Plasmodium falciparum: - The Pf332 Antigen Is Secreted from the Parasite by a Brefeldin A-Dependent Pathway and Is Translocated to the Erythrocyte Membrane via the Maurer′s Clefts

Hinterberg, Katherine; Scherf, Artur; Gysin, Jürg; Toyoshima, Tetsuhiko; Aikawa, Masamichi; Mazié, J. C.; DaSilva, Lucie; Mattei, Denise

doi:10.1006/expr.1994.1091

Cited by 102 publications

(97 citation statements)

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“…The identification of such an activity supports the presence of a classical eukaryotic transport pathway involving coated vesicules in malarial parasite which has been suggested by BFA-inhibition experiences (Crary & Haldar 1992, Benting et al 1994, Das et al 1994, Hinterberg et al 1994, Ogun & Holder 1995, Howard & Schmidt 1995 and P. falciparum Arf or Arl (ADP-ribosylation factorlike) characterization (Stafford et al 1996, Lee et al 1997, Truong et al 1997.…”

Section: A B Csupporting

confidence: 54%

Plasmodium yoelii: identification of a gene encoding a putative ADP-ribosylation factor-1 GTPase-activating Protein, PyAG1

Hienne

Rico

Parzy

et al. 2000

Mem. Inst. Oswaldo Cruz

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Section: A B Csupporting

confidence: 54%

Plasmodium yoelii: identification of a gene encoding a putative ADP-ribosylation factor-1 GTPase-activating Protein, PyAG1

Hienne

Rico

Parzy

et al. 2000

Mem. Inst. Oswaldo Cruz

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“…The parasite contains itself within a self-constructed parasitophorous vacuole (PV), and forms a tubovesicular membrane (TVM) network that extends throughout the erythrocyte cytoplasm and associates with flattened vesicular structures beneath the red-cell membrane, called the Maurer's clefts (MCs), which are believed to translocate proteins secreted from the parasite to the erythrocyte surface (Barnwell 1990;Hinterberg et al 1994). A more recent model describes the MCs as part of a continuous membrane network extending from the PV (Wickert et al 2003b).…”

mentioning

confidence: 99%

A Plasmodium Gene Family Encoding Maurer's Cleft Membrane Proteins: Structural Properties and Expression Profiling

Sam‐Yellowe

Florens²,

Johnson³

et al. 2004

Genome Res.

140

136

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Upon invasion of the erythrocyte cell, the malaria parasite remodels its environment; in particular, it establishes a complex membrane network, which connects the parasitophorous vacuole to the host plasma membrane and is involved in protein transport and trafficking. We have identified a novel subtelomeric gene family in Plasmodium falciparum that encodes 11 transmembrane proteins localized to the Maurer's clefts. Using coimmunoprecipitation and shotgun proteomics, we were able to enrich specifically for these proteins and detect distinct peptides, allowing us to conclude that four to 10 products were present at a given time. Nearly all of the Pfmc-2tm genes are transcribed during the trophozoite stage; this narrow time frame of transcription overlaps with the specific stevor and rif genes that are differentially expressed during the erythrocyte cycle. The description of the structural properties of the proteins led us to manually reannotate published sequences, and to detect potentially homologous gene families in both P. falciparum and Plasmodium yoelii yoelii, where no orthologs were predicted uniquely based on sequence similarity. These basic proteins with two transmembrane domains belong to a larger superfamily, which includes STEVORs and RIFINs

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“…12 MAP1 antisera also stained the membrane of PRBC in late stages, which may reflect the translocation of Pf332 to the PRBC membrane during the course of parasite maturation. 13 The highest titre (2610 3 ) was recorded with MAP1 antisera from BALB/c, which also displayed the highest ELISA titre. Titres of all sera in IFA were consistently lower than those obtained by ELISA (Table 1).…”

Section: Reactivity Of Map Antisera With Parasite Antigen In Ifamentioning

confidence: 94%

“…13 The Pf332 molecule is only partially sequenced (approximately 1400 amino acids) and consists entirely of degenerated undecamer repeats. 12,14,15 Pf332-reactive antibodies have been reported to inhibit parasite growth in vitro.…”

Section: Introductionmentioning

confidence: 99%

Immune responses in congenic mice to multiple antigen peptides based on defined epitopes from the malaria antigen Pf332

et al. 1996

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SUMMARYRepeat sequences from the Plasmodium falciparum blood stage antigen Pf 332 frequently comprise the pentapeptide VTEEI, an epitope recognized by certain parasite neutralizing antibodies. This B-cell epitope was assembled in an octavalent multiple antigen peptide (MAP) system either as trimers (VTEEI) 3 (MAP1) or as an integral part of a naturally occurring Pf332 undecamer repeat sequence SVTEEIAEEDK (MAP2). Characteristics of the immunogenicity of these subunit constructs were evaluated in H-2 congenic mice. MAP1 generated antibody responses in mice of the H-2 d , H-2 k and H-2 q haplotypes, but not in H-2 b or H-2 s mice, whereas MAP2 only induced antibodies in mice of H-2 k haplotype. When analysing T-cell responses induced by the MAP, lymph node cells from responder strains primed in vivo with MAP1 proliferated in response to restimulation with both MAP1 and the peptide (VTEEI) 3 . MAP2, however, did not induce a detectable T-cell proliferation. Additionally, the lack of antibody response to MAP1 in H-2 b mice could be circumvented by combining the MAP1 peptide and a H-2 b -restricted T-cell epitope in a diepitope MAP construct. Despite the fact that the motif VTEEI has not been identified in Pf332 sequences in the form of a trimer, MAP1 did induce Pf332 proteinreactive antibodies. Assembly of multimers of short defined epitopes in MAP constitutes an interesting approach for the design of polyvalent subunit immunogens.

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Plasmodium falciparum: - The Pf332 Antigen Is Secreted from the Parasite by a Brefeldin A-Dependent Pathway and Is Translocated to the Erythrocyte Membrane via the Maurer′s Clefts

Cited by 102 publications

References 0 publications

Plasmodium yoelii: identification of a gene encoding a putative ADP-ribosylation factor-1 GTPase-activating Protein, PyAG1

Plasmodium yoelii: identification of a gene encoding a putative ADP-ribosylation factor-1 GTPase-activating Protein, PyAG1

A Plasmodium Gene Family Encoding Maurer's Cleft Membrane Proteins: Structural Properties and Expression Profiling

Immune responses in congenic mice to multiple antigen peptides based on defined epitopes from the malaria antigen Pf332

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