“…All the effective inhibitors bind strongly to DNA [39], and so they most likely interfere with PfRuvB3 unwinding function, whose domain ought to be distant from that of ATPase. PfRuvB3 is more sensitive to daunorubicin (IC 50 of 0.76 μM) than other helicases, such as P. falciparum DNA helicase A (PfDH A) [22], P. falciparum UvrD helicase (PfUDN) [40], P. falciparum 45 kDa helicase (PfH45) [41], P. falciparum 60 kDa DNA helicase (PfDH60) [42] and human DNA helicase (HDH II) [43] (IC 50s of 2.0, 4.4, 5.0 3.0, 6.23 μM, respectively). However, it is less sensitive to netropsin (IC 50 of 7.09 μM) than PfUDN, PfH45, PfDH60 and P. falciparum Dbp5/DDX19 homolog (PfD66) [44] (IC 50s of 3.3, 0.8, 0.5, 3.2 μM, respectively).…”