Plasmonic Nanohole Sensor for Capturing Single Virus‐Like Particles toward Virucidal Drug Evaluation

Jackman, Joshua A.; Linardy, Eric; Yoo, Daehan; Seo, Jeongmin; Ng, Wee Keong; Klemme, Daniel J.; Wittenberg, Nathan J.; Oh, Sang‐Hyun; Cho, Nam‐Joon

doi:10.1002/smll.201501914

Cited by 64 publications

(56 citation statements)

References 59 publications

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“…It requires expensive and slow patterning techniques such as electron beam lithography (EBL) (Gordon et al 2010) and nanoimprint lithography (NIL) (Zhu et al 2015) which is not easy for mass production and is not desired for simple and disposable detection instruments at POC. More importantly, our technique shows much higher sensitivity and selectivity than nanoplasmonic sensing and other amplification-free techniques by orders of magnitude (Gau et al 2005; Henihan et al 2016; Jackman et al 2015; Klamp et al 2013; Stockman 2015; Tao et al 2015). …”

Section: Discussionmentioning

confidence: 96%

“…Other amplification-free techniques such as nanoplasmonic sensing have been used to detect virus targets by measuring the changes of the permittivity of the dielectric adjacent to the metal nanostructures caused by binding (Jackman et al 2015; Stockman 2015). This requires that the capture probe be immobilized on the metal surfaces, which limits the total capture surface area.…”

Section: Discussionmentioning

confidence: 99%

“…A number of amplification-free techniques such as plasmonic sensing (Jackman et al 2015; Stockman 2015), electrochemical detection (Gau et al 2005; Henihan et al 2016), and advanced microscopy (Klamp et al 2013; Tao et al 2015) have been introduced to achieve sensitive detection of nucleic acid targets diagnostic of pathogens. Plasmonic sensing, relying on the spectral shift of plasmonic resonances, requires complicated fabrication techniques of noble metal nanostructures, which makes it non-ideal for low-cost and disposable devices.…”

Section: Introductionmentioning

confidence: 99%

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Multiplexed efficient on-chip sample preparation and sensitive amplification-free detection of Ebola virus

Cai

Park

et al. 2017

Biosensors and Bioelectronics

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An automated microfluidic sample preparation multiplexer (SPM) has been developed and evaluated for Ebola virus detection. Metered air bubbles controlled by microvalves are used to improve bead-solution mixing thereby enhancing the hybridization of the target Ebola virus RNA with capture probes bound to the beads. The method uses thermally stable 4-formyl benzamide functionalized (4FB) magnetic beads rather than streptavidin coated beads with a high density of capture probes to improve the target capture efficiency. Exploiting an on-chip concentration protocol in the SPM and the single molecule detection capability of the antiresonant reflecting optical waveguide (ARROW) biosensor chip, a detection limit of 0.021 pfu/mL for clinical samples is achieved without target amplification. This RNA target capture efficiency is two orders of magnitude higher than previous results using streptavidin beads and the limit of detection (LOD) improves 10X. The wide dynamic range of this technique covers the whole clinically applicable concentration range. In addition, the current sample preparation time is ~1 hour which is eight times faster than previous work. This multiplexed, miniaturized sample preparation microdevice establishes a key technology that intended to develop next generation point-of-care (POC) detection system.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multiplexed efficient on-chip sample preparation and sensitive amplification-free detection of Ebola virus

Cai

Park

et al. 2017

Biosensors and Bioelectronics

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“…As the matter of fact, surface plasmon resonance (SPR) sensing is a promising label free and one-step virus detection method and has been used in rapid viral particle detection such as SARS/MERS 19 , H1N1 20 , H7N9 21 .... Once the viral particles are captured by the monoclonal antibody immobilized on the SPR sensor chip surface, the plasmon resonance wavelength or intensity change induced by the virus particle presence can be measured by an optical sensing system [22][23][24] . However conventional SPR testing equipment are bulky and not affordable to most research and clinical institutions especially in developing countries and resources limited settings 25,26 .…”

Section: Introductionmentioning

confidence: 99%

One-Step Rapid Quantification of SARS-CoV-2 Virus Particles via Low-Cost Nanoplasmonic Sensors in Generic Microplate Reader and Point-of-Care Device

Huang

Ding

Zhou

et al. 2020

Preprint

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The spread of SARS-CoV-2 virus in the ongoing global pandemics has led to infections of millions of people and losses of many lives. The rapid, accurate and convenient SARS-CoV-2 virus detection is crucial for controlling and stopping the pandemics. Diagnosis of patients in the early stage infection are so far limited to viral nucleic acid or antigen detection in human nasopharyngeal swab or saliva samples.Here we developed a method for rapid and direct optical measurement of SARS-CoV-2 virus particles in one step nearly without any sample preparation using a spike protein specific nanoplasmonic resonance sensor. We demonstrate that we can detect as few as 30 virus particles in one step within 15 minutes and can quantify the virus concentration linearly in the range of 10 3 vp/ml to 10 6 vp/ml. Measurements shown on both generic microplate reader and a handheld smartphone connected device suggest that our low-cost and rapid detection method may be adopted quickly under both regular clinical environment and resource-limited settings.

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“…19 They have also been utilized for on-chip detection and analysis of complex bioparticles such as exosomes, 20 viruses, 21 and virus-like particles. 22, 23 However, these platforms have also primarily relied on non-directional capture of molecules and particles, though some examples have spatially-heterogeneous surface chemistry for selective capture of particles. Nanoholes fabricated in free-standing silicon nitride (Si 3 N 4 ) films have been shown to be efficient at directing the flow of solution through them, which can be used for directed trapping of particles suspended in solution.…”

Section: Introductionmentioning

confidence: 99%

Nanohole Array-Directed Trapping of Mammalian Mitochondria Enabling Single Organelle Analysis

et al. 2015

Self Cite

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We present periodic nanohole arrays fabricated in free-standing metal-coated nitride films as a platform for trapping and analyzing single organelles. When a microliter-scale droplet containing mitochondria is dispensed above the nanohole array, the combination of evaporation and capillary flow directs individual mitochondria to the nanoholes. Mammalian mitochondria arrays were rapidly formed on chip using this technique without any surface modification steps, microfluidic interconnects or external power sources. The trapped mitochondria were depolarized on chip using an ionophore with results showing that the organelle viability and behavior were preserved during the on-chip assembly process. Fluorescence signal related to mitochondrial membrane potential was obtained from single mitochondria trapped in individual nanoholes revealing statistical differences between the behavior of polarized vs. depolarized mammalian mitochondria. This technique provides a fast and stable route for droplet-based directed localization of organelles-on-a-chip with minimal limitations and complexity, as well as promotes integration with other optical or electrochemical detection techniques.

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Plasmonic Nanohole Sensor for Capturing Single Virus‐Like Particles toward Virucidal Drug Evaluation

Cited by 64 publications

References 59 publications

Multiplexed efficient on-chip sample preparation and sensitive amplification-free detection of Ebola virus

Multiplexed efficient on-chip sample preparation and sensitive amplification-free detection of Ebola virus

One-Step Rapid Quantification of SARS-CoV-2 Virus Particles via Low-Cost Nanoplasmonic Sensors in Generic Microplate Reader and Point-of-Care Device

Nanohole Array-Directed Trapping of Mammalian Mitochondria Enabling Single Organelle Analysis

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