettability. A treatment-induced surface aging effect was observed and resulted in an increase in the water contact angle and a decrease in the surface free energy. However, the difference in the water contact angle between the polymers that had been treated for 4 weeks and the untreated polymer surfaces was still significant. A dependence between cell adhesion and proliferation and the polar component of the surface energy was revealed. The increase in the polar component after the ammonia plasma modification significantly increased cell adhesion and proliferation on biodegradable polymer surfaces compared to the untreated P3HB and the P3HB modified using an oxygen plasma.

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“…Biosynthesis and regulation of PHA 2.2 PHA can be synthesised using chemical or biological processes. The production of PHA by microorganisms leads to a higher molecular weight compared with chemical methods (Chen 2010). However, controlling the PHA structure in biological processes is difficult since this approach involves complex metabolic pathways which are strongly influenced by the conditions of the culture and media composition.…”

Section: Pha Production 212mentioning

confidence: 99%

“…In addition, 3-hydroxyacyl-CoA units can also be generated from β-oxidation of fatty acids of different chain lengths (Chen 2010). The three mayor PHA pathways are summarised in Pathway I generates 3-hydroxybutyrate in three stages through the sequential action of three enzymes.…”

Section: Pha Production 212mentioning

confidence: 99%

Composition of mixed culture PHA biopolymers and implications for downstream processing

Montano-Herrera¹

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Polyhydroxyalkanoates (PHAs) are biopolymers synthesised and accumulated by most bacteria for carbon and energy storage. They have properties and applications comparable to petrochemical thermoplastics. Although PHAs are produced at high yields using pure biological cultures, the use of mixed cultures can significantly reduce the production costs and make use of waste streams to produce environmentally sustainable materials. In order to produce mixed culture PHAs of relevance for broader industry applications it is necessary to develop the ability to tailor polymers with diversified mechanical properties through understanding and controlling the monomer composition and compositional distribution.Diverse and complex PHA polymer structures have been achieved in mixed microbial cultures using time-based feeding strategies. However, PHA monomer composition and compositional distribution in PHA random and blocky copolymers are sensitive to substrate feeding history, making more complex the prediction of final PHA content and composition.In this sense, a better understanding of the changing cell physiologies that develop in response to different feeding strategies and substrates is necessary to design optimised feeding strategies and process control algorithms for PHA production.This thesis presents for the first time a comprehensive flux characterisation of monomer development during mixed culture PHA accumulation concurrent with biomass growth using Metabolic Flux Analysis (MFA). A dynamic trend in active biomass growth and in polymer composition was observed and was consistent over replicate accumulations. Monomer (3-hydroxybutyrate and 3-hydroxyvalerate) incorporation into poly(3-hydroxybutyate-co-3-hydroxyvalerate) copolymers in a pilot scale production system was evaluated based on published models that describe polymer production during PHA accumulation. However, it was found that these existing models could not describe the fluctuations in the proportion of Additionally, thermal degradation of mixed culture PHAs during melt processing was assessed by Near-Infrared (NIR) spectroscopy coupled to Multivariate Data Analysis. It was shown that, with correct pretreatment, a copolymeric product that was much more stable to extrusion processing than commercially available PHA was produced.Overall, this thesis explores both the fundamental and applied aspects of PHA production by mixed microbial cultures with concurrent active biomass growth. The use of computational iii tools to explore and help understand the underlying metabolic processes was explored.Polymer composition seems to follow a very complex regulation processes which can be described through the incorporation of more detailed reactions in current metabolic models.Furthermore, this thesis gives further insight into the fundamental properties of the materials produced and an assessment of their potential for degradation during processing.iv

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Plastics Completely Synthesized by Bacteria: Polyhydroxyalkanoates

Cited by 176 publications

References 72 publications

Start a Research on Biopolymer Polyhydroxyalkanoate (PHA): A Review

Start a Research on Biopolymer Polyhydroxyalkanoate (PHA): A Review

Surface wettability and energy effects on the biological performance of poly-3-hydroxybutyrate films treated with RF plasma

Composition of mixed culture PHA biopolymers and implications for downstream processing

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