Plasmolysis and permeability of alpha-irradiated epidermal cells ofAllium cepa L.

Stadelmann, E.; Wattendorff, Joachim

doi:10.1007/bf01254635

Cited by 11 publications

(9 citation statements)

References 29 publications

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“…Changes in rates of plasmolysis and/or deplasmolysis of intact plant cells in hypertonic as compared to control solutions have been associated with changes in membrane permeability (STADELMAN & WATTENDORF 1966, FENG 1973, FENG & UNGER 1972. In the present investigation plasmolysis was effected on upper epidermal sections of rose (cv Baccara) petals whose red colour facilitated microscopic observation.…”

Section: Membrane Permeability Studiesmentioning

confidence: 99%

Possible Membrane-Associated Effects in Gibberellic Acid and Phenylalanine-Induced Rose Coloration Enhancement

Zieslin

Leshem

Spiegelstein

et al. 1977

Acta Botanica Neerlandica

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When combined with GA. phenylalanine produced marked enhancement of red colouration of rose (cv Baccara) petals, this effect significantly exceeding that ofGA applied alone, while phenylalanine when administered alone was virtually ineffective in this respect. Parallel assays of phenyl-ammonialyase (PAL) activity indicated essentially similar endogenous levels of enzyme irrespective of treatment. These results are interpreted as indicating that mode of GA action in this system is probably not by direct PAL activation but rather via GA-inducedenhancement of membrane permeability thus providing more phenylalanine precursor for anthocyanidin pigment synthesis. This hypothesis is substantiated by the results of plasmolysis studies executed on rose petal epidermal strips in hypertonic solutions where GA significantly cut down time required to achieve a given degree of plasymolysis.

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Section: Membrane Permeability Studiesmentioning

confidence: 99%

Possible Membrane-Associated Effects in Gibberellic Acid and Phenylalanine-Induced Rose Coloration Enhancement

Zieslin

Leshem

Spiegelstein

et al. 1977

Acta Botanica Neerlandica

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“…Figure 1 presents a flow diagram which outlines the deplasmolysis technique used in these studies. The adaxial epidermis from the third bulb scale of Allium and the lower epidermis from the leaf midrib of Rhoeo were collected as previously described (3,21 18). In this way the times for 50% and 90% deplasmolysis were determined exactly.…”

Section: Methodsmentioning

confidence: 99%

“…In a single study, six intact Rhoeo plants in a controlled environment chamber each growing in a 15-cm pot containing mixtures of 2 parts soil, 1 part sand, and 1 part peat moss (v/v) were treated daily with 1 mm DSA, pH 7, (200 ml of soil application per pot and foliar spray to run off) for 21 days. Six control plants randomly distributed in the same chamber were treated similarly with distilled water.…”

Section: Methodsmentioning

confidence: 99%

Influence of Decenylsuccinic Acid on Water Permeability of Plant Cells

1972

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“…Permeability determinations were made by the plasmometric method (12,21). After transferring the tissue to the perfusion chamber with buffer solution, the chamber was covered with a glass slip sealed with Vaseline and placed on the stage of a microscope.…”

Section: Methodsmentioning

confidence: 99%

“…The same buffer solution, less kinetin, was used for the pretreatment of the control tissue. All chemicals used were practical grade from Fisher Scientific Products, except kinetin (Calbiochem), and 1, 3-dimethylurea (Eastman Kodak).Permeability determinations were made by the plasmometric method (12,21). After transferring the tissue to the perfusion chamber with buffer solution, the chamber was covered with a glass slip sealed with Vaseline and placed on the stage of a microscope.…”

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confidence: 99%

Effects of Kinetin on the Permeability of Allium cepa Cells

Feng

1973

Plant Physiol.

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Permeability changes of Allium cepa cells were studied by a plasmometric method. Onion epidermis was floated in phosphate buffer solution with kinetin (2.5 milligrams/liter), or buffer without kinetin as a control, for 10 hours. The treated and control tissues were then transferred to one of the following four permeants: thiourea, urea, maloamide, dimethylurea.Kinetin treatment increased the permeability of onion epidermal cells to (14); further, the half-time for tritiated water efflux in both cylinders of carrot root tissue and of pelargonium stem pith is influenced by kinetin (6). This paper is a report of a study of kinetin effects on the permeability of onion epidermal cell membrane to four nonelectrolytes. MATERIALS AND METHODSThe inner epidermis of onion (Alliumn cepa) bulb scale was used. Epidermal tissue was cut into 12-x 15-mm strips. After floating the tissue on buffer solution for 10 hr in the dark at room temperature with or without kinetin, it was then transferred into a perfusion chamber. The flow of permeating solution through the chamber was kept constant by gravity.The phosphate buffer solution (2.5 mm, pH 6) for pretreatment contained 1 % sucrose with 2.5 mg/liter kinetin. The same buffer solution, less kinetin, was used for the pretreatment of the control tissue. All chemicals used were practical grade from Fisher Scientific Products, except kinetin (Calbiochem), and 1, 3-dimethylurea (Eastman Kodak).Permeability determinations were made by the plasmometric method (12,21). After transferring the tissue to the perfusion chamber with buffer solution, the chamber was covered with a glass slip sealed with Vaseline and placed on the stage of a microscope. The permeating solutions were 1 M thiourea, 1 M urea, 0.9 M malonamide, and 0.8 M 1, 3-dimethylurea. For the experiments with urea and malonamide, the hypertonic solutions were introduced directly into the chamber containing pretreated tissues. The solutions, at first. caused plasmolysis of the cells. As the permeants entered the cell, the protoplast expanded. The deplasmolysis is assumed to result from a permeation of the "plasmolyticum" into the treated cell. For experiments with the other two compounds (thiourea, dimethylurea) pretreated tissues were first plasmolyzed with 1 M mannitol and were then subjected to each of the permeants for observation of deplasmolysis. As the permeant was introduced into the perfusion chamber, a time lapse camera mounted on the microscope photographed the onion cells at given intervals.The camera was equipped with an auxiliary lens system to photograph a watch, automatically recording the time for each frame.Cells nearly cylindrical in shape were selected for study. The lengths of the protoplasts were measured on the photographic negative by means of a low magnification dissecting microscope and an eyepiece micrometer. This was done as soon as the protoplast was perfectly plasmolyzed, at which time both ends were clearly rounded off. Measurements were made for each frame, and the time was recorded.Usi...

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Plasmolysis and permeability of alpha-irradiated epidermal cells ofAllium cepa L.

Cited by 11 publications

References 29 publications

Possible Membrane-Associated Effects in Gibberellic Acid and Phenylalanine-Induced Rose Coloration Enhancement

Possible Membrane-Associated Effects in Gibberellic Acid and Phenylalanine-Induced Rose Coloration Enhancement

Influence of Decenylsuccinic Acid on Water Permeability of Plant Cells

Effects of Kinetin on the Permeability of Allium cepa Cells

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