Plasmonic materials and manufacturing methods for rapid and sustainable thermal cycler for PCR

Shrestha, Kiran; Kim, Seongryeong; Cho, Gyoujin

doi:10.1016/j.mtadv.2023.100420

Materials Today Advances

2023

DOI: 10.1016/j.mtadv.2023.100420

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Plasmonic materials and manufacturing methods for rapid and sustainable thermal cycler for PCR

Kiran Shrestha,

Seongryeong Kim,

Gyoujin Cho

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2024

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Rapid quantitative PCR equipment using photothermal conversion of Au nanoshell

Ahn,

Park,

Jeong

et al. 2024

Sci Rep

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The emergence of infectious diseases worldwide necessitates rapid and precise diagnostics. Using gold nanoshells in the PCR mix, we harnessed their unique photothermal properties in the near-infrared regime to attain efficient heating, reaching ideal photothermal PCR cycle temperature profile. Our photothermal PCR method expedited DNA amplification while retaining its detection sensitivity. Combining photothermal quantitative PCR with real-time fluorometry and non-invasive temperature measurement, we could amplify the target DNA within just 25 min, with a minimum detectable DNA amount of 50 picograms. This innovation in photothermal qPCR, leveraging the photothermal properties of gold nanoshells, will pave the way for immediate point-of-care diagnostics of nucleic acid biomarkers.

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Rapid quantitative PCR equipment using photothermal conversion of Au nanoshell

Ahn,

Park,

Jeong

et al. 2024

Sci Rep

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Plasmonic materials and manufacturing methods for rapid and sustainable thermal cycler for PCR

Cited by 1 publication

References 207 publications

Rapid quantitative PCR equipment using photothermal conversion of Au nanoshell

Rapid quantitative PCR equipment using photothermal conversion of Au nanoshell

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