Plasticity of marrow-derived stem cells

Krause, Diane S.

doi:10.1038/sj.gt.3301760

Cited by 188 publications

(113 citation statements)

References 36 publications

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“…Yet research in stem cell biology may also have great impact on cardiovascular developmental biology. For example, studies of adult stem cells have indicated that stem cells may exhibit a much higher degree of plasticity than had been previously realized (Bjornson et al, 1999;Orlic et al, 2001;Zuk et al, 2001;Krause, 2002). Accordingly, the cells of the early embryo may possess a much broader potential than is often conceded.…”

Section: Stem Cell Plasticity-implications For Heart Formation In Thementioning

confidence: 99%

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Stem cells and the formation of the myocardium in the vertebrate embryo

2003

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A major goal in cardiovascular biology is to repair diseased or damaged hearts with newly generated myocardial tissue. Stem cells offer a potential source of replacement myocytes for restoring cardiac function. Yet little is known about the nature of the cells that are able to generate myocardium and the conditions they require to form heart tissue. A source of information that may be pertinent to addressing these issues is the study of how the myocardium arises from progenitor cells in the early vertebrate embryo. Accordingly, this review will examine the initial events of cardiac developmental biology for insights into the identity and characteristics of the stem cells that can be used to generate myocardial tissue for therapeutic purposes. Anat Rec Part A 276A:2-12, 2004.

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Section: Stem Cell Plasticity-implications For Heart Formation In Thementioning

confidence: 99%

“…Circulating stem cells in the adult have been shown to help replenish various tissues, such as blood vessels, the liver, and heart (Harraz et al, 2001;Anversa and Nadal-Ginard, 2002;Krause, 2002). Among higher vertebrates, blood circulation is established early during embryogenesis.…”

Section: Does the Precardiac Mesoderm Uniquely Contribute To The Myocmentioning

confidence: 99%

Stem cells and the formation of the myocardium in the vertebrate embryo

2003

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“…Stem sel embrionik tersebut merupakan sel yang belum berdiferensiasi dan bersifat pluripoten karena sel-selnya mampu membentuk semua jaringan dan organ pada tubuh dewasa (Prentice, 2003). Penelitian eksplorasi pemanfaatan stem sel embrionik sebagai alternatif terapi sel terutama pada penyakit degeneratif telah banyak dilakukan, namun penggunaan stem sel embrionik tersebut memiliki kendala dalam bidang etika kedokteran terutama jika menggunakan stem sel yang berasal dari embrio manusia.Stem sel dewasa merupakan sel yang belum berdiferensiasi yang terdapat pada jaringan, sel tersebut bersifat multipoten dan mempunyai kemampuan self renewal (Krause, 2002). Stem sel dewasa dapat ditemukan pada sumsum tulang, otak, darah tepi, gastrointestinal, folikel rambut, hati, pankreas, jantung, kornea, retina, lemak, dan otot skeletal (Prentice, 2003).…”

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POTENSI TRANSDIFERENSIASI SEL FIBROBLAS MENJADI SEL SARAF SECARA IN VITRO (Transdifferentiation Potency of Fibroblast Cell to Neuron Cell in Vitro)

