Plasmodium falciparum msp1 and msp2 genetic diversity and allele frequencies in parasites isolated from symptomatic malaria patients in Bobo-Dioulasso, Burkina Faso

Abstract

BackgroundIn Burkina Faso, malaria remains the overall leading cause of morbidity and mortality accounting for 35.12% of consultations, 40.83% of hospitalizations and 37.5% of deaths. Genotyping of malaria parasite populations remains an important tool to determine the types and number of parasite clones in an infection. The present study aimed to evaluate the merozoite surface protein 1 (msp1) and merozoite surface protein 2 (msp2) genetic diversity and allele frequencies in Bobo-Dioulasso, Burkina Faso.MethodDried blood spots (DBS) were collected at baseline from patients with uncomplicated malaria in urban health centers in Bobo-Dioulasso. Parasite DNA was extracted using chelex-100 and species were identified using nested PCR. Plamodium falciparum msp1 and msp2 genes were amplified by nested polymerase chain reaction (PCR) and PCR products were analyzed by electrophoresis on a 2.5% agarose gel. Alleles were categorized according to their molecular weight.ResultsA total of 228 blood samples were analyzed out of which 227 (99.9%) were confirmed as P. falciparum-positive and one sample classified as mixed infection for P. malaria and P. falciparum. In msp1, the K1 allelic family was predominant with 77.4% (162/209) followed respectively by the MAD20 allelic family with 41.3% and R033 allelic family with 36%. In msp2, the 3D7 allelic family was the most frequently detected with 93.1 % compared to FC27 with 41.3%. Twenty-one different alleles were observed in msp1 with 9 alleles for K1, 8 alleles for MAD20 and 4 alleles for R033. In msp2, 25 individual alleles were detected with 10 alleles for FC27 and 15 alleles for 3D7. The mean multiplicity of falciparum infection was 1.95 with respectively 1.8 (1.76–1.83) and 2.1 (2.03–2.16) for msp1 and msp2 (P = 0.01).ConclusionsOur study showed high genetic diversity and allelic frequencies of msp1 and msp2 in Plasmodium falciparum isolates from symptomatic malaria patients in Bobo-Dioulasso.

Genetic diversity and genotype multiplicity of Plasmodium falciparum infection in patients with uncomplicated malaria in Chewaka district, Ethiopia

“…In this study, 80% of the isolates harboured more than one parasite genotype identi ed by the presence of two or more alleles of one or both genes with the overall mean MOI being 3.2 (95% CI: 2.87-3.46). The overall MOI value reported in this study was higher than previously reported studies, including Ethiopia (MOI: 1.8 -2.6) between 2015-2018 [42,43,44], Brazzaville, Republic of Congo (MOI: 2.2) [16] in 2011 and Bobo-Dioulasso, Burkina Faso (MOI: 1.95) [62]. In contrast to study reported in Bioko Island, Equatorial Guinea (MOI: 5.51) [53] in 2018 and Gabon (MOI: 4.0) [63] in 2018.…”

Genetic Diversity and Complexity of Plasmodium Falciparum Infections in the Microenvironment Among Siblings of the Same Household in North-central Nigeria

“…On the whole, we found that about 65% of the study participants had polyclonal infections consisting of 2-6 clones with an overall MOI of 2.31 clones per infected child. This observation was not different from our previous report [31] and is also consistent with other data from Nigeria [40,41] and from other parts of Africa [14,24,[42][43][44][45][46][47][48][49][50]. The simultaneous infection of large number of individuals with multiple parasite genotypes in areas of high transmission intensity has been suggested to be attributable to either multiple inoculations of single clones, or by a single inoculation of multiple clones that may have undergone crossing and recombination in the female Anopheles mosquito vector [51,52].…”

Effect of irradiation on the survival and susceptibility of femaleAnopheles arabiensisto natural isolates ofPlasmodium falciparum

“…Because the characterization of vector competence for oocyst and sporozoite stages partly relied on different gametocyte carriers (Table 1), such isolate-dependent effect of irradiation could also explain why, on average, the sporozoite infection rate of irradiated individuals was not significantly lower than that of unirradiated-control individuals (Figure 1C). Here, we used wild parasite isolates from a geographic area characterized by an important genetic diversity (Somé et al ., 2018). Accordingly, some parasite clones might perform well in irradiated mosquitoes while others would be more infective to non-irradiated mosquitoes.…”