Plastic Compressed Collagen Constructs for Ocular Cell Culture and Transplantation: A New and Improved Technique of Confined Fluid Loss

Levis, Hannah J.; Menzel‐Severing, Johannes; Drake, Rosemary; Daniels, Julie T.

doi:10.3109/02713683.2012.725799

Cited by 38 publications

(23 citation statements)

References 23 publications

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“…To date, some of the most promising substitutes are represented by collagen‐based scaffold materials, which, once crosslinked, are shown to be usable as long‐term corneal stromal replacements (as previously reviewed) . Typically, these materials were used in a top‐down approach, with an initial step of hydrogel formation, followed by cell seeding, and matrix rearrangement after grafting . More recently, however, corneal stromal tissues have been engineered in vitro by means of bottom‐up strategies using the cells themselves to generate the matrix, i.e., comprise aligned collagen fibrils, and thus recreate the well‐defined, 3D microarchitecture of the native organ .…”

Section: Introductionmentioning

confidence: 99%

Template Curvature Influences Cell Alignment to Create Improved Human Corneal Tissue Equivalents

et al. 2017

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To accurately create corneal stromal equivalents with native‐like structure and composition, a new biofunctionalized, curved template is developed that allows the precise orientation of cells and of their extracellular matrix. This template is the first demonstration that curvature alone is sufficient to induce the alignment of human corneal stromal cells, which in turn are able to biofabricate stromal tissue equivalents with cornea‐like shape and composition. Specifically, tissues self‐released from curved templates show a highly organized nanostructure, comprised of aligned collagen fibrils, significantly higher expression of corneal stroma‐characteristic markers keratocan, lumican, decorin, ALDH3, and CHST6 (p = 0.012, 0.033, 0.029, 0.003, and 0.02, respectively), as well as significantly higher elastic modulus (p = 0.0001) compared with their planar counterparts. Moreover, curved tissues are shown to support the growth, stratification, and differentiation of human corneal epithelial cells in vitro, while maintaining their structural integrity and shape without any supporting carriers, scaffolds, or crosslinking agents. Together, these results demonstrate that corneal stromal cells can align and create highly organized, purposeful tissues by the influence of substrate curvature alone, and without the need of additional topographical cues. These findings can be important to further understand the mechanisms of corneal biosynthesis both in vitro and in vivo.

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Section: Introductionmentioning

confidence: 99%

Template Curvature Influences Cell Alignment to Create Improved Human Corneal Tissue Equivalents

et al. 2017

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“…This 3D culture matrix is based upon the concept of concentrating a cell-seeded collagen hydrogel by removing interstitial fluid [12], [13] and allows for easily reproducible, type-I collagen based, 3D cultures in a 96-well format. A neutralized collagen solution is mixed with cells and subsequently is heated to induce fibrillogenesis and encapsulate the cells in situ ( Figure S1a ).…”

Section: Introductionmentioning

confidence: 99%

Maturation of Induced Pluripotent Stem Cell Derived Hepatocytes by 3D-Culture

et al. 2014

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Induced pluripotent stem cell derived hepatocytes (IPSC-Heps) have the potential to reduce the demand for a dwindling number of primary cells used in applications ranging from therapeutic cell infusions to in vitro toxicology studies. However, current differentiation protocols and culture methods produce cells with reduced functionality and fetal-like properties compared to adult hepatocytes. We report a culture method for the maturation of IPSC-Heps using 3-Dimensional (3D) collagen matrices compatible with high throughput screening. This culture method significantly increases functional maturation of IPSC-Heps towards an adult phenotype when compared to conventional 2D systems. Additionally, this approach spontaneously results in the presence of polarized structures necessary for drug metabolism and improves functional longevity to over 75 days. Overall, this research reveals a method to shift the phenotype of existing IPSC-Heps towards primary adult hepatocytes allowing such cells to be a more relevant replacement for the current primary standard.

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“…Additionally, there is no need for preoperative modifications of the tested membranes like compressing the collagen membranes or seeding of keratocytes into the membranes, which is advantageous when compared with other materials reported in the literature 28,29 In contrast to amniotic membrane storage over a long period at room temperature is possible.…”

Section: Fig 8 (A)mentioning

confidence: 99%

“…Compressed collagen membranes with cultured corneal fibroblasts seemed to be a suitable growth substrate for limbal epithelial cells in cell culture, but have yet to be tested in vivo. 28,29 Other methods, which have been studied, introduced cross-links between the collagen fibers chemically. 30,31 So far, no in vivo experiments have been performed utilizing UV light/riboflavin cross-linked collagen membranes for the treatment of corneal defects.…”

Section: Fig 8 (A)mentioning

confidence: 99%

Novel Collagen Membranes for the Reconstruction of the Corneal Surface

Petsch

Schlötzer‐Schrehardt²,

Meyer‐Blazejewska³

et al. 2014

Tissue Engineering Part A

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No standardized biomaterial exists for the surgical treatment of persistent corneal erosions and ulcerations. We analyzed the suitability and biocompatibility of defined noncross-linked and UV/riboflavin cross-linked equine type I collagen membranes for the reconstruction of the corneal surface. Isolated human oral mucosa epithelial cells, a cell type in clinical use for the treatment of ocular surface diseases, were subcultivated on both types of membranes and examined concerning cell adhesion, proliferation, and differentiation. Biocompatibility was evaluated following superficial and intrastromal corneal transplantation in New Zealand white rabbits. In cell cultures all collagen membranes supported adhesion of oral mucosa epithelial cells leading to the formation of multilayered epithelial cell sheets. After intrastromal corneal implantation clinical signs of degradation were seen in all variants of collagen membranes, which was fastest in noncross-linked variants. The histological and ultrastructural level invasion of keratocytes and production of new collagen fibers inside the collagen membranes could be detected in noncross-linked variants. After superficial corneal implantation covering of the membranes by corneal epithelium over time was visible. Ultrastructural analysis showed a slower rate of degradation and less invading keratocytes in cross-linked variants compared with noncross-linked collagen membranes. Cross-linked and noncross-linked variants of the collagen membrane proofed to be suitable to serve as a carrier for epithelial stem cells in vitro and showed a high biocompatibility in vivo. These results indicate that the tested collagen membranes might be suitable for the reconstruction of the corneal surface in patients with nonhealing ulcerations. Whether membranes with faster or slower degradation properties are preferable for the treatment of persistent corneal ulcerations might depend on the underlying corneal pathology and the degree of concomitant inflammation.

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Plastic Compressed Collagen Constructs for Ocular Cell Culture and Transplantation: A New and Improved Technique of Confined Fluid Loss

Cited by 38 publications

References 23 publications

Template Curvature Influences Cell Alignment to Create Improved Human Corneal Tissue Equivalents

Template Curvature Influences Cell Alignment to Create Improved Human Corneal Tissue Equivalents

Maturation of Induced Pluripotent Stem Cell Derived Hepatocytes by 3D-Culture

Novel Collagen Membranes for the Reconstruction of the Corneal Surface

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