Kaiin

Djuwita

2016

J Kedokt Hewan

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ABSTRAKPenelitian ini dilakukan untuk menguji potensi sel fibroblas mengalami transdiferensiasi menjadi sel saraf secara in vitro. Penyiapan newborn rat neuron conditioned medium (NBRN CM) dilakukan dengan mengkultur sel saraf yang dikoleksi dari anak tikus yang baru lahir sampai mencapai tahap konfluen, kemudian medium diganti dengan medium Dulbecco's modified eagle medium (DMEM) tanpa serum. Setelah 48 jam, medium dikoleksi sebagai NBRN CM. Sel fibroblas dikoleksi dari jaringan otot fetus tikus yang didisosiasi menggunakan tripsin. Sel fibroblas kemudian dikultur dengan 3 perlakuan medium yaitu: mDMEM + β-merkaptoetanol 0,01 mM; mDMEM + NBRN-CM 50% dan mDMEM + β-merkaptoetanol 0,01 mM + NBRN CM 50%. Sebagai kontrol, sel fibroblas dikultur dengan mDMEM ditambah newborn calf serum (NBCS) 10%. Penambahan NBRN CM ke dalam medium kultur menghasilkan sel saraf sebesar 12,97 % pada kultur sel fibroblas pasase ke-1. Penambahan β-merkaptoetanol ke dalam medium kultur meningkatkan persentase sel saraf yang ditemukan (14,60%). Hasil yang serupa terjadi pada kultur sel fibroblas pasase ke-3 (12,67%; 13,17%). Hal ini menunjukkan bahwa sel fibroblas berpotensi mengalami transdiferensiasi menjadi sel saraf pada saat diinduksi dengan medium kultur sel saraf (NBRN CM). Perlu penelitian lebih lanjut untuk melihat potensi transdiferensiasi sel fibroblas. ____________________________________________________________________________________________________________________ Kata kunci: fibroblas, transdiferensiasi, conditioned medium, sel saraf, in vitro ABSTRACT The aim of this study was to examine the potency of fibroblast cells transdifferentiated to neuron cells in vitro. Newborn rat neuron conditioned medium (NBRN CM) was collected from neuron cells cultured with mDMEM without serum for 48 hours. Fibroblast cells were collected from fetal rat muscle treated with trypsin. Fibroblast cells were culture with 3 kind of culture medium: mDMEM + 0.01 mM β-mercaptoethanol; mDMEM + 50% NBRN-CM and mDMEM + 0.01 mM β-mercaptoethanol + 50% NBRN CM . As control, cells was cultured with mDMEM +10% newborn calf serum (NBCS). The addition of NBRN CM into PENDAHULUANStem sel adalah sel yang terdapat dalam tubuh dan memiliki beberapa karakteristik seperti sel yang belum berdiferensiasi dan memiliki fungsi khusus. Berdasarkan sumbernya, stem sel dapat digolongkan menjadi stem sel embrionik dan stem sel dewasa. Stem sel embrionik terdapat pada inner cell mass (ICM) embrio tahap blastosis. Stem sel embrionik tersebut merupakan sel yang belum berdiferensiasi dan bersifat pluripoten karena sel-selnya mampu membentuk semua jaringan dan organ pada tubuh dewasa (Prentice, 2003). Penelitian eksplorasi pemanfaatan stem sel embrionik sebagai alternatif terapi sel terutama pada penyakit degeneratif telah banyak dilakukan, namun penggunaan stem sel embrionik tersebut memiliki kendala dalam bidang etika kedokteran terutama jika menggunakan stem sel yang berasal dari embrio manusia.Stem sel dewasa merupakan sel yang belum berdiferensiasi yang terdapat pada jar...

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“…Bone marrow-derived MSCs (BMSCs) have the potential for self-renewal and differentiation into several cell types after culture expansion in vitro and in vivo, including osteoblastic, chondrocytic, adipocytic and neuronal cells [3][4][5][6][7]. Differentiation into osteoblastic lineage cells after subculture in osteogenic-induced media, containing dexamethasone (Dex), ascorbic acid phosphate and β-glycerophosphate (β-GP), has been shown for rat and human BMSCs [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Increased osteogenesis with hydroxyapatite constructs combined with serially-passaged bone marrow-derived mesenchymal stem cells

Akahane

Ueha²,

Shimizu³

et al. 2012

SCD

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We have previously reported on both the osteogenic potential of hydroxyapatite (HA) combined with bone marrow-derived mesenchymal stem cells (BMSCs) and a method involving osteogenic matrix cell sheet transplantation of BMSCs. In the present study, we assessed the osteogenic potential of serially-passaged BMSCs, both in vitro and in vivo. We also assessed whether an additional cell-loading technique can regain the osteogenic potential of the constructs combined with serially-passaged BMSCs. The present study revealed that passage (P) 1 cells cultured in osteogenic-induced medium showed strong positive staining for alkaline phosphatase (ALP) and Alizarin Red S, whereas P3 cells showed faint staining for ALP, with no Alizarin Red S staining. Staining of P1, P2 and P3 cells were progressively weaker, indicating that the osteogenic potential of the serially-passaged rat BMSCs is lost after P3 in vitro. The in vivo study showed that little bone formation was observed in the HA constructs seeded with P3 cells, 4 weeks after subcutaneous implantation. However, P3 cell/HA constructs which had increased cell-loading showed abundant bone formation within the pores of the HA construct. ALP and osteocalcin mRNA expression in these constructs was significantly higher than that of constructs with regular cell-seeding. The present study indicates that the osteogenic potential of the constructs with serially-passaged BMSCs is increased by additional cell-loading. This method can be applied to cases requiring hard tissue reconstruction, where BMSCs require serial expansion of cells.

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Plasticity of marrow-derived stem cells

Cited by 188 publications

References 36 publications

Stem cells and the formation of the myocardium in the vertebrate embryo

Stem cells and the formation of the myocardium in the vertebrate embryo

POTENSI TRANSDIFERENSIASI SEL FIBROBLAS MENJADI SEL SARAF SECARA IN VITRO (Transdifferentiation Potency of Fibroblast Cell to Neuron Cell in Vitro)

Increased osteogenesis with hydroxyapatite constructs combined with serially-passaged bone marrow-derived mesenchymal stem cells

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