Plasmonic Tipless Pyramid Arrays for Cell Poration

Courvoisier, Sébastien; Saklayen, Nabiha; Huber, Maximilian; Chen, Jun; Diebold, Eric D.; Bonacina, Luigi; Wolf, Jean‐Pierre; Mazur, Eric

doi:10.1021/acs.nanolett.5b01697

Cited by 23 publications

(26 citation statements)

References 20 publications

(49 reference statements)

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“…Plasmonic substrate-based methods operate with the same physical poration mechanism as plasmonic nanoparticles but use lithography techniques to precisely pattern a surface of nanostructures that are not ingested by the cell. Recent studies have also shown that plasmonic substrates can deliver a broad range of cargo, including fluorescent probes, plasmids, bacteria, and proteins into cells [8,18,19,33,34]. Despite providing promising intracellular delivery results, the cellular changes induced by plasmonic substrates at the single cell level are not well understood [8,18,34].…”

Section: Introductionmentioning

confidence: 99%

Analysis of poration-induced changes in cells from laser-activated plasmonic substrates

Saklayen

Kalies²,

Madrid

et al. 2017

Biomed. Opt. Express

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Laser-exposed plasmonic substrates permeabilize the plasma membrane of cells when in close contact to deliver cell-impermeable cargo. While studies have determined the cargo delivery efficiency and viability of laser-exposed plasmonic substrates, morphological changes in a cell have not been quantified. We porated myoblast C2C12 cells on a plasmonic pyramid array using a 532-nm laser with 850-ps pulse length and time-lapse fluorescence imaging to quantify cellular changes. We obtain a poration efficiency of 80%, viability of 90%, and a pore radius of 20 nm. We quantified area changes in the plasma membrane attached to the substrate (10% decrease), nucleus (5 -10% decrease), and cytoplasm (5 -10% decrease) over 1 h after laser treatment. Cytoskeleton fibers show a change of 50% in the alignment, or coherency, of fibers, which stabilizes after 10 mins. We investigate structural and morphological changes due to the poration process to enable the safe development of this technique for therapeutic applications. Brown, "Glass needle-mediated microinjection of macromolecules and transgenes into primary human blood stem/progenitor cells," Blood 95(2),

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Section: Introductionmentioning

confidence: 99%

Analysis of poration-induced changes in cells from laser-activated plasmonic substrates

Saklayen

Kalies²,

Madrid

et al. 2017

Biomed. Opt. Express

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“…However, there is still a need for a high-efficiency, high-throughput, low-toxicity, cost-effective intracellular delivery technique that is applicable to a range of cells types for a range of cargoes. Plasmonic nanostructured surfaces may be a promising alternative to the currently available intracellular delivery techniques and utilize the unique ability of plasmonic structures to absorb laser light energy and transfer the energy to a confined volume within the nearby surrounding medium [31,39,[49][50][51][52]. Upon illumination with a short laser pulse, the laser light energy is strongly absorbed by the plasmonic nanostructures, resulting in a rise in temperature [1,[57][58][59].…”

Section: Resultsmentioning

confidence: 99%

“…Laser-activated nanostructured substrates bypass this potential toxicity problem, as cells can be cultured on the substrates, porated, and removed from the substrates (which remain intact) after intracellular delivery without leaving metallic particles within the cells [31,39,[49][50][51][52]. In this thesis we explore the fabrication of various thermoplasmonic nanostructured substrates for intracellular delivery and use the fabricated substrates to deliver a wide range of membrane-impermeable cargoes (dyes, dextrans, proteins, etc.)…”

Section: Laser-mediated Cell Poration For Intracellular Deliverymentioning

confidence: 99%

Plasmonic Intracellular Delivery

Madrid¹

2018

Plasmonics

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This chapter describes the significance of plasmonics to the field of intracellular delivery. We begin by discussing the significance of intracellular delivery, its applications in biology and medicine, and the currently available intracellular delivery techniques. Next, we discuss the field of plasmonic intracellular delivery, beginning with the discovery of optoporation. In optoporation, a laser beam is tightly focused onto a cell membrane to generate a transient pore, through which membrane-impermeable cargo can enter the cell. To improve the throughput of this technique, plasmonic materials were used for their ability to efficiently absorb laser light and generate spatially confined electric fields. Here, we describe the process by which plasmonic materials absorb laser light energy and generate plasmons. These plasmons transfer their energy to their surroundings, resulting in a rise in temperature and the subsequent creation of a bubble or shockwave. Finally, we describe how the properties of plasmons and plasmon-mediated effects facilitate cell poration for intracellular delivery.

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“…Optoporation is another physical membrane poration mode that can assist nanoneedle cell penetration, by applying focused laser pulses at the interaction region of cells with plasmonic nanoparticles, [ 162,163 ] nanopatterns, [ 164–166 ] or nanotubes. [ 51,52 ] The operating principle is that the stimulus of a femtosecond‐pulsed laser will elicit surface plasmon polaritons on the nanoneedle surface, which can decay into high‐energy electrons, or “hot electrons” ( Figure a).…”

Section: Assisted Penetrationmentioning

confidence: 99%

Nanoneedle Platforms: The Many Ways to Pierce the Cell Membrane

He¹,

Hu²,

et al. 2020

Adv Funct Materials

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Establishing techniques to efficiently and nondestructively access the intracellular milieu is essential for many biomedical and scientific applications, ranging from drug delivery, to electrical recording, to biochemical detection. Cell penetration using nanoneedle arrays is currently a research focus area because it not only meets the increasing therapeutic demands of cell modifications and genome editing, but also provides an ideal platform for tracking long-term intracellular information. Although the precise mechanism driving membrane penetration by nanoneedle arrays is still unclear, the low cytotoxicity, wide range of delivered materials, diverse cell type targets, and simple material structures of nanoneedle arrays make these splendid platforms for cell access. Here, the recent progress in this field is reviewed by examining device architectures and discussing mechanisms for nanoneedle penetration, and the major studies demonstrating the most general applicability of nanoneedle arrays, typical methodologies to access the intracellular environment using nanoneedles with spontaneous or assisted penetration modes, as well as biosafety aspects are presented. This review should be valuable for deeply understanding the materials fabrication principles, device designs, cell penetration methodologies, biosafety aspects, and application strategies of nanoneedle array-based systems that are of crucial importance for the development of future practical biomedical platforms.

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Plasmonic Tipless Pyramid Arrays for Cell Poration

Cited by 23 publications

References 20 publications

Analysis of poration-induced changes in cells from laser-activated plasmonic substrates

Analysis of poration-induced changes in cells from laser-activated plasmonic substrates

Plasmonic Intracellular Delivery

Nanoneedle Platforms: The Many Ways to Pierce the Cell Membrane

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