Plastibodies for multiplexed detection and sorting of microplastic particles in high-throughput

Engineered Anchor Peptide LCI with a Cobalt Cofactor Enhances Oxidation Efficiency of Polystyrene Microparticles

“…Anchor peptides (APs) are versatile adhesive peptides that can interact with a wide range of surfaces, including stainless steel and polymer surfaces [12] through multiple non‐covalent interactions such as electrostatic, polar, hydrophobic, and hydrogen bonds [13–15] . As previously demonstrated, β‐stranded anchor peptide LCI (“liquid chromatography peak I”) showed specific and efficient binding to many polymer surfaces like polystyrene under mild conditions [16–24] . As one gram of anchor peptide is sufficient to coat a surface of up to 654 m 2 as monolayer, [25] anchor peptides offer a scalable, simple and cost‐effective functionalization technology through spraying and dip coating [25–28] …”

“…Fluorescence‐based characterization was achieved by labeling the polystyrene particles with plastibodies. Plastibodies are fluorescent peptides labeled or conjugated with material‐specific binding properties that are comparable to binding properties of antibodies [24] . A Strep‐tag‐bearing fluorescence indicator Strep‐Tactin®XT DY‐549 (DY549), which showed a high affinity and specificity to the Strep‐tag, was used.…”

“…[13][14][15] As previously demonstrated, β-stranded anchor peptide LCI ("liquid chromatography peak I") showed specific and efficient binding to many polymer surfaces like polystyrene under mild conditions. [16][17][18][19][20][21][22][23][24] As one gram of anchor peptide is sufficient to coat a surface of up to 654 m 2 as monolayer, [25] anchor peptides offer a scalable, simple and cost-effective functionalization technology through spraying and dip coating. [25][26][27][28] Organometallic catalysts allow selective CÀ H bond oxidation of small molecule alkanes by various oxidants, [29][30][31][32][33][34][35][36][37][38][39][40][41] but to limited extent of polyolefins.…”

“…Plastibodies are fluorescent peptides labeled or conjugated with material-specific binding properties that are comparable to binding properties of antibodies. [24] A Strep-tag-bearing fluorescence indicator Strep-Tactin ® XT DY-549 (DY549), which showed a high affinity and specificity to the Strep-tag, was used. Characteristic absorption and emission maxima for DY549 were found at λ = 550-560 and λ = 575 nm, respectively.…”

Ein modifiziertes Ankerpeptid LCI mit einem Cobalt‐Cofaktor verbessert die Oxidationseffizienz von Polystyrol‐Mikropartikeln

“…[13][14][15] Wie bereits gezeigt wurde, bindet das β-Faltblatt-Ankerpeptid LCI ("liquid chromatography peak I") spezifisch und effizient an etliche Polymeroberflächen wie Polystyrol unter milden Bedingungen. [16][17][18][19][20][21][22][23][24] Da ein Gramm Ankerpeptid ausreicht, eine Oberfläche von bis zu 654 m 2 mit einer Monolage zu beschichten, [25] bieten Ankerpeptide eine skalierbare, einfache und kostengünstige Funktionalisierungsmethode durch Sprühen und Tauchbeschichtung. [25][26][27][28] Metallorganische Katalysatoren ermöglichen die selektive Oxidation von CÀ H-Bindungen kleiner Alkanmoleküle durch unterschiedliche Oxidationsmittel, [29][30][31][32][33][34][35][36][37][38][39][40][41] was bei Polyolefinen jedoch nur in begrenztem Umfang funktioniert.…”