Plasmonic nanorod probes’ journey inside plant cells for <i>in vivo</i> SERS sensing and multimodal imaging

Garcia, Vanessa Cupil; Li, Joy Q.; Stephen, Stephen J; Odion, Ren A.; Strobbia, Pietro; Menozzi, Luca; Ma, Chenshuo; Hu, Jianhong; Zentella, Rodolfo; Boyanov, Maxim I.; Finfrock, Y. Zou; Gürsoy, Doğa; Douglas, Deirdre Sholto; Yao, Junjie; Sun, Tai-ping; Kemner, K. M.; Vo‐Dinh, Tuan

doi:10.1039/d2nr06235f

Cited by 3 publications

(1 citation statement)

References 81 publications

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“…Moreover, SERS active NMs could be efficiently directed to the interest zone for the detection of multiple compounds (including inorganic molecules and ions [12] as well as biological analytes [13] ) and imaging in vivo applications inside cells. [14] This intracellular monitor possibility is compelling because of the multiple examples of self-guided NMs toward and inside cells that have been reported. [15] Alternatively, SERS active NMs provide an effective option to retrieve information from the surrounding media.…”

Section: Introductionmentioning

confidence: 99%

Nano/Micromotor‐Driven SERS for Highly Sensitive and Spatially Controlled Sensing

Becerril‐Castro,

Salgueiriño,

Correa‐Duarte

et al. 2023

Adv Funct Materials

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Nano/micromotors (NMs) are tiny structures capable of converting various forms of energy into mechanical motion at the micro and nanoscale. These motors operate in environments characterized by low inertia and low Reynolds numbers. The potential applications of NMs are vast, particularly in the fields of biomedicine and environmental science. One of the most intriguing developments in this field is the integration of NMs with surface‐enhanced Raman scattering (SERS) spectroscopy. SERS is a powerful analytical technique that enhances the Raman intensity of molecules, allowing for highly sensitive detection and analysis of trace amounts of substances. This integration offers highly precise and localized ultrasensing capabilities. The combination of NMs with SERS can also facilitate real‐time imaging inside living organisms. This has immense potential in chemical and cell biology and medical diagnostics and prognosis. Herein this review describes the types of NMs and their fabrication, the incorporation of plasmonic nanostructures, capable of creating strong electromagnetic fields when illuminated by light, which in turn enhances the Raman signals significantly, their applications, and their future prospects in areas such as precision medicine, environmental monitoring, and possibly even in new realms like microscale robotics and targeted therapeutics.

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Section: Introductionmentioning

confidence: 99%

Nano/Micromotor‐Driven SERS for Highly Sensitive and Spatially Controlled Sensing

Becerril‐Castro,

Salgueiriño,

Correa‐Duarte

et al. 2023

Adv Funct Materials

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An in Vivo Surface-Enhanced Raman Scattering Nanosensor for Detecting Hydrogen Peroxide in Plants under Abiotic Stresses

Tian,

Cong,

Sun

et al. 2024

ACS Appl. Nano Mater.

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Determining whether plants are under stress before obvious disease symptoms is crucial for precision agriculture. Intercellular hydrogen peroxide (H2O2) in plants can be used to indicate stress. However, most current H2O2 detection methods are destructive or limited by chlorophyllous autofluorescence. Herein, we constructed an in vivo surface-enhanced Raman scattering (SERS) sensor for nondestructive, in situ, and highly sensitive detection of H2O2 in plant leaves. The sensor with the designed size can enable nondestructive and in situ H2O2 detection without fluorescence interference. In addition, Ag decahedral nanoparticles with sharp edges and tips enhance the SERS effect, and the lowest detection concentration of H2O2 can reach 10 nM in vitro. The utility of the SERS sensor is demonstrated by Oxalis corniculata under wound, heat, and cold stresses. This SERS sensor can provide warnings before plants show visible signs of damage, thereby improving crop yields and potentially becoming an essential tool for precision agriculture.

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Contemporary applications of vibrational spectroscopy in plant stresses and phenotyping

Juárez,

Kurouski

2024

Front. Plant Sci.

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Plant pathogens, including viruses, bacteria, and fungi, cause massive crop losses around the world. Abiotic stresses, such as drought, salinity and nutritional deficiencies are even more detrimental. Timely diagnostics of plant diseases and abiotic stresses can be used to provide site- and doze-specific treatment of plants. In addition to the direct economic impact, this “smart agriculture” can help minimizing the effect of farming on the environment. Mounting evidence demonstrates that vibrational spectroscopy, which includes Raman (RS) and infrared spectroscopies (IR), can be used to detect and identify biotic and abiotic stresses in plants. These findings indicate that RS and IR can be used for in-field surveillance of the plant health. Surface-enhanced RS (SERS) has also been used for direct detection of plant stressors, offering advantages over traditional spectroscopies. Finally, all three of these technologies have applications in phenotyping and studying composition of crops. Such non-invasive, non-destructive, and chemical-free diagnostics is set to revolutionize crop agriculture globally. This review critically discusses the most recent findings of RS-based sensing of biotic and abiotic stresses, as well as the use of RS for nutritional analysis of foods.

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Plasmonic nanorod probes’ journey inside plant cells for in vivo SERS sensing and multimodal imaging

Abstract: Nanoparticle-based platforms are gaining strong interest in plant biology and bioenergy research to monitor and control biological processes in whole plants. However, in vivo monitoring of biomolecules using nanoparticles inside...

Cited by 3 publications

References 81 publications

Nano/Micromotor‐Driven SERS for Highly Sensitive and Spatially Controlled Sensing

Nano/Micromotor‐Driven SERS for Highly Sensitive and Spatially Controlled Sensing

An in Vivo Surface-Enhanced Raman Scattering Nanosensor for Detecting Hydrogen Peroxide in Plants under Abiotic Stresses

Contemporary applications of vibrational spectroscopy in plant stresses and phenotyping

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