Plasmonic silver nanoshells for drug and metabolite detection

Huang, Lin; Wan, Jingjing; Wei, Xiang; Liu, Yu; Huang, Jingyi; Sun, Xuming; Zhang, Ru; Gurav, Deepanjali D.; Vedarethinam, Vadanasundari; Li, Yan; Chen, Ruoping; Qian, Kun

doi:10.1038/s41467-017-00220-4

Cited by 178 publications

(168 citation statements)

References 72 publications

(108 reference statements)

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“…Notably, current clinical testing of CSF glucose is limited, concerning instability of the enzyme, low sensitivity of the conventional colorimetric readout, and large sample consumption (≈200 µL). [ 29 ] For comparison, our approach offers enhanced analytical stability (at least 24 h) and reduced sample consumption (20 µL) by approximately tenfold decrease. We demonstrated the potential applicability of our SERS nanoreactor for in vitro metabolic diagnosis of postoperative brain infection and monitoring of clinical therapeutic efficiency.…”

Section: Resultsmentioning

confidence: 99%

A Biomimetic Plasmonic Nanoreactor for Reliable Metabolite Detection

et al. 2020

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and the concomitant elevation of lactate in the interstitial fluids due to the enhanced glycolysis energy metabolism. [1] Accurate profiling of medically relevant metabolites in human body fluids provides molecular information for deep understanding of pathological mechanism at a more distal level than genomic analysis, and enables precise diagnosis and treatment of the underling diseases. [2] Till now, different advanced techniques, including magnetic resonance spectroscopy, [3] mass spectrometry, [4] and chromatography coupled mass spectroscopy, [5] have been developed to measure metabolites in vitro and in vivo. While useful, these techniques require sophisticated and expensive equipment, hampering their use in rapid point-ofcare clinical testing. By contrast, enzymebased bioassays, where the presence of analytes is optically or electrochemically detected through the highly specific and efficient enzymatic reaction, provide novel opportunities for detection metabolite biomarkers. [6] These approaches show enough figures of merits regarding device portability, speed, sample consumption and cost. However, the inherent instability of enzyme at elevated temperature, in circumstance containing digestive enzymes or chemical chelates, and when storing the enzyme long-term represents a longstanding yet largely unsolved challenge that leads to poor reproducibility and accuracy in enzyme-based metabolite analysis. [7] Reliable monitoring of metabolites in biofluids is critical for diagnosis, treatment, and long-term management of various diseases. Although widely used, existing enzymatic metabolite assays face challenges in clinical practice primarily due to the susceptibility of enzyme activity to external conditions and the low sensitivity of sensing strategies. Inspired by the micro/ nanoscale confined catalytic environment in living cells, the coencapsulation of oxidoreductase and metal nanoparticles within the nanopores of macroporous silica foams to fabricate all-in-one bio-nanoreactors is reported herein foruse in surface-enhanced Raman scattering (SERS)-based metabolic assays. The enhancement of catalytical activity and stability of enzyme against high temperatures, long-time storage or proteolytic agents are demonstrated. The nanoreactors recognize and catalyze oxidation of the metabolite, and provide ratiometric SERS response in the presence of the enzymatic by-product H 2 O 2 , enabling sensitive metabolite quantification in a "sample in and answer out" manner. The nanoreactor makes any oxidoreductase-responsible metabolite a candidate for quantitative SERS sensing, as shown for glucose and lactate. Glucose levels of patients with bacterial infection are accurately analyzed with only 20 µL of cerebrospinal fluids, indicating the potential application of the nanoreactor in vitro clinical testing.

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Section: Resultsmentioning

confidence: 99%

A Biomimetic Plasmonic Nanoreactor for Reliable Metabolite Detection

et al. 2020

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“…First, to assess the different sample preparation methods for metabolomic analyses, we selected the LC‐MS/MS platform. Although the plasmonic materials‐based laser desorption/ionization mass spectrometry has been developed to detect some specific metabolites, it is still challenging to use for the global intracellular metabolite profiling owing to its lower sensitivity and lower quantitative reproducibility. In contrast, the LC‐MS/MS platform, especially for the UPLC‐ESI‐MS/MS system system used in this study, has combined high sensitivity, high mass accuracy, high resolution with fast acquisition speeds, and stable mass accuracy over days of acquisition, which allows to expand the coverage of detected metabolites, especially for the low abundance metabolites.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Improvement of Sample Preparation Methodologies Facilitates Dynamic Metabolomics ofAspergillus niger

Zheng

Cairns

et al. 2018

Biotechnol. J.

