Plasmonic staining of DNA molecules with photo-induced Ag nanoparticles monitored using dark-field microscopy

Yamamoto, Yuko S.; Hirano, Ken; Ishido, Tomomi; Yasui, Takahiro; Murase, Norio; Baba, Yoshinobu; Itoh, Tamitake

doi:10.1039/c3cp51494c

Cited by 8 publications

(3 citation statements)

References 21 publications

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“…The substrate AgHPC1_254 shows an incomplete reduction, and there is definitely an excess (similar as in AgHPC1_365) of Ag + . Additional in situ formation of nanoparticles and/or growth of the nanoparticles under the Raman laser could occur, but this effect would be expressed to a higher extent under micro‐Raman measurements.…”

Section: Resultsmentioning

confidence: 99%

Characterization of HPC‐based photoreduced SERS substrates and detection of different organic dyes

Retko

Ropret

Korošec

et al. 2018

J Raman Spectroscopy

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Silver nanoparticles were fabricated in situ in the hydroxypropyl cellulose (HPC) matrix by irradiation at different wavelengths (365 and 254 nm) following the principles of green chemistry. HPC is involved in the silver reduction process and in the stabilization as well. Characteristics of the dispersions depend on the weight ratio between silver ions and HPC, irradiation time, and energy of the incident irradiation. Interaction at higher energies leads to higher degradation of the HPC matrix, resulting also in lower pH values of the dispersion. The latter is even more pronounced when using lower initial concentrations of silver nitrate. Different formulations were tested as surface‐enhanced Raman scattering (SERS) substrates with alizarin solutions and several other probes. Different spectral responses are related to different surface availability and/or to differences in microenvironment or macroenvironment of the nanoparticles. Substrates irradiated at lower energies show superior properties in terms of stability, whereas the substrates with a lower concentration of silver ions and irradiated with higher energies are more suitable for the detection of compounds with acidic properties (such as carminic acid and lac dye) due to lower electrostatic repulsion between the compounds and nanoparticles.

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Section: Resultsmentioning

confidence: 99%

Characterization of HPC‐based photoreduced SERS substrates and detection of different organic dyes

Retko

Ropret

Korošec

et al. 2018

J Raman Spectroscopy

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“…As shown in Equation (), size has a large influence in the electromagnetic radiation field outside the nanoparticle, and larger PNP has greater light intensity and scattering spectrum with the peak located at longer wavelength. [ 172,173 ] For example, a 60 nm AuNP has green Rayleigh scattering light with spectra at 560 nm, while a 117 nm AuNP has red and stronger scattering light centered at 635 nm. [ 174 ] Based on the latter feature, Zheng and coworkers designed a target DNA induced size enlargement of AuNPs for the sensitive detection of DNA with the assistance of glucose oxidase (GOx)‐like activity of AuNPs, and achieved the identification of ssDNA with one‐base mismatch sequence, indicating the high specificity of this strategy.…”

Section: Single Plasmonic Nanoprobes In Nucleic Acid Detectionmentioning

confidence: 99%

Individual Plasmonic Nanoprobes for Biosensing and Bioimaging: Recent Advances and Perspectives

Wang

Feng

et al. 2021

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With the advent of nanofabrication techniques, plasmonic nanoparticles (PNPs) have been widely applied in various research fields ranging from photocatalysis to chemical and bio‐sensing. PNPs efficiently convert chemical or physical stimuli in their local environment into optical signals. PNPs also have excellent properties, including good biocompatibility, large surfaces for the attachment of biomolecules, tunable optical properties, strong and stable scattering light, and good conductivity. Thus, single optical biosensors with plasmonic properties enable a broad range of uses of optical imaging techniques in biological sensing and imaging with high spatial and temporal resolution. This work provides a comprehensive overview on the optical properties of single PNPs, the description of five types of commonly used optical imaging techniques, including surface plasmon resonance (SPR) microscopy, surface‐enhanced Raman scattering (SERS) technique, differential interference contrast (DIC) microscopy, total internal reflection scattering (TIRS) microscopy, and dark‐field microscopy (DFM) technique, with an emphasis on their single plasmonic nanoprobes and mechanisms for applications in biological imaging and sensing, as well as the challenges and future trends of these fields.

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“…A promising approach to achieve highly reproducible and precision nanostructures for potential use in metamaterials is based upon bottom-up manufacturing through the use of DNAbased fabrication [10][11][12][13]. DNA origami, for instance, is an example of a fabrication scheme that produces highly organized, and geometrically precise nanoscale objects [14,15].…”

Section: Introductionmentioning

confidence: 99%

Creating metamaterial building blocks with directed photochemical metallization of silver onto DNA origami templates

et al. 2018

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DNA origami can be used to create a variety of complex and geometrically unique nanostructures that can be further modified to produce building blocks for applications such as in optical metamaterials. We describe a method for creating metal-coated nanostructures using DNA origami templates and a photochemical metallization technique. Triangular DNA origami forms were fabricated and coated with a thin metal layer by photochemical silver reduction while in solution or supported on a surface. The DNA origami template serves as a localized photosensitizer to facilitate reduction of silver ions directly from solution onto the DNA surface. The metallizing process is shown to result in a conformal metal coating, which grows in height to a self-limiting value with increasing photoreduction steps. Although this coating process results in a slight decrease in the triangle dimensions, the overall template shape is retained. Notably, this coating method exhibits characteristics of self-limiting and defect-filling growth, which results in a metal nanostructure that maps the shape of the original DNA template with a continuous and uniform metal layer and stops growing once all available DNA sites are exhausted.

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Plasmonic staining of DNA molecules with photo-induced Ag nanoparticles monitored using dark-field microscopy

Cited by 8 publications

References 21 publications

Characterization of HPC‐based photoreduced SERS substrates and detection of different organic dyes

Characterization of HPC‐based photoreduced SERS substrates and detection of different organic dyes

Individual Plasmonic Nanoprobes for Biosensing and Bioimaging: Recent Advances and Perspectives

Creating metamaterial building blocks with directed photochemical metallization of silver onto DNA origami templates

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