Plasmonic Circular Dichroism Study of Gold Nanorod-Quadruplex Nanobioconjugates

Abstract

Abstract:Circular dichroism spectroscopy is a simple way to study G-quadruplex structure and is very useful for monitoring the conformational changes in G-quadruplex structure induced by modifications of the environment. Plasmonic nanoparticles with localized surface plasmon resonance (LSPR) can create strong electromagnetic fields at the surface of plasmonic metals, which remarkably influence the optical properties of molecules. Plasmonic CD is a new CD signal which originates from the dipole-dipole interactions between surface plasmons of NPs and chiral biomolecules have received interest research in various fields of nanotechnology. In this paper we study the interaction between gold nanorods (GNR) and two types of G-quadruplex (parallel and antiparallel), to monitor the alterations in DNA conformation and plasmonic CD signal upon formation of GNR-quadruplex nanobioconjugate. The results from this study indicate the plasmonic CD signals in visible regions are more sensitive than Far-UV CD signals to detect the spatial conformational state of G-quadruplex-GNR nanobioconjugate.

Biomolecule-Based Optical Metamaterials: Design and Applications

“…Diversity of nanostructures or material hybridized with DNA have been characterized with an increase of CD signal in the visible region. For example, AuNR with folded G-quadruplex, ssDNAs attached on the surface of Au/Ag nanocubes, cyanine dyes positioned on NP surface through DNA-templated molecular self-assembly [ 174 , 175 , 176 ]. Additionally, the fabrication of a light-driven plasmonic nanosystem, able to translate the molecular motion into reversible chiroptical was fabricated by combining azobenzene with DNA molecules [ 17 ].…”

Progress and perspective on chiral plasmonic nanostructures enabled by DNA programming methodology

“…Bagheri et al 99 investigated the conjugates of AuNR with folded G-quadruplex and found the intensity increase of CD signal in the visible region. Lu et al 100 obtained a hybrid dandelion-like structure in which ssDNAs were attached on the surface of Au@Ag core/shell nanocubes and radially arranged owing to the electrostatic repulsions (Fig.…”

An Improved Escherichia Coli Bacterium Detection in Microchannel Based on Dielectrophoresis Impedance Measurements

“…It is an innovative field at the intersection of engineering, physics, biochemistry, nanotechnology, and biotechnology, with practical applications in the design of low volume systems of fluid. Microfluidics is used in the development of inkjet printers, DNA chips, micro-propulsion, and micro-thermal technologies [24][25][26][27][28][29][30][31].…”