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Metabolomics is an essential discipline in industrial biotechnology. Sample preparation approaches dramatically influence data quality and, ultimately, interpretation and conclusions from metabolomic experiments. However, standardized protocols for highly reproducible metabolic datasets are limited, especially for the fungal cell factory Aspergillus niger. Here, an improved liquid chromatography-tandem mass spectrometry-based pipeline for A. niger metabolomics is developed. It is found that fast filtration with liquid nitrogen is more suitable for cell quenching, causing minimal disruption to cell integrity, and improved intracellular metabolite recovery when compared to cold methanol quenching approaches. Seven solutions are evaluated for intracellular metabolite extraction, and found acetonitrile/water (1:1, v/v) at -20 °C, combined with boiling ethanol extraction protocols, showed unbiased metabolite profiling. This improved methodology is applied to unveil the dynamic metabolite profile of one citrate over-producing A. niger isolate under citrate fermentation. Citrate precursors, especially pyruvate, oxaloacetate, and malate, are maintained at a relatively high intracellular level, which can be necessary for high citrate synthesis flux. Glutamine shows a similar trend compared to citrate production, suggesting glutamine may be involved in intracellular pH homeostasis. Taken together, this study delivers a highly standardized and improved metabolomics methodology and paves the way for systems metabolic engineering in biotechnologically important fungi.

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“…Due to their tremendous scattering efficiency, structural designability, optical tunability , and surface functionalization ability , colloidal plasmonic NPs and regular nanoplasmonic structures have brought new opportunities to a number of areas and opened doors for substantial challenges . Through designing the size, shape, and composition of plasmonic NPs, various types of plasmonic NPs have been engineered with specific plasmonic properties addressing different applications .…”

Section: Introductionmentioning

confidence: 99%

Designable nanoplasmonic biomarkers for direct microscopy cytopathology diagnostics

Wang

Darviot

Zapata-Farfan

et al. 2019

Journal of Biophotonics

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Direct microscopy interpretation of fineneedle biopsy cytological samples is routinely used by practicing cytopathologists.Adding possibility to identify selective and multiplexed biomarkers on the same samples and with the same microscopy technique can greatly improve diagnostic accuracy. In this article, we propose to use biomarkers based on designable plasmonic nanoparticles (NPs) with unique optical properties and excellent chemical stability that can satisfy the above-mentioned requirements. By finely controlling the size and composition of gold-silver alloy NPs and gold nanorods, the NPs plasmonic resonance properties, such as scattering efficiency and resonance peak spectral position, are adjusted in order to provide reliable identification and chromatic differentiation by conventional direct microscopy. Efficient darkfield NPs imaging is performed by using a novel circular side illumination adaptor that can be easily integrated into any microscopy setup while preserving standard cytopathology visualization method. The efficiency of the proposed technology for fast visual detection and differentiation of three spectrally distinct NP-markers is demonstrated in different working media, thus confirming the potential application in conventional cytology preparations. It is worth emphasizing that the presented technology does not interfere with standard visualization with immunohistochemical staining, but should rather be considered as a second imaging modality to confirm the diagnostics. K E Y W O R D S

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Plasmonic silver nanoshells for drug and metabolite detection

Cited by 178 publications

References 72 publications

A Biomimetic Plasmonic Nanoreactor for Reliable Metabolite Detection

A Biomimetic Plasmonic Nanoreactor for Reliable Metabolite Detection

Comprehensive Improvement of Sample Preparation Methodologies Facilitates Dynamic Metabolomics ofAspergillus niger

Designable nanoplasmonic biomarkers for direct microscopy cytopathology diagnostics

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