Plasticity of cell proliferation in the retina of Austrolebias charrua fish under light and darkness conditions

Berrosteguieta, Inés; Rosillo, Juan Carlos; Herrera, María Laura; Olivera-Bravo, Silvia; Casanova, Gabriela; Herranz, Vicente; García‐Verdugo, José Manuel; Fernández, Ana María González

doi:10.1016/j.crneur.2022.100042

Cited by 3 publications

(1 citation statement)

References 80 publications

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“…To strengthen this approach, we tested two monoclonal antibodies, Xap-2 and Xap-1, which were generated against the Xenopus retina ( Harris and Messersmith, 1992 ). The Xap-2 antigen recognizes outer segments of rods in frogs and killifish ( Choi et al, 2011 ; Berrosteguieta et al, 2022 ). The Xap-1 antigen has been mapped to Grp78 (Heat shock protein a5), which is detected in outer segments of rods and cones in frogs, but only of cones in mice, monkeys, and pigs ( Nookala et al, 2010 ).…”

Section: Resultsmentioning

confidence: 99%

The regeneration-responsive element careg monitors activation of Müller glia after MNU-induced damage of photoreceptors in the zebrafish retina

Bise

Pfefferli

Bonvin

et al. 2023

Front. Mol. Neurosci.

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In contrast to mammals, zebrafish can regenerate their damaged photoreceptors. This capacity depends on the intrinsic plasticity of Müller glia (MG). Here, we identified that the transgenic reporter careg, a marker of regenerating fin and heart, also participates in retina restoration in zebrafish. After methylnitrosourea (MNU) treatment, the retina became deteriorated and contained damaged cell types including rods, UV-sensitive cones and the outer plexiform layer. This phenotype was associated with the induction of careg expression in a subset of MG until the reconstruction of the photoreceptor synaptic layer. Single-cell RNA sequencing (scRNAseq) analysis of regenerating retinas revealed a population of immature rods, defined by high expression of rhodopsin and the ciliogenesis gene meig1, but low expression of phototransduction genes. Furthermore, cones displayed deregulation of metabolic and visual perception genes in response to retina injury. Comparison between careg:EGFP expressing and non-expressing MG demonstrated that these two subpopulations are characterized by distinct molecular signatures, suggesting their heterogenous responsiveness to the regenerative program. Dynamics of ribosomal protein S6 phosphorylation showed that TOR signaling became progressively switched from MG to progenitors. Inhibition of TOR with rapamycin reduced the cell cycle activity, but neither affected careg:EGFP expression in MG, nor prevented restoration of the retina structure. This indicates that MG reprogramming, and progenitor cell proliferation might be regulated by distinct mechanisms. In conclusion, the careg reporter detects activated MG, and provides a common marker of regeneration-competent cells in diverse zebrafish organs, including the retina.

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Section: Resultsmentioning

confidence: 99%

The regeneration-responsive element careg monitors activation of Müller glia after MNU-induced damage of photoreceptors in the zebrafish retina

Bise

Pfefferli

Bonvin

et al. 2023

Front. Mol. Neurosci.

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Rod precursors in the adult retina of the Austrolebias charrua annual fish

et al. 2023

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Daily rhythm of locomotor and reproductive activity in the annual fish Garcialebias reicherti (Cyprinodontiformes: Rivulidae)

Olivera,

Passos,

Vazquez

et al. 2024

Neotrop. ichthyol.

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Daily rhythms of behavior and their synchronization in relevant social contexts are fundamental for the survival and reproductive success of all animal species. South American annual fish are adapted to extreme environmental conditions, where the ponds they inhabit dry out as the year progresses, while engaging in reproductive behavior from sexual maturity to death. The ever-changing environmental cycles these species are subjected to makes them an excellent model for studying the expression of biological rhythms in nature. In this work we show for the first time that Garcialebias reicherti, an annual fish native to Uruguay shows daily rhythms in both their locomotor and reproductive behavior. This species shows diurnal behavioral patterns, with neither sex nor reproductive context affecting the phase relationship between the light/dark cycle and activity. However, reproductive context modulates the amount of locomotor activity and leads to synchronization between members of the dyads, while introducing a second behavioral rhythm for reproductive events. Reproductive context emerges as a significant modulator of rhythmic behavior, driving circadian rhythms synchronization alongside environmental zeitgebers, while illuminating the complexity of physiological and behavioral coordination.

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Plasticity of cell proliferation in the retina of Austrolebias charrua fish under light and darkness conditions

Cited by 3 publications

References 80 publications

The regeneration-responsive element careg monitors activation of Müller glia after MNU-induced damage of photoreceptors in the zebrafish retina

The regeneration-responsive element careg monitors activation of Müller glia after MNU-induced damage of photoreceptors in the zebrafish retina

Rod precursors in the adult retina of the Austrolebias charrua annual fish

Daily rhythm of locomotor and reproductive activity in the annual fish Garcialebias reicherti (Cyprinodontiformes: Rivulidae)

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