Plasmodium vivax gametocytes in the bone marrow of an acute malaria patient and changes in the erythroid miRNA profile

Baró, Bàrbara; Deroost, Katrien; Raiol, Tainá; Brito, Marcelo Augusto Mota; Almeida, Anne Cristine Gomes de; Menezes-Neto, Armando de; Figueiredo, Erick Frota Gomes; Alencar, Aline; Leitão, Rodrigo; Val, Fernando; Monteiro, Wuelton Marcelo; Oliveira, Anna; Armengol, Maria del Pilar; Fernández-Becerra, Carmen; Lacerda, Marcus Vinícius Guimarães; Portillo, Hernando A. del

doi:10.1371/journal.pntd.0005365

Cited by 76 publications

(87 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reducing miR-451 relates to Ago2 in extracellular vesicles (EVs) to stimulate oxidative damage in infected-RBCs [ 65 ]. Interestingly, Baro et al [ 66 ] demonstrated that miR-221/222, -24 and -191 were decreased in bone marrow in P. vivax malaria patients. The numbers of P. falciparum patients need to more large scale study.…”

Section: Malaria and Mirnasmentioning

confidence: 99%

Circulating microRNAs in malaria infection: bench to bedside

Chamnanchanunt

Fucharoen

Umemura

2017

Malar J

View full text Add to dashboard Cite

Severe malaria has a poor prognosis with a morbidity rate of 80% in tropical areas. The early parasite detection is one of the effective means to prevent severe malaria of which specific treatment strategies are limited. Many clinical characteristics and laboratory testings have been used for the early diagnosis and prediction of severe disease. However, a few of these factors could be applied to clinical practice. MicroRNAs (miRNAs) were demonstrated as useful biomarkers in many diseases such as malignant diseases and cardiovascular diseases. Recently it was found that plasma miR-451 and miR-16 were downregulated in malaria infection at parasitic stages or with multi-organ failure involvement. MiR-125b, -27a, -23a, -150, 17–92 and -24 are deregulated in malaria patients with multiple organ failures. Here, the current findings of miRNAs were reviewed in relation to clinical severity of malaria infection and emphasized that miRNAs are potential biomarkers for severe malaria infection.

show abstract

Section: Malaria and Mirnasmentioning

confidence: 99%

Circulating microRNAs in malaria infection: bench to bedside

Chamnanchanunt

Fucharoen

Umemura

2017

Malar J

View full text Add to dashboard Cite

show abstract

“…were down-regulated while hsa-miR-144 was increased. However, hsa-miR-451a, the most abundant erythroid miRNA, remained stable [62]. In experimental cerebral malaria (ECM), an investigation in brain tissue found a higher expression of miR-150 while in the microvesicles from ECM mice showed non statistically different change of the miR-150 [42,50].…”

Section: Discussionmentioning

confidence: 99%

The characterization of extracellular vesicles-derived microRNAs in Thai malaria patients

Ketprasit

Cheng

Deutsch

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Extracellular vesicles (EVs) have been broadly studied in malaria for nearly a decade. These vesicles carry various functional biomolecules including RNA families such as microRNAs (miRNA). These EVs-derived microRNAs have numerous roles in host-parasite interactions and are considered as promising biomarkers for disease severity. However, this field lacks clinical studies of malaria-infected samples. In our study, we investigated EV specific miRNAs isolated from the plasma of patients from Thailand infected with Plasmodium falciparum and Plasmodium vivax . We postulated that these miRNAs were differentially expressed in these groups of patients and had a role in disease onset through the regulation of specific target genes. Methods EVs were purified from the plasma of Thai P.vivax -infected patients (PV, n=19), P.falciparum -infected patients (PF, n=18) and uninfected individuals (n=20). EV-derived miRNAs were then prepared and abundance of miR-15b-5p, hsa-miR-16-5p, hsa-let-7a-5p and hsa-miR-150-5p was assessed in these samples. Quantitative Polymerase Chain Reaction was performed, and relative expression of each miRNA was calculated using hsa-miR-451a as endogenous control. We then predicted the targets of up-regulated miRNAs and relevant pathways by bioinformatics. Receiver Operating Characteristic with Area Under the Curve (AUC) was then calculated to assess their diagnostic potential. Results The relative expression of hsa-miR-150-5p and hsa-miR-15b-5p was higher in PV patients compared to uninfected individuals, but hsa-let-7a-5p was up-regulated in both PV and PF. Bioinformatic analysis revealed that these miRNAs might regulate genes involved in the malaria pathway including the Adherens junction and the transforming growth factor-β pathways. All up-regulated miRNAs could potentially be used as disease biomarker as determined by AUC; however, the sensitivity and specificity require further investigation. Conclusion The up-regulated hsa-miR-150-5p and hsa-miR-15b-5p in PV, hsa-let-7a-5p in both PV and PF may play a role in host-parasite interactions. The findings in this study will require further validation in larger cohort groups of malaria patients to fully understand the contribution of these EV miRNAs to malaria detection and biology.

