Plasmon-Enhanced Photocleaving Dynamics in Colloidal MicroRNA-Functionalized Silver Nanoparticles Monitored with Second Harmonic Generation

Abstract

The photocleaving dynamics of colloidal micro-RNA-functionalized nanoparticles are studied using time-dependent second harmonic generation (SHG) measurements. Model drug-delivery systems composed of oligonucleotides attached to either silver nanoparticles or polystyrene nanoparticles using a nitrobenzyl photocleavable linker are prepared and characterized. The photoactivated controlled release is observed to be most efficient on resonance at 365 nm irradiation, with pseudo-first-order rate constants that are linearly proportional to irradiation powers. Additionally, silver nanoparticles show a 6-fold plasmon enhancement in photocleaving efficiency over corresponding polystyrene nanoparticle rates, while our previous measurements on gold nanoparticles show a 2-fold plasmon enhancement compared to polystyrene nanoparticles. Characterizations including extinction spectroscopy, electrophoretic mobility, and fluorimetry measurements confirm the analysis from the SHG results. The real-time SHG measurements are shown to be a highly sensitive method for investigating plasmon-enhanced photocleaving dynamics in model drug delivery systems.

Combinatorial Delivery of miRNA‐Nanoparticle Conjugates in Human Adipose Stem Cells for Amplified Osteogenesis

“…Representative SHG spectra, centered at 400 nm with a full‐width half‐maximum of approximately 4.5 nm, of SNP‐miR‐21 complex are shown in Figure A. The miRNA‐functionalized nanoparticles have higher SHG intensities than the corresponding nanoparticles without miRNA functionalization due to the increased surface charge density through the χ (3) effect, in agreement with our previous results . Upon irradiation at 400 nm, the SNP absorbed energy at the plasmon resonance, leading to the cleaving of the linker and release of miRNA mimic, resulting in a lower SHG intensity, as shown in Figure A.…”

“…SHG is dipole forbidden in centrosymmetric media, like bulk solution, but can be generated at the surface of nanoparticles where the symmetry is broken. Recently, SHG has been used as an accurate method to determine molecular adsorption and chemical reactions at nanoparticle surfaces, ion‐transport processes in liposomes, and the release of oligonucleotides from the surface of plasmonic nanoparticles …”

“…From the aforementioned drug vehicles, plasmonic nanoparticles have acquired considerable interest. Silver and gold nanoparticles have been investigated for siRNA delivery, specifically for their release kinetics as well as in the delivery of miR‐148b to induce osteogenesis in hASCs.…”

Green Synthesized Silver Nanoparticles of Myrtus communis L (AgMC) Extract Inhibits Cancer Hallmarks via Targeting Aldose Reductase (AR) and Associated Signaling Network

“…The size and morphology of synthesized metallic nanoparticles can be controlled with chemical reducing agents [1][2][3]. However, the reducing agents and solvents used in the synthesis of nanoparticles are hazardous or toxic to the environment [2]. Consequently, the green synthesis of nanoparticles is anticipated, even though there are a several categories of green reductants (based on isolation/extraction sources) existing in nature.…”

“…Consequently, the green synthesis of nanoparticles is anticipated, even though there are a several categories of green reductants (based on isolation/extraction sources) existing in nature. Under these circumstances, green chemistry approaches can significantly assist with the preparations of metal nanoparticles [2]. In recent times, the tendency to use natural materials such as microorganisms [4], marine organisms [5], proteins [6,7], and plant extracts (PE)…”