Plasmonic droplet screen for single-cell secretion analysis

Wei, Shih-Chung; Hsu, Myat Noe; Chen, Chia-Hung

doi:10.1016/j.bios.2019.111639

Cited by 27 publications

(12 citation statements)

References 34 publications

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“…If higher throughputs are required, droplet-based microfluidic systems provide a clear advantage over nanowell- and valve-based architectures as they can process millions of cells per hour. , Additionally, droplet-based systems have emerged that can be integrated with NGS to measure proteins via labeling with DNA-functionalized antibodies such as proximity ligation assay (PLA) and CITE-seq Valve-based architectures, on the other hand, have proved useful for long-term live-cell proteomics for studying temporal dynamics. , Recently, these microfluidic architectures have also been combined with ultrasensitive nanoplasmonic or surface-enhanced Raman spectroscopy (SERS)-based immunoassays that harness the interaction of light with metallic nanostructures for signal amplification. For example, Altug and co-workers have developed nanoplasmonic microarrays for the detection of single cell secretions. , Similarly, droplets have been used in conjunction with antibody conjugated metallic nanoparticles for cell secretion analysis. − Another method called single-cell plasmonic immunosandwich assay (scPISA) reported by Liu et al used probe-based microextraction from single cells and plasmon-enhanced Raman scattering for ultrasensitive detection of low copy number proteins . Notably, single cell proteomic analysis is becoming increasingly crucial for investigating the role of cellular heterogeneity in determining therapeutic efficacy.…”

Section: Methodsmentioning

confidence: 99%

Integrating Micro and Nano Technologies for Cell Engineering and Analysis: Toward the Next Generation of Cell Therapy Workflows

et al. 2022

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The emerging field of cell therapy offers the potential to treat and even cure a diverse array of diseases for which existing interventions are inadequate. Recent advances in micro and nanotechnology have added a multitude of single cell analysis methods to our research repertoire. At the same time, techniques have been developed for the precise engineering and manipulation of cells. Together, these methods have aided the understanding of disease pathophysiology, helped formulate corrective interventions at the cellular level, and expanded the spectrum of available cell therapeutic options. This review discusses how micro and nanotechnology have catalyzed the development of cell sorting, cellular engineering, and single cell analysis technologies, which have become essential workflow components in developing cell-based therapeutics. The review focuses on the technologies adopted in research studies and explores the opportunities and challenges in combining the various elements of cell engineering and single cell analysis into the next generation of integrated and automated platforms that can accelerate preclinical studies and translational research.

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Section: Methodsmentioning

confidence: 99%

Integrating Micro and Nano Technologies for Cell Engineering and Analysis: Toward the Next Generation of Cell Therapy Workflows

et al. 2022

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“…The protein secretion has also been studied by SPR-based strategies which allow label-free, real-time detection of molecular interactions occurring on or near a noble metal surface by monitoring changes in refractive index induced by molecular binding. − For example, Choi et al reported an immunoplasmonic approach for transforming growth factor-β (TGF-β) (Figure B) . The method used antibody-conjugated single gold nanoparticles as optical detection probes.…”

Section: Approaches To Single Cellmentioning

confidence: 99%

“…The method was capable of detecting TGF-β with picomolar sensitivity and monitoring the extracellular TGF-β level secreted from cells under stress conditions . In another work, two antibody-modified gold nanorods, which showed distinct SPR peaks, were used to create a plasmonic microdroplet platform for label-free, real-time, high-throughput, and multiplexed monitoring of the secretion of IL-8 and VEGF from single cancer cells . Besides spectral analysis, SPR imaging has also been employed for real-time monitoring of protein secretion from single cells. − These advanced methodologies not only expand the assay throughput but also bring an improvement for high spatiotemporal measurement.…”

Section: Approaches To Single Cellmentioning

confidence: 99%

Optical Sensing Strategies for Probing Single-Cell Secretion

et al. 2022

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Measuring cell secretion events is crucial to understand the fundamental cell biology that underlies cell–cell communication, migration, proliferation, and differentiation. Although strategies targeting cell populations have provided significant information about live cell secretion, they yield ensemble profiles that obscure intrinsic cell-to-cell variations. Innovation in single-cell analysis has made breakthroughs allowing accurate sensing of a wide variety of secretions and their release dynamics with high spatiotemporal resolution. This perspective focuses on the power of single-cell protocols to revolutionize cell-secretion analysis by allowing real-time and real-space measurements on single live cell resolution. We begin by discussing recent progress on single-cell bioanalytical techniques, specifically optical sensing strategies such as fluorescence-, surface plasmon resonance-, and surface-enhanced Raman scattering-based strategies, capable of in situ real-time monitoring of single-cell released ions, metabolites, proteins, and vesicles. Single-cell sensing platforms which allow for high-throughput high-resolution analysis with enough accuracy are highlighted. Furthermore, we discuss remaining challenges that should be addressed to get a more comprehensive understanding of secretion biology. Finally, future opportunities and potential breakthroughs in secretome analysis that will arise as a result of further development of single-cell sensing approaches are discussed.

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“…Apart from intracellular sensor and imaging, it is inevitable to employ single-cell secretion analysis to elucidate the heterogeneity of cellular functionalities. A one-step washing-free approach was developed for multiplex detection of single-cell secretions utilizing plasmonic droplet screen [66]. Individual cells were encapsulated with anti-body-modified gold nanorods (Au NRs) in droplets.…”

Section: Dark-field Scattering Spectrummentioning

confidence: 99%

Recent Advances in Plasmonic Nanostructures Applied for Label‐free Single‐cell Analysis

Liao

Tan

et al. 2021

Electroanalysis

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Cellular heterogeneity presents a major challenge in understanding the relationship between cells of particular genotype and response in disease. In order to characterize the cell‐to‐cell differences during the biochemical processes, single‐cell analysis is necessary. Profiting from the unique localized surface plasmon resonance (LSPR) and Mie scattering, plasmonic nanostructures have revealed stable and adjustable scattering signals, avoiding photobleaching, blinking and autofluorescence phenomenon. These characterizations are propitious to the dynamic trace and biological image of single living cells. In this review, we discuss the recent advances in plasmonic nanostructures applied for label‐free detection and monitoring of target cells at single‐cell level by using three different techniques, surface‐enhanced Raman scattering (SERS), surface‐enhanced Infrared absorption spectroscopy (SEIRAS), and dark‐field microscopy. Various avenues to design plasmonic probes combining spectra and imaging for single‐cell analysis are demonstrated as well. We hope this review can highlight the superiority of plasmonic nanostructures in single cellular analysis, and further motivate the development of label‐free cell analysis technique to elucidate cellular diversity and heterogeneity.

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Plasmonic droplet screen for single-cell secretion analysis

Cited by 27 publications

References 34 publications

Integrating Micro and Nano Technologies for Cell Engineering and Analysis: Toward the Next Generation of Cell Therapy Workflows

Integrating Micro and Nano Technologies for Cell Engineering and Analysis: Toward the Next Generation of Cell Therapy Workflows

Optical Sensing Strategies for Probing Single-Cell Secretion

Recent Advances in Plasmonic Nanostructures Applied for Label‐free Single‐cell Analysis

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