show abstract

“…Only in the last decade has Plasmodium transmission biology research re‐gained great momentum, leading to a large number of questions regarding Plasmodium interactions in the bone marrow, including mechanisms of extravasation, development and egress (Aguilar et al, ; Aingaran et al, ; Farfour et al, ; Joice et al, ; Lavazec, ; Lavazec et al, ; Lavazec & Alano, ; Messina et al, ; Naissant et al, ; Neveu et al, ; Ramdani et al, ; Rogers et al, ). Beyond P. falciparum , the bone marrow has been shown to be an important niche for P. vivax development, including gametocyte maturation and asexual development responsible for chronicity (Baro et al, ; Markus, ; Mayor & Alano, ; Obaldia et al, ).…”

Section: Primary Lymphoid Organs In Parasitic Infectionsmentioning

confidence: 99%

“…However, histology techniques combined with human and animal autopsies have shown that the bone marrow can influence pathology related to parasitic infections and in some cases, is even a key niche for parasite development. Direct and indirect bone marrow involvement in pathology has been suggested for parasites including Plasmodium spp., (Alano, 2017;Baro et al, 2017;De Niz et al, 2018;Duffier et al, 2016;Farfour et al 2012;Joice et al, 2014;Lee et al, 2017;Lee et al, 2018;Mayor & Alano, 2015;Messina et al, 2018;Neveu et al, 2018;Rogers et al, 2000;Smalley et al 1981;Waseem et al, 2016;Wickramasinghe et al, 1987), Toxoplasma gondii (Brouland et al, 1996), Leishmania spp., (Ali & Hussain, 2014;Hellal & Kundu, 2013;Kumar et al, 2007), Schistosoma spp., (Azevedo et al, 2015;Jones & Leday, 2014;Kamal et al 1989) and Trypanosoma spp. (Baena Terán et al, 2012;Bockstal, Guirnalda, et al, 2011;Bockstal, Geurts, & Magez, 2011;Carbajosa et al, 2017;De Diego et al 1998;Felizardo et al, 2018;Habila et al, 2014;Mabbott & Sternberg, 1995;Müller et al, 2018;Obishakin et al 2014;Souza et al, 2014;Stijlemans et al, 2016).…”

Section: Biological Relevance Of the Bone Marrowmentioning

confidence: 99%

See 1 more Smart Citation

Intravital microscopy: Imaging host–parasite interactions in lymphoid organs

Niz¹,

Meehan

Tavares

2019

Cellular Microbiology

View full text Add to dashboard Cite

Intravital microscopy allows imaging of biological phenomena within living animals, including host–parasite interactions. This has advanced our understanding of both, the function of lymphoid organs during parasitic infections, and the effect of parasites on such organs to allow their survival. In parasitic research, recent developments in this technique have been crucial for the direct study of host–parasite interactions within organs at depths, speeds and resolution previously difficult to achieve. Lymphoid organs have gained more attention as we start to understand their function during parasitic infections and the effect of parasites on them. In this review, we summarise technical and biological findings achieved by intravital microscopy with respect to the interaction of various parasites with host lymphoid organs, namely the bone marrow, thymus, lymph nodes, spleen and the mucosa‐associated lymphoid tissue, and present a view into possible future applications.

show abstract

Plasmodium vivax gametocytes in the bone marrow of an acute malaria patient and changes in the erythroid miRNA profile

Cited by 76 publications

References 16 publications

Circulating microRNAs in malaria infection: bench to bedside

Circulating microRNAs in malaria infection: bench to bedside

The characterization of extracellular vesicles-derived microRNAs in Thai malaria patients

Intravital microscopy: Imaging host–parasite interactions in lymphoid organs

Contact Info

Product

Resources

About