Plasmonic Ag nanocube enhanced SERS biosensor for sensitive detection of oral cancer DNA based on nicking endonuclease signal amplification and heated electrode

Liu, Yan; Wu, Shao-Hua; Du, Xing-Yuan; Sun, Jian‐Jun

doi:10.1016/j.snb.2021.129854

Cited by 39 publications

(31 citation statements)

References 43 publications

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“…In most cases, raw saliva is the only biofluid that has been analyzed to clinically validate the developed biosensor. However, there are studies where the authors decided to evaluate the device performance in both artificial and real saliva, in order to estimate the matrix effect of a real biofluid as opposed to the artificial one [100][101][102][103][104][105][106][107]. Biosensing for salivary diagnostics still being a rather burgeoning research field, it is of great importance also to validate the obtained results against an established gold standard, such as ELISA.…”

Section: Biosensors and Bioelectronic Platforms For The Detection Of Oral Cancer Biomarkers In Salivamentioning

confidence: 99%

“…A variety of detection mechanisms have been employed in biosensing, including electrochemical [111][112][113]115,116,118,119,121,122,125,129,133,135,141], photoelectrochemical [114], optical [100,110,117,124,126,127,140], and mass-based systems [131]. All of the above biosensing methods enable detection with high sensitivity, and enhanced specificity [142].…”

Section: Biosensors and Bioelectronic Platforms For The Detection Of Oral Cancer Biomarkers In Salivamentioning

confidence: 99%

“…In this regard, the use of nanostructured materials is often sought to increase the sensitivity of the device. Liu et al [100] developed a biosensing platform (Figure 7a) based on plasmonic Ag nano-cubes (AgNCs) as a strategy to enhance the SERS signal. The platform combines nicking endonuclease assisted signal amplification (NESA) with heated electrodes for the detection of a DNA sequence related to oral cancer.…”

Section: Optical Biosensorsmentioning

confidence: 99%

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Malignancies and Biosensors: A Focus on Oral Cancer Detection through Salivary Biomarkers

et al. 2021

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Oral cancer is among the deadliest types of malignancy due to the late stage at which it is usually diagnosed, leaving the patient with an average five-year survival rate of less than 50%. The booming field of biosensing and point of care diagnostics can, in this regard, play a major role in the early detection of oral cancer. Saliva is gaining interest as an alternative biofluid for non-invasive diagnostics, and many salivary biomarkers of oral cancer have been proposed. While these findings are promising for the application of salivaomics tools in routine practice, studies on larger cohorts are still needed for clinical validation. This review aims to summarize the most recent development in the field of biosensing related to the detection of salivary biomarkers commonly associated with oral cancer. An introduction to oral cancer diagnosis, prognosis and treatment is given to define the clinical problem clearly, then saliva as an alternative biofluid is presented, along with its advantages, disadvantages, and collection procedures. Finally, a brief paragraph on the most promising salivary biomarkers introduces the sensing technologies commonly exploited to detect oral cancer markers in saliva. Hence this review provides a comprehensive overview of both the clinical and technological advantages and challenges associated with oral cancer detection through salivary biomarkers.

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Section: Biosensors and Bioelectronic Platforms For The Detection Of Oral Cancer Biomarkers In Salivamentioning

confidence: 99%

Section: Biosensors and Bioelectronic Platforms For The Detection Of Oral Cancer Biomarkers In Salivamentioning

confidence: 99%

Section: Optical Biosensorsmentioning

confidence: 99%

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Malignancies and Biosensors: A Focus on Oral Cancer Detection through Salivary Biomarkers

et al. 2021

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“…The SERS tags engineered by these Raman reporters are extensively applied for in vitro bioanalysis. For example, the Raman reporter of 4-mercaptobenzoic acid (4-MBA) assembled on the surface of Ag nanocube was used as SERS tag for sensitive detection of oral cancer DNA 38 . SERS tags prepared by modifying AuAgNPs with a Raman reporter molecule of 4-mercaptobenzonitrile (4-MBN) were used for multiple cancer-associated miRNAs 39 .…”

Section: Introductionmentioning

confidence: 99%

SERS Tags for Biomedical Detection and Bioimaging

Liu¹,

Gao²,

Xu³

et al. 2022

Theranostics

129

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Surface-enhanced Raman scattering (SERS) has emerged as a valuable technique for molecular identification. Due to the characteristics of high sensitivity, excellent signal specificity, and photobleaching resistance, SERS has been widely used in the fields of environmental monitoring, food safety, and disease diagnosis. By attaching the organic molecules to the surface of plasmonic nanoparticles, the obtained SERS tags show high-performance multiplexing capability for biosensing. The past decade has witnessed the progress of SERS tags for liquid biopsy, bioimaging, and theranostics applications. This review focuses on the advances of SERS tags in biomedical fields. We first introduce the building blocks of SERS tags, followed by the summarization of recent progress in SERS tags employed for detecting biomarkers, such as DNA, miRNA, and protein in biological fluids, as well as imaging from in vitro cell, bacteria, tissue to in vivo tumors. Further, we illustrate the appealing applications of SERS tags for delineating tumor margins and cancer diagnosis. In the end, perspectives of SERS tags projecting into the possible obstacles are deliberately proposed in future clinical translation.

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“…Therefore, compared with other metal nanocrystals silver nanocrystals and exhibit weaker plasma damping and greater light scattering, so they are easier to resonate. Using gold nanoparticles or graphene as deposition matrix, silver deposition has been used for the detection of biomarker antigen, DNA and RNA, as well as bacteria (Lin et al, 2014;Liu et al, 2021). In this work, the silver nanorods have become the preferred experimental objects for studying the characteristics of surface plasmon resonance.…”

Section: Introductionmentioning

confidence: 99%

Optimization Based on the Surface Plasmon Optical Properties of Adjustable Metal Nano-Microcavity System for Biosensing

et al. 2021

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This paper mainly studies the plasma optical properties of the silver nanorod and gold film system with gap structure. During the experiment, the finite element analysis method and COMSOL Multiphysics are used for modeling and simulation. The study changes the thickness of the PE spacer layer between the silver nanorod and the gold film, the conditions of the incident light and the surrounding environment medium. Due to the anisotropic characteristics of silver nanorod, the microcavity system is extremely sensitive to the changes of internal and external conditions, and the system exhibits strong performance along the long axis of the nanorod. By analyzing the extinction spectrum of the nanoparticle and the electric field section diagrams at resonance peak, it is found that the plasma optical properties of the system greatly depend on the gap distance, and the surrounding electric field of the silver nanorod is confined in the gap. Both ends of the nanorod and the gap are distributed with high concentrations of hot spots, which reflects the strong hybridization of multiple resonance modes. Under certain excitation conditions, the plasma hybridization behavior will produce a multi-pole mode, and the surface electric field distribution of the nanorod reflects the spatial directionality. In addition, the system is also highly sensitive to the environmental media, which will cause significant changes in its optical properties. The plasma microcavity system with silver nanorod and gold film studied in this paper can be used to develop high-sensitivity biosensors, which has great value in the field of biomedical detection.

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Plasmonic Ag nanocube enhanced SERS biosensor for sensitive detection of oral cancer DNA based on nicking endonuclease signal amplification and heated electrode

Cited by 39 publications

References 43 publications

Malignancies and Biosensors: A Focus on Oral Cancer Detection through Salivary Biomarkers

Malignancies and Biosensors: A Focus on Oral Cancer Detection through Salivary Biomarkers

SERS Tags for Biomedical Detection and Bioimaging

Optimization Based on the Surface Plasmon Optical Properties of Adjustable Metal Nano-Microcavity System for Biosensing

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