Plasmon properties of multilayer albumin/gold hybrid nanoparticles

Abstract

En primer lugar, quiero agradecer a la UNQ y al IMBICE, por haberme brindado un lugar de trabajo. A CONICET por la beca otorgada para realizar el doctorado. A la Agencia Internacional de Energía Atómica (IAEA) y la UNQ por el financiamiento económico. A los jurados por tomarse el trabajo de leer esta tesis. En el ámbito académico, le debo un profundo agradecimiento al Dr. Mariano Grasselli, mi director y guía profesional, el cual ha sabido enseñarme a pensar y a tratar de ser una buena profesional. Gracias por la oportunidad de formar parte de su grupo de Investigación y por la paciencia. A mi co-directora, Dra. Silvia Alonso, por sus aportes constantes a esta tesis. A mis compañeros/amigos de trabajo, con los cuales he pasado momentos inolvidables. Gracias a Silvi, Lean,

Enhanced Gold Nanoparticle-Tumor Cell Recognition by Albumin Multilayer Coating

“…2). BioHNPs spectrum clearly showed an absorption peak at 280 nm corresponding to the albumin coating and a maximum plasmon shift to 530 nm produced by the protein coating [36]. Theoretical simulations of Mie theory considering a steady coating model have explained the changes in the plasmon extinction.…”

“…The crosslinking reaction is based on the protein macro radical recombinations generated by solvent radiolysis [41]. Albumin-NPs are generated simultaneously in addition to bioHNPs [36]. Therefore, a purification protocol including centrifuging and size-exclusion chromatography steps was required to prepare a homogenous and stable batch of bioHNPs.…”

“…Theoretical simulations of Mie theory considering a steady coating model have explained the changes in the plasmon extinction. In this model, all NPs will have the same coating thickness for an experimental condition, regardless of the naked NP radii [36]; therefore, the coating process did not increase the heterogeneity of the NP size population. Dynamic light scattering (DLS) measurements of MNPs and bioHNPs showed a mean hydrodynamic diameter (HD) of 28.02 +/-0.6 nm and 77.4 +/-0.7 nm, respectively (Fig.…”

“…Synthesis of gold nanoparticles: AuNPs were prepared according to the Frens method [35]. The concentration of AuNPs was determined through UV-vis spectroscopy, according to Flores et al [36].…”

IAEA Contribution to Nanosized Targeted Radiopharmaceuticals for Drug Delivery

“…Radiation-based synthesis for NPs construction can be carried out through degradation, crosslinking, graft copolymerization, and also radiation-induced reduction of metallic ions. This technique can be used for generating (i) radiation-crosslinked nanogels from synthetic/biopolymers [ 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 ], (ii) conjugated NPs from size-controlled polymer templates through radiation [ 85 , 86 ], (iii) NPs from self-assembly of radiation-induced grafted copolymers [ 87 , 88 ], and (iv) AuNPs-hybrid NPs from radiation-induced reduction [ 89 , 90 , 91 , 92 , 93 , 94 ]. These are summarized in Table 3 